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Product Name
ANP32B antibody
- Documents
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Description
ANP32B Mouse Monoclonal antibody. Positive IF detected in HepG2 cells. Positive WB detected in HEK-293 cells, fetal human brain tissue, HeLa cells. Observed molecular weight by Western-blot: 29 kDa
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Tested applications
ELISA, WB, IF
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Species reactivity
Human; other species not tested.
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Alternative names
ANP32B antibody; APRIL antibody; PHAPI2 antibody; Silver stainable protein SSP29 antibody; SSP29 antibody
- Immunogen
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Isotype
Mouse IgG2b
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Preparation
This antibody was obtained by immunization of ANP32B recombinant protein (Accession Number: NM_006401). Purification method: Protein A purified.
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Clonality
Monoclonal
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Formulation
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
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Storage instructions
Store at -20℃. DO NOT ALIQUOT
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Applications
Recommended Dilution:
WB: 1:500-1:5000
IF: 1:50-1:500
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Validations
HEK-293 cells were subjected to SDS PAGE followed by western blot with Catalog No:107059(ANP32B Antibody) at dilution of 1:1000
HeLa cells were subjected to SDS PAGE followed by western blot with Catalog No:107059(ANP32B Antibody) at dilution of 1:5000
fetal human brain tissue were subjected to SDS PAGE followed by western blot with Catalog No:107059(ANP32B Antibody) at dilution of 1:1000
Immunofluorescent analysis of HepG2 cells using Catalog No:107059(ANP32B Antibody) at dilution of 1:50 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L)
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Background
ANP32B is a member of the highly conserved acidic leucine-rich nuclear phosphoprotein 32 (ANP32) family. The ANP32 proteins have been implicated in a broad array of physiological processes, including cell differentiation, apoptotic cell death, and cell proliferation. ANP32B was identified as a novel direct substrate for caspase-3 and acted as a negative regulator for leukemic cell apoptosis. ANP32B controls the expression of the dendritic cell maturation factor CD83 by regulating the transport of its mRNA to the cytoplasm. It also modulates the activity of the transcription factor Kruppel-like factor 5 (KLF5).
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