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BBa_J96012 vector

Basic Vector Information

Basic Vector Information
Vector Name BBa_J96012 Antibiotic Resistance Ampicillin
Length 3659 bp Type Biobrick cloning vector
Source Constante M, Grunberg R, Isalan M.

BBa_J96012 vector Vector Map

BBa_J96012 vector Sequence

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Download SnapGene Map(.dna)

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LOCUS       Exported                3659 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Biobrick cloning vector BBa_J96012, complete sequence.
ACCESSION   JN204881
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Constante M, Grunberg R, Isalan M.
  TITLE     A biobrick library for cloning custom eukaryotic plasmids
  JOURNAL   PLoS ONE 6 (8), E23685 (2011)
  PUBMED    21901127
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Constante M, Grunberg R, Isalan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2011) Systems Biology, CRG, C/ Dr. Aiguader, 88, 
            Barcelona, Barcelona 08003, Spain
REFERENCE   3  (bases 1 to 3659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Biobrick cloning vector BBa_J96012"
                     /mol_type="other DNA"
                     /label=pSB1AC3 backbone
                     /note="pSB1AC3 backbone"
                     /db_xref="taxon:1084337"
     misc_feature    1..22
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
     misc_feature    1..21
                     /label=Biobrick RFC23 format prefix
                     /note="Biobrick RFC23 format prefix"
     misc_feature    22..627
                     /label=FPuromycin Biobrick sequence
                     /note="FPuromycin Biobrick sequence"
     CDS             28..627
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     misc_feature    628..647
                     /label=Biobrick RFC23 format suffix
                     /note="Biobrick RFC23 format suffix"
     primer_bind     complement(783..802)
                     /note="VR reverse sequencing primer annealing site 
                     (BBa_G00102)"
     rep_origin      complement(889..1501)
                     /direction=LEFT
                     /label=pMB1
                     /note="pMB1"
     rep_origin      complement(902..1490)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     terminator      1673..1767
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(1788..2447)
                     /codon_start=1
                     /gene="chl"
                     /product="chloramphenicol resistance protein"
                     /label=chl
                     /note="confers resistance to chloramphenicol"
                     /protein_id="AEO27265.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     gene            complement(1788..2447)
                     /gene="chl"
                     /label=chl
     CDS             complement(2559..3419)
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /note="confers resistance to ampicillin"
                     /protein_id="AEO27266.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2559..3419)
                     /gene="amp"
                     /label=amp
     promoter        complement(3420..3524)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3542..3561
                     /note="VF2 forward sequencing primer annealing site 
                     (BBa_G00100)"
     terminator      3614..3657
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"

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