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BBa_J96001 vector

Basic Vector Information

Basic Vector Information
Vector Name BBa_J96001 Antibiotic Resistance Ampicillin
Length 3198 bp Type Biobrick cloning vector
Source Constante M, Grunberg R, Isalan M.

BBa_J96001 vector Vector Map

BBa_J96001 vector Sequence

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LOCUS       Exported                3198 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Biobrick cloning vector BBa_J96001, complete sequence.
ACCESSION   JN204870
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Constante M, Grunberg R, Isalan M.
  TITLE     A biobrick library for cloning custom eukaryotic plasmids
  JOURNAL   PLoS ONE 6 (8), E23685 (2011)
  PUBMED    21901127
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Constante M, Grunberg R, Isalan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2011) Systems Biology, CRG, C/ Dr. Aiguader, 88, 
            Barcelona, Barcelona 08003, Spain
REFERENCE   3  (bases 1 to 3198)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Biobrick cloning vector BBa_J96001"
                     /mol_type="other DNA"
                     /label=pSB1AK3 backbone
                     /note="pSB1AK3 backbone"
                     /db_xref="taxon:1084326"
     misc_feature    1..21
                     /label=Biobrick RFC23 format prefix
                     /note="Biobrick RFC23 format prefix"
     misc_feature    1..20
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     misc_feature    22..32
                     /label=Stop Biobrick sequence
                     /note="Stop Biobrick sequence"
     misc_feature    33..52
                     /label=Biobrick RFC23 format suffix
                     /note="Biobrick RFC23 format suffix"
     primer_bind     complement(188..207)
                     /note="VR reverse sequencing primer annealing site 
                     (BBa_G00102)"
     rep_origin      complement(294..906)
                     /direction=LEFT
                     /label=pMB1
                     /note="pMB1"
     rep_origin      complement(307..895)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(1128..1943)
                     /codon_start=1
                     /gene="kan"
                     /product="kanamycin resistance protein"
                     /label=kan
                     /note="confers resistance to kanamycin"
                     /protein_id="AEO27243.1"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(1128..1943)
                     /gene="kan"
                     /label=kan
     CDS             complement(2098..2958)
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /note="confers resistance to ampicillin"
                     /protein_id="AEO27244.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2098..2958)
                     /gene="amp"
                     /label=amp
     promoter        complement(2959..3063)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3081..3100
                     /note="VF2 forward sequencing primer annealing site 
                     (BBa_G00100)"
     terminator      3153..3196
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"

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