NovoPro
Cart 2

BBa_96046 vector

Basic Vector Information

Basic Vector Information
Vector Name BBa_96046 Antibiotic Resistance Ampicillin
Length 3573 bp Type Biobrick cloning vector
Source Constante M, Grunberg R, Isalan M.

BBa_96046 vector Vector Map

BBa_96046 vector Sequence

Copy Sequence

Download SnapGene Map(.dna)

Download GeneBank File(.gb)

LOCUS       Exported                3573 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Biobrick cloning vector BBa_96046, complete sequence.
ACCESSION   JN204910
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Constante M, Grunberg R, Isalan M.
  TITLE     A biobrick library for cloning custom eukaryotic plasmids
  JOURNAL   PLoS ONE 6 (8), E23685 (2011)
  PUBMED    21901127
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Constante M, Grunberg R, Isalan M.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-JUL-2011) Systems Biology, CRG, C/ Dr. Aiguader, 88, 
            Barcelona, Barcelona 08003, Spain
REFERENCE   3  (bases 1 to 3573)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 18, 2018 from SnapGene 4.1.8
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Biobrick cloning vector BBa_96046"
                     /mol_type="other DNA"
                     /label=pSB1AT3 backbone
                     /note="pSB1AT3 backbone"
                     /db_xref="taxon:1084323"
     misc_feature    1..21
                     /label=Biobrick RFC23 format prefix
                     /note="Biobrick RFC23 format prefix"
     misc_feature    1..20
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     misc_feature    22..150
                     /label=d1PEST Biobrick sequence
                     /note="d1PEST Biobrick sequence"
     misc_feature    151..170
                     /label=Biobrick RFC23 format suffix
                     /note="Biobrick RFC23 format suffix"
     primer_bind     complement(306..325)
                     /note="VR reverse sequencing primer annealing site 
                     (BBa_G00102)"
     rep_origin      complement(412..1024)
                     /direction=LEFT
                     /label=pMB1
                     /note="pMB1"
     rep_origin      complement(425..1013)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        1200..1228
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             1276..2466
                     /codon_start=1
                     /gene="tet"
                     /product="tetracyclin resistance protein"
                     /label=tet
                     /note="confers resistance to tetracyclin"
                     /protein_id="AEO27323.1"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     gene            1276..2466
                     /gene="tet"
                     /label=tet
     CDS             complement(2473..3333)
                     /codon_start=1
                     /gene="amp"
                     /product="ampicillin resistance protein"
                     /label=amp
                     /note="confers resistance to ampicillin"
                     /protein_id="AEO27324.1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     gene            complement(2473..3333)
                     /gene="amp"
                     /label=amp
     promoter        complement(3334..3438)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     3456..3475
                     /note="VF2 forward sequencing primer annealing site 
                     (BBa_G00100)"
     terminator      3528..3571
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"

This page is informational only.