AAV-hSyn-Jaws-mRuby2-8X2C vector (V010130)

Basic Vector Information

      • Vector Name:
      • AAV-hSyn-Jaws-mRuby2-8X2C
      • Length:
      • 3798 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Tseng H-a., Ta T, Gritton HJ, Man H-Y.

AAV-hSyn-Jaws-mRuby2-8X2C vector Vector Map

AAV-hSyn-Jaws-mRuby2-8X2C3798 bp600120018002400300036005'ITRhSynJaws-mRUby2-ER2miRNAWPREKS primerSV40 poly(A) signal3'ITR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AAV-hSyn-Jaws-mRuby2-8X2C vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3798 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector AAV-hSyn-Jaws-mRuby2-8X2C, complete sequence.
ACCESSION   MH458081
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Tseng H-a., Ta T, Gritton HJ, Man H-Y.
  TITLE     A microRNA-based gene targeting tool for virally labeling 
            interneurons in the rodent cortex
  JOURNAL   Cell Rep (2018) In press
REFERENCE   2  (bases 1 to 3798)
  AUTHORS   Keaveney MK, Han X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2018) Biomedical Engineering, Boston University, 
            610 Commonwealth Ave, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 3798)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: APE Plasmid Editor v. v2.0.53.c
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Cloning vector AAV-hSyn-Jaws-mRuby2-8X2C"
                     /mol_type="other DNA"
                     /db_xref="taxon:2250249"
     repeat_region   1..101
                     /label=5'ITR
                     /note="5'ITR"
     regulatory      197..677
                     /regulatory_class="promoter"
                     /note="hSyn"
     promoter        224..671
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      684..692
                     /regulatory_class="ribosome_binding_site"
     CDS             696..2270
                     /codon_start=1
                     /product="Jaws-mRUby2-ER2"
                     /label=Jaws-mRUby2-ER2
                     /protein_id="AWX65709.1"
                     /translation="TAVSTTATTVLQATQSDVLQEIQSNFLLNSSIWVNIALAGVVILL
                     FVAMGRDLESPRAKLIWVATMLVPLVSISSYAGLASGLTVGFLQMPPGHALAGQEVLSP
                     WGRYLTWTFSTPMILLALGLLADTDIASLFTAITMDIGMCVTGLAAALITSSHLLRWVF
                     YGISCAFFVAVLYVLLVQWPADAEAAGTSEIFGTLRILTVVLWLGYPILFALGSEGVAL
                     LSVGVTSWGYSGLDILAKYVFAFLLLRWVAANEGTVSGSGMGIGSGGAAPADDRPVVSK
                     GEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMRIKVIEGGPLPFAFDILA
                     TSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGVVTVMQDTSLEDGCLVYH
                     VQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHMALKVDGGGHLSCSFVTT
                     YRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVAKFAGLGGGMDELYKLYK
                     FCYENEV"
     CDS             1530..2237
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="VSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     ncRNA           complement(2270..2285)
                     /label=miRNA
                     /note="microRNA"
                     /ncRNA_class="miRNA"
     misc_feature    2748..3336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    2750..3337
                     /label=WPRE
                     /note="WPRE"
     CDS             complement(3219..3230)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3339..3355)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    3385..3506
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3609..3798
                     /label=3'ITR
                     /note="3'ITR"

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