AAV-CamKIIalpha-mRuby2 vector (V010131)

Basic Vector Information

      • Vector Name:
      • AAV-CamKIIalpha-mRuby2
      • Length:
      • 3300 bp
      • Type:
      • Cloning vector
      • Source/Author:
      • Tseng H-a., Ta T, Gritton HJ, Man H-Y.

AAV-CamKIIalpha-mRuby2 vector Vector Map

AAV-CamKIIalpha-mRuby23300 bp60012001800240030005'ITRCamKIIalphamRuby2WPREKS primerSV40 poly(A) signal3'ITR

Plasmid Resuspension Protocol:

1. Centrifuge at 5,000×g for 5 min.

2. Carefully open the tube and add 20 μl of sterile water to dissolve the DNA.

3. Close the tube and incubate for 10 minutes at room temperature.

4. Briefly vortex the tube and then do a quick spin to concentrate the liquid at the bottom. Speed is less than 5000×g.

5.Store the plasmid at -20 ℃.

AAV-CamKIIalpha-mRuby2 vector Sequence

Copy Sequence

Download GeneBank File(.gb)

LOCUS       Exported                3300 bp ds-DNA     circular SYN 17-DEC-2018
DEFINITION  Cloning vector AAV-CamKIIalpha-mRuby2, complete sequence.
ACCESSION   MH458080
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Tseng H-a., Ta T, Gritton HJ, Man H-Y.
  TITLE     A microRNA-based gene targeting tool for virally labeling 
            interneurons in the rodent cortex
  JOURNAL   Cell Rep (2018) In press
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Keaveney MK, Han X.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-JUN-2018) Biomedical Engineering, Boston University, 
            610 Commonwealth Ave, Boston, MA 02215, USA
REFERENCE   3  (bases 1 to 3300)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Assembly Method       :: APE Plasmid Editor v. v2.0.53.c
            Sequencing Technology :: Illumina
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Cloning vector AAV-CamKIIalpha-mRuby2"
                     /mol_type="other DNA"
                     /db_xref="taxon:2250246"
     repeat_region   1..101
                     /label=5'ITR
                     /note="5'ITR"
     regulatory      197..1487
                     /regulatory_class="promoter"
                     /note="CamKIIalpha"
     regulatory      1493..1504
                     /regulatory_class="ribosome_binding_site"
     regulatory      1499..1508
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             1505..2215
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby, with improved photophysical properties (Lam et al., 
                     2012)"
                     /label=mRuby2
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTH
                     MALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAV
                     AKFAGLGGGMDELYK"
     CDS             1508..2218
                     /codon_start=1
                     /product="mRuby2"
                     /label=mRuby2
                     /protein_id="AWX65708.1"
                     /translation="MSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGNPYMGTQTMR
                     IKVIEGGPLPFAFDILATSFMYGSRTFIKYPKGIPDFFKQSFPEGFTWERVTRYEDGGV
                     VTVMQDTSLEDGCLVYHVQVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTHM
                     ALKVDGGGHLSCSFVTTYRSKKTVGNIKMPGIHAVDHRLERLEESDNEMFVVQREHAVA
                     KFAGLGGGMDELYK"
     misc_feature    2250..2838
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     misc_feature    2252..2839
                     /label=WPRE
                     /note="WPRE"
     CDS             complement(2721..2732)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2841..2857)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2887..3008
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     repeat_region   3111..3300
                     /label=3'ITR
                     /note="3'ITR"

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