LOCUS       40924_49787        6299 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Expression vector yrbE1AB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6299)
  AUTHORS   Joshi SM, Pandey AK, Capite N, Fortune SM, Rubin EJ, Sassetti CM.
  TITLE     Characterization of mycobacterial virulence genes through genetic 
            interaction mapping
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 103 (31), 11760-11765 (2006)
  PUBMED    16868085
REFERENCE   2  (bases 1 to 6299)
  AUTHORS   Joshi SM, Pandey AK, Capite NM, Fortune SM, Rubin EJ, Sassetti CM.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-JUN-2006) Molecular Genetics and Microbiology, 
            University of Massachusetts Medical School, 55 Lake Ave. North, 
            Worcester, MA 01655, USA
REFERENCE   3  (bases 1 to 6299)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6299)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "2006"; volume: "103"; issue: "31"; pages:
            "11760-11765"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-JUN-2006) Molecular Genetics and Microbiology, University of 
            Massachusetts Medical School, 55 Lake Ave. North, Worcester, MA 
            01655, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      7..394
                     /note="promoter from Mycobacterium tuberculosis groEL2
                     gene"
                     /regulatory_class="promoter"
     CDS             446..1243
                     /codon_start=1
                     /gene="yrbE1A"
                     /product="YrbE1A"
                     /label=yrbE1A
                     /protein_id="ABG72765.1"
                     /translation="MTTSTTLGGYVRDQLQTPLTLVGGFFRMCVLTGKALFRWPFQWRE
                     FILQCWFIMRVGFLPTIMVSIPLTVLLIFTLNILLAQFGAADISGSGAAIGAVTQLGPL
                     TTVLVVAGAGSTAICADLGARTIREEIDAMEVLGIDPIHRLVVPRVLASMLVATLLNGL
                     VITVGLVGGFLFGVYLQNVSGGAYLATLTLITGLPEVVIATIKAATFGLIAGLVGCYRG
                     LTVRGGSKGLGTAVNETVVLCVIALFAVNVILTTIGVRFGTGR"
     gene            446..1243
                     /gene="yrbE1A"
                     /label=yrbE1A
     CDS             2118..2144
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     terminator      2200..2246
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             complement(2298..3293)
                     /gene="hyg"
                     /label=hyg
                     /note="Hygromycin-B 7''-O-kinase from Streptomyces 
                     hygroscopicus. Accession#: P09979"
     rep_origin      3632..4220
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     rep_origin      4379..6273
                     /label=mycobacterial origin of replication from pAL5000
                     /note="mycobacterial origin of replication from pAL5000"
ORIGIN
        1 tctagaggtg accacaacga cgcgcccgct ttgatcgggg acgtctgcgg ccgaccattt
       61 acgggtcttg ttgtcgttgg cggtcatggg ccgaacatac tcacccggat cggagggccg
      121 aggacaaggt cgaacgaggg gcatgacccg gtgcggggct tcttgcactc ggcataggcg
      181 agtgctaaga ataacgttgg cactcgcgac cggtgagtcg taggtcggga cggtgaggcc
      241 aggcccgtcg tcgcagcgag tggcagcgag gacaacttga gccgtccgtc gcgggcactg
      301 cgcccggcca gcgtaagtag cggggttgcc gtcacccggt gacccccggt ttcatccccg
      361 atccggagga atcacttcgc aatggccatg gccaagacaa ttgcggatct ataaataccc
      421 aggtcggcgg cttgagggag ccgcggtgac gacgtcgaca acgcttggcg gttacgtccg
      481 cgaccaactg caaaccccgc tgaccctcgt cggtggattc tttcgcatgt gtgtgctgac
      541 tggaaaggcg ctgtttcgct ggccgttcca gtggcgcgag ttcattctgc agtgctggtt
      601 catcatgcgg gtcggatttt taccgacgat catggtctcg ataccgctga cggtgctgtt
      661 gatcttcacg ctcaatattc tgctggccca gttcggcgcg gcagacatct ccggttccgg
      721 cgcggcgatc ggcgcggtca cccagcttgg cccgctgaca acggtgctgg tggtcgccgg
      781 cgccggatcc acggccatct gcgccgacct gggtgcccgc accatccgcg aggaaatcga
      841 cgcgatggag gtgctgggca tcgatcccat ccaccgtctg gtggtgccgc gggtgctcgc
      901 ctcgatgctg gtcgccacgc tgctcaacgg cttggtgatc accgtcggcc tggtcggtgg
      961 ctttctcttc ggtgtctatc tgcagaacgt ttcgggcggc gcctaccttg ccacgctgac
     1021 cttgatcacc ggcctgcccg aggtggtcat cgcaaccatc aaagccgcaa cgttcggcct
     1081 gatcgcgggc cttgtcggct gctatcgggg gctgaccgtc cgtggcggtt ccaagggtct
     1141 tggcaccgcc gtcaacgaga ccgtggtgct gtgtgtgatt gccctgttcg ccgtcaacgt
     1201 gatcttgacg accatcggtg tgcgattcgg gacggggcgc tgacatgtcg accgctgctg
     1261 tgctgcgcgc ccgcttcccg cgggcggtcg ccaaccttcg tcaatatgga ggtgcggcgg
     1321 cccgtggatt ggacgaggcc ggccagctca cctggttcgc tttgaccagc atcgggcaga
     1381 tcgcgcacgc gctgcgctac taccgcaagg agacgctgcg gctgatcgcc cagatcggca
     1441 tgggtaccgg cgcgatggcc gtcgtcggcg gcacggtcgc catcgttggc tttgtcacgc
     1501 tgtccggcag ctcgctggtc gcaatccagg gcttcgcgtc gctgggcaac atcggtgtcg
     1561 aggcgttcac cgggttcttc gccgcactga tcaacgtgcg catcgccggc ccagttgtca
     1621 cgggtgtcgc cctggcggcc acggtcggtg cgggtgctac ggccgagctg ggcgcgatgc
     1681 ggatcagcga ggagatcgat gccctggaag tgatgggcat caagtcgatc tcgtttctgg
     1741 cctccacccg gatcatggcc gggctggtgg tgatcatccc gctgtacgcg ttggcgatga
     1801 ttatgtcgtt cctgtccccg cagatcacca ccacggtgct ctacgggcag tcgaacggca
     1861 cctacgagca ttactttcaa acgttcctgc gtcccgacga tgtcttttgg tccttcttgg
     1921 aggccctcat catcactgcg atcgtcatgg tcagccactg ctactacggg tacgccgccg
     1981 gtggaggccc cgtcggtgtc ggcgaggccg tcggccgatc gatgcgtttc tcgttggtct
     2041 cggtgcaggt cgttgtcctg tttgcagcgt tggcgctcta cggtgtcgac ccgaacttca
     2101 atctcacggt ggccgcctac ccgtacgacg tgccggacta cgcgtaaaag cttatcgatg
     2161 tcgacgtagt taactagcgt acgatcgact gccaggcatc aaataaaacg aaaggctcag
     2221 tcgaaagact gggcctttcg ttttatctgt tgtttgtccg gccatcatgg ccgcggtgat
     2281 cagctagagg ggcgtcaggc gccgggggcg gtgtccggcg gcccccagag gaactgcgcc
     2341 agttcctccg gatcggtgaa gccggagaga tccagcgggg tctcctcgaa cacctcgaag
     2401 tcgtgcagga aggtgaaggc gagcagttcg cgggcgaagt cctcggtccg cttccactgc
     2461 gccccgtcga gcagcgcggc caggatctcg cggtcgcccc ggaaggcgtt gagatgcagt
     2521 tgcaccaggc tgtagcggga gtctcccgca tagacgtcgg tgaagtcgac gatcccggtg
     2581 acctcggtcg cggccaggtc cacgaagatg ttggtcccgt gcaggtcgcc gtggacgaac
     2641 cggggttcgc ggccggccag cagcgtgtcc acgtccggca gccagtcctc caggcggtcc
     2701 agcagccggg gcgagaggta gccccacccg cggtggtcct cgacggtcgc cgcgcggcgt
     2761 tcccgcagca gttccgggaa gacctcggaa tggggggtga gcacggtgtt cccggtcagc
     2821 ggcaccctgt gcagccggcc gagcacccgg ccgagttcgc gggccagggc gagcagcgcg
     2881 ttccggtcgg tcgtgccgtc catcgcggac cgccaggtgg tgccggtcat ccggctcatc
     2941 accaggtagg gccacggcca ggctccggtg ccgggccgca gctcgccgcg gccgaggagg
     3001 cggggcaccg gcaccggggc gtccgccagg accgcgtacg cctccgactc cgacgcgagg
     3061 ctctccggac cgcaccagtg ctcgccgaac agcttgatca ccgggtcggg ctcgccgacc
     3121 agtacggggt tggtgctctc gccgggcacc cgcagcaccg gcggcaccgg cagcccgagc
     3181 tcctccaggg ctcggcgggc cagcggctcc cagaattcct ggtcgttccg caggctcgcg
     3241 taggaatcat ccgaatcaat acggtcgaga agtaacaggg attcttgtgt cacagcggac
     3301 ctctattcac agggtacggg ccggcttaat tccgcacggc cggtcgcgac acggcctgtc
     3361 cgcaccgcgg tcaggcgttg acgatgacgg gctggtcggc cacgtcgggg agtcaggcaa
     3421 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     3481 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     3541 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gctctagttc cactgagcgt
     3601 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     3661 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     3721 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     3781 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     3841 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     3901 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     3961 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     4021 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     4081 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     4141 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     4201 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     4261 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     4321 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcaacgcgt
     4381 gagcccacca gctccgtaag ttcgggtgct gtgtggctcg tacccgcgca ttcaggcggc
     4441 agggggtcta acgggtctaa ggcggcgtgt acggccgcca cagcggctct tagcggcccg
     4501 gaaacgtcct cgaaacgacg catgtgttcc tcctggttgg tacaggtggt tgggggtgct
     4561 cggctgtcgc tggtgtttca tcatcagggc tcgacgggag agcgggggag tgtgcagttg
     4621 tggggtggcc cctcagcgaa atatctgact tggagctcgt gtcggaccat acaccggtga
     4681 ttaatcgtgg tttattatca agcgtgagcc acgtcgccga cgaatttgag cagctctggc
     4741 tgccgtactg gtccctggca agcgacgatc tgctcgaggg gatctaccgc caaagccgcg
     4801 cgtcggccct aggccgccgg tacatcgagg cgaacccaac agcgctggca aacctgctgg
     4861 tcgtggacgt agaccatcca gacgcagcgc tccgagcgct cagcgcccgg gggtcccatc
     4921 cgctgcccaa cgcgatcgtg ggcaatcgcg ccaacggcca cgcacacgca gtgtgggcac
     4981 tcaacgcccc tgttccacgc accgaatacg cgcggcgtaa gccgctcgca tacatggcgg
     5041 cgtgcgccga aggccttcgg cgcgccgtcg atggcgaccg cagttactca ggcctcatga
     5101 ccaaaaaccc cggccacatc gcctgggaaa cggaatggct ccactcagat ctctacacac
     5161 tcagccacat cgaggccgag ctcggcgcga acatgccacc gccgcgctgg cgtcagcaga
     5221 ccacgtacaa agcggctccg acgccgctag ggcggaattg cgcactgttc gattccgtca
     5281 ggttgtgggc ctatcttccc gccctcatgc ggatctacct gccgacccgg aacgtggacg
     5341 gactcggccg cgcgatctat gccgagtgcc acgcgcgaaa cgccgaattt ccgtgcaacg
     5401 acgtgtgtcc cggaccgcta ccggacagcg aggtccgcgc catcgccaac agcatttggc
     5461 gttggatcac aaccaagtcg cgcatttggg cggacgggat cgtggtctac gaggccacac
     5521 tcagtgcgcg ccatgcggcc atctcgcgga agggcgcagc agcgcgcacg gcggcgagca
     5581 cagttgcgcg gcgcgcaaag tccgcgtcag ccatggaggc attgctatga gcgacggcta
     5641 cagcgacggc tacagcgacg gctacaactg gcagccgact gtccgcaaaa agcggcgcgt
     5701 gaccgccgcc gaaggcgctc gaatcaccgg actatccgaa cgccacgtcg tccggctcgt
     5761 ggcgcaggaa cgcagcgagt ggttcgccga gcaggctgca cgccgcgaac gcatccgcgc
     5821 ctatcacgac gacgagggcc actcttggcc gcaaacggcc aaacatttcg ggctgcatct
     5881 ggacaccgtt aagcgactcg gctatcgggc gaggaaagag cgtgcggcag aacaggaagc
     5941 ggctcaaaag gcccacaacg aagccgacaa tccaccgctg ttctaacgca attggggagc
     6001 gggtgtcgcg ggggttccgt ggggggttcc gttgcaacgg gtcggacagg taaaagtcct
     6061 ggtagacgct agttttctgg tttgggccat gcctgtctcg ttgcgtgttt cgttgcgtcc
     6121 gttttgaata ccagccagac gagacggggt tctacgaatc ttggtcgata ccaagccatt
     6181 tccgctgaat atcgtggagc tcaccgccag aatcggtggt tgtggtgatg tacgtggcga
     6241 actccgttgt agtgcttgtg gtggcatccg tggcgcggcc gcggtaccag atctttaaa
//
