LOCUS       40924_49727        5586 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector yGALset986, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Enomoto S, Chen G, Berman J.
  TITLE     Vectors for expressing T7 epitope- and His6 affinity-tagged fusion 
            proteins in S. cerevisiae
  JOURNAL   BioTechniques 24 (5), 782-786 (1998)
  PUBMED    9591127
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Enomoto S, Berman J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-1998) Plant Biology, University of Minnesota, 220 
            Biological Sciences Center, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 5586)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5586)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "24"; issue: "5"; pages: 
            "782-786"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-1998) Plant Biology, University of Minnesota, 220 Biological
            Sciences Center, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..88
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(121..785)
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     RBS             804..826
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             846..863
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             867..899
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             903..926
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     misc_feature    936..982
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     terminator      1046..1093
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      1193..1648
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     CDS             complement(1912..2568)
                     /codon_start=1
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
                     /translation="MTEQKALVKRITNETKIQIAISLKGGPLAIEHSIFPEKEAEAVAE
                     QATQSQVINVHTGIGFLDHMIHALAKHSGWSLIVECIGDLHIDDHHTTEDCGIALGQAF
                     KEALLARGVKRFGSGFAPLDEALSRAVVDLSNRPYAVVELGLQREKVGDLSCEMIPHFL
                     ESFAEASRITLHVDCLRGKNDHHRSESAFKALAVAIREATSPNGTNDVPSTKGVLM"
     promoter        complement(2569..2756)
                     /label=HIS3 promoter
     misc_feature    complement(3140..3643)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        3680..3784
                     /label=AmpR promoter
     CDS             3785..4642
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4816..5404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gatctcgatc
       61 ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct aggatccggg
      121 gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt
      181 attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt
      241 tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta
      301 attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg
      361 attcgttcat ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca
      421 taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc
      481 gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg
      541 ctgtcacccg ctcggcggct tctaatccgt acttcaatat agcaatgagc agttaagcgt
      601 attactgaaa gttccaaaga gaaggttttt ttaggctaag ataatggggc tctttacatt
      661 tccacaacat ataagtaaga ttagatatgg atatgtatat ggatatgtat atggtggtaa
      721 tgccatgtaa tatgattatt aaacttcttt gcgtccatcc aaaaaaaaag taagaatttt
      781 tgaaaattcg aattctagat aattttgttt aactttaaga aggagatata catatgcggg
      841 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc
      901 gggatctgta cgacgatgac gataaggatc gatggggatc cgagctcgag atctgcagct
      961 ggtaccatgg aattcgaagc ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt
     1021 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     1081 gaggggtttt ttgctgaaag gaggaactat atccggatct ggcgtaatag cgaagaggcc
     1141 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cgacgcgccc
     1201 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     1261 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     1321 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     1381 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     1441 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     1501 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     1561 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     1621 tttaacaaaa tattaacgtt tacaatttcc tgatgcggta ttttctcctt acgcatctgt
     1681 gcggtatttc acaccgcata tgatccgtcg agttcaagag aaaaaaaaag aaaaagcaaa
     1741 aagaaaaaag gaaagcgcgc ctcgttcaga atgacacgta tagaatgatg cattaccttg
     1801 tcatcttcag tatcatactg ttcgtataca tacttactga cattcatagg tatacatata
     1861 tacacatgta tatatatcgt atgctgcagc tttaaataat cggtgtcact acataagaac
     1921 acctttggtg gagggaacat cgttggtacc attgggcgag gtggcttctc ttatggcaac
     1981 cgcaagagcc ttgaacgcac tctcactacg gtgatgatca ttcttgcctc gcagacaatc
     2041 aacgtggagg gtaattctgc tagcctctgc aaagctttca agaaaatgcg ggatcatctc
     2101 gcaagagaga tctcctactt tctccctttg caaaccaagt tcgacaactg cgtacggcct
     2161 gttcgaaaga tctaccaccg ctctggaaag tgcctcatcc aaaggcgcaa atcctgatcc
     2221 aaaccttttt actccacgcg ccagtagggc ctctttaaaa gcttgaccga gagcaatccc
     2281 gcagtcttca gtggtgtgat ggtcgtctat gtgtaagtca ccaatgcact caacgattag
     2341 cgaccagccg gaatgcttgg ccagagcatg tatcatatgg tccagaaacc ctatacctgt
     2401 gtggacgtta atcacttgcg attgtgtggc ctgttctgct actgcttctg cctctttttc
     2461 tgggaagatc gagtgctcta tcgctagggg accacccttt aaagagatcg caatctgaat
     2521 cttggtttca tttgtaatac gctttactag ggctttctgc tctgtcatct ttgccttcgt
     2581 ttatcttgcc tgctcatttt ttagtatatt cttcgaagaa atcacattac tttatataat
     2641 gtataattca ttatgtgata atgccaatcg ctaagaaaaa aaaagagtca tccgctaggt
     2701 ggaaaaaaaa aaatgaaaat cattaccgag gcataaaaaa atatagagtg tactagagga
     2761 ggccaagagt aatagaaaaa gaaaattgcg ggaaaggact gtgttatgac ttccctgact
     2821 aatgccgtgt tcaaacgata cctggcagtg actcctagcg ctcaccaagc tcttaaaacg
     2881 gaattatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga
     2941 cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac
     3001 agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg
     3061 aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata
     3121 ataatggttt cttaggacgg atcgcttgcc tgtaacttac acgcgcctcg tatcttttaa
     3181 tgatggaata atttgggaat ttactctgtg tttatttatt tttatgtttt gtatttggat
     3241 tttagaaagt aaataaagaa ggtagaagag ttacggaatg aagaaaaaaa aataaacaaa
     3301 ggtttaaaaa atttcaacaa aaagcgtact ttacatatat atttattaga caagaaaagc
     3361 agattaaata gatatacatt cgattaacga taagtaaaat gtaaaatcac aggattttcg
     3421 tgtgtggtct tctacacaga caagatgaaa caattcggca ttaatacctg agagcaggaa
     3481 gagcaagata aaaggtagta tttgttggcg atccccctag agtcttttac atcttcggaa
     3541 aacaaaaact attttttctt taatttcttt ttttactttc tatttttaat ttatatattt
     3601 atattaaaaa atttaaatta taattatttt tatagcacgt gatgaaaagg acccaggtgg
     3661 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3721 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3781 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3841 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3901 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3961 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4021 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4081 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4141 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4201 gatcggagga ccgaaggagc taaccgcttt ttttcacaac atgggggatc atgtaactcg
     4261 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4321 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4381 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4441 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4501 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4561 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4621 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4681 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4921 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5461 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5521 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5581 ggaaga
//
