LOCUS       40924_49717        5731 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector yGALset984, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Enomoto S, Chen G, Berman J.
  TITLE     Vectors for expressing T7 epitope- and His6 affinity-tagged fusion 
            proteins in S. cerevisiae
  JOURNAL   BioTechniques 24 (5), 782-786 (1998)
  PUBMED    9591127
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Enomoto S, Berman J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-1998) Plant Biology, University of Minnesota, 220 
            Biological Sciences Center, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 5731)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5731)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "24"; issue: "5"; pages: 
            "782-786"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-1998) Plant Biology, University of Minnesota, 220 Biological
            Sciences Center, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..88
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(121..785)
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     RBS             804..826
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             846..863
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             867..899
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             903..926
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     misc_feature    936..982
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     terminator      1046..1093
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      complement(1485..2831)
                     /direction=LEFT
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(2946..3617)
                     /codon_start=1
                     /label=TRP1
                     /note="phosphoribosylanthranilate isomerase, required for 
                     tryptophan biosynthesis"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     promoter        complement(3618..3719)
                     /label=TRP1 promoter
     promoter        3825..3929
                     /label=AmpR promoter
     CDS             3930..4787
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4961..5549
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gatctcgatc
       61 ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct aggatccggg
      121 gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt
      181 attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt
      241 tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta
      301 attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg
      361 attcgttcat ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca
      421 taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc
      481 gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg
      541 ctgtcacccg ctcggcggct tctaatccgt acttcaatat agcaatgagc agttaagcgt
      601 attactgaaa gttccaaaga gaaggttttt ttaggctaag ataatggggc tctttacatt
      661 tccacaacat ataagtaaga ttagatatgg atatgtatat ggatatgtat atggtggtaa
      721 tgccatgtaa tatgattatt aaacttcttt gcgtccatcc aaaaaaaaag taagaatttt
      781 tgaaaattcg aattctagat aattttgttt aactttaaga aggagatata catatgcggg
      841 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc
      901 gggatctgta cgacgatgac gataaggatc gatggggatc cgagctcgag atctgcagct
      961 ggtaccatgg aattcgaagc ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt
     1021 ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt
     1081 gaggggtttt ttgctgaaag gaggaactat atccggatct ggcgtaatag cgaagaggcc
     1141 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg
     1201 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatcggat cgtacttgtt
     1261 acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca gatagtatat
     1321 ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta tgtatttcgg
     1381 ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca tccccggttc
     1441 attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa gcatctgtgc
     1501 ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca aagaatctga
     1561 gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa cgaagaatct
     1621 gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc aaacaaagaa
     1681 tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt accaacaaag
     1741 aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt tttctaacaa
     1801 agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct cttgataact
     1861 ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta ttttctcttc
     1921 cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag ctgcgggtgc
     1981 attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat tgcgcatact
     2041 ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt atgaacggtt
     2101 tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg tattgttttc
     2161 gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa tactagagat
     2221 aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa aggtggatgg
     2281 gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt tgagcaatgt
     2341 ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc gtttttggtt
     2401 ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa gttcctatac
     2461 tttctagcta gagaatagga acttcggaat aggaacttca aagcgtttcc gaaaacgagc
     2521 gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt cgcacctata
     2581 tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg tgtttatgct
     2641 taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag tacctcctgt
     2701 gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc ctttagctgt
     2761 tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat catttccttt
     2821 gatattggat cgatccgatg ataagctgtc aaacatgaga attgatcttt tatgcttgct
     2881 tttcaaaagg cttgcaggca agtgcacaaa caatacttaa ataaatacta ctcagtaata
     2941 acctatttct tagcattttt gacgaaattt gctattttgt tagagtcttt tacaccattt
     3001 gtctccacac ctccgcttac atcaacacca ataacgccat ttaatctaag cgcatcacca
     3061 acattttctg gcgtcagtcc accagctaac ataaaatgta agctctcggg gctctcttgc
     3121 cttccaaccc agtcagaaat cgagttccaa tccaaaagtt cacctgtccc acctgcttct
     3181 gaatcaaaca agggaataaa cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg
     3241 cagtcttttg gaaatacgag tcttttaata actggcaaac cgaggaactc ttggtattct
     3301 tgccacgact catctccatg cagttggacg atatcaatgc cgtaatcatt gaccagagcc
     3361 aaaacatcct ccttaggttg attacgaaac acgccaacca agtatttcgg agtgcctgaa
     3421 ctatttttat atgcttttac aagacttgaa attttccttg caataaccgg gtcaattgtt
     3481 ctctttctat tgggcacaca tataataccc agcaagtcag catcggaatc tagtgcacat
     3541 tctgcggcct ctgtgctctg caagccgcaa actttcacca atggaccaga actacctgtg
     3601 aaattaataa cagacatact ccaagctgcc tttgtgtgct taatcacgta tactcacgtg
     3661 ctcaatagtc accaatgccc tccctcttgg ccctctcctt ttcttttttc gaccgaatta
     3721 attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa
     3781 taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     3841 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     3901 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     3961 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     4021 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     4081 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     4141 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     4201 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     4261 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     4321 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     4381 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     4441 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     4501 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     4561 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     4621 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     4681 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     4741 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     4801 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     4861 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     4921 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     4981 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     5041 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     5101 atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     5161 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     5221 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     5281 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     5341 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     5401 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     5461 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     5521 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     5581 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     5641 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     5701 gcagcgagtc agtgagcgag gaagcggaag a
//
