LOCUS       40924_49707        5586 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector yGALset401, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5586)
  AUTHORS   Enomoto S, Chen G, Berman J.
  TITLE     Vectors for expressing T7 epitope- and His6 affinity-tagged fusion 
            proteins in S. cerevisiae
  JOURNAL   BioTechniques 24 (5), 782-786 (1998)
  PUBMED    9591127
REFERENCE   2  (bases 1 to 5586)
  AUTHORS   Enomoto S, Berman J.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-JAN-1998) Plant Biology, University of Minnesota, 220 
            Biological Sciences Center, St. Paul, MN 55108, USA
REFERENCE   3  (bases 1 to 5586)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5586)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "BioTechniques"; date: "1998"; volume: "24"; issue: "5"; pages: 
            "782-786"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-JAN-1998) Plant Biology, University of Minnesota, 220 Biological
            Sciences Center, St. Paul, MN 55108, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        70..88
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(121..785)
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
     RBS             804..826
                     /label=RBS
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             846..863
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             867..899
                     /codon_start=1
                     /label=T7 tag (gene 10 leader)
                     /note="leader peptide from bacteriophage T7 gene 10"
                     /translation="MASMTGGQQMG"
     CDS             903..926
                     /codon_start=1
                     /label=Xpress(TM) tag
                     /note="Xpress(TM) epitope tag, including an enterokinase 
                     recognition and cleavage site"
                     /translation="DLYDDDDK"
     misc_feature    926..972
                     /label=multiple cloning sites
                     /note="multiple cloning sites"
     terminator      1036..1083
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    complement(1797..2959)
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN4 centromere fused to the
                     autonomously replicating sequence ARS1/ARS416"
     promoter        complement(3473..3574)
                     /label=TRP1 promoter
     promoter        3680..3784
                     /label=AmpR promoter
     CDS             3785..4642
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4816..5404
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gatctcgatc
       61 ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct aggatccggg
      121 gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt
      181 attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt
      241 tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta
      301 attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg
      361 attcgttcat ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca
      421 taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc
      481 gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg
      541 ctgtcacccg ctcggcggct tctaatccgt acttcaatat agcaatgagc agttaagcgt
      601 attactgaaa gttccaaaga gaaggttttt ttaggctaag ataatggggc tctttacatt
      661 tccacaacat ataagtaaga ttagatatgg atatgtatat ggatatgtat atggtggtaa
      721 tgccatgtaa tatgattatt aaacttcttt gcgtccatcc aaaaaaaaag taagaatttt
      781 tgaaaattcg aattctagat aattttgttt aactttaaga aggagatata catatgcggg
      841 gttctcatca tcatcatcat catggtatgg ctagcatgac tggtggacag caaatgggtc
      901 gggatctgta cgacgatgac gataaggatc cgagctcgag atctgcagct ggtaccatgg
      961 aattcgaagc ttgatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc
     1021 accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
     1081 ttgctgaaag gaggaactat atccggatct ggcgtaatag cgaagaggcc cgcaccgatc
     1141 gcccttccca acagttgcgc agcctgaatg gcgaatggcg cctgatgcgg tattttctcc
     1201 ttacgcatct gtgcggtatt tcacaccgca tatatcgctg ggccattctc atgaagaata
     1261 tcttgaattt attgtcatat tactagttgg tgtggaagtc catatatcgg tgatcaatat
     1321 agtggttgac atgctggcta gtcaacattg agccttttga tcatgcaaat atattacggt
     1381 attttacaat caaatatcaa acttaactat tgactttata acttatttag gtggtaacat
     1441 tcttataaaa aagaaaaaaa ttactgcaaa acagtactag cttttaactt gtatcctagg
     1501 ttatctatgc tgtctcacca tagagaatat tacctatttc agaatgtatg tccatgattc
     1561 gccgggtaaa tacatataat acacaaatct ggcttaataa agtctataat atatctcata
     1621 aagaagtgct aaattggcta gtgctatata tttttaagaa aatttctttt gactaagtcc
     1681 atatcgactt tgtaaaagtt cactttagca tacatatatt acacgagcca gaaattgtaa
     1741 cttttgccta aaatcacaaa ttgcaaaatt taattgcttg caaaaggtca catgcttata
     1801 atcaactttt ttaaaaattt aaaatacttt tttatttttt atttttaaac ataaatgaaa
     1861 taatttattt attgtttatg attaccgaaa cataaaacct gctcaagaaa aagaaactgt
     1921 tttgtccttg gaaaaaaagc actacctagg agcggccaaa atgccgaggc tttcatagct
     1981 taaactcttt acagaaaata ggcattatag atcagttcga gttttcttat tcttccttcc
     2041 ggttttatcg tcacagtttt acagtaaata agtatcacct cttagagttc gatgataagc
     2101 tgtcaaacat gagaattaat tccacatgtt aaaatagtga aggagcatgt tcggcacaca
     2161 gtggaccgaa cgtggggtaa gtgcactagg gtccggttaa acggatctcg cattgatgag
     2221 gcaacgctaa ttatcaacat atagattgtt atctatctgc atgaacacga aatctttact
     2281 tgacgacttg aggctgatgg tgtttatgca aagaaaccac tgtgtttaat atgtgtcact
     2341 gtttgatatt actgtcagcg tagaagataa tagtaaaagc ggttaataag tgtatttgag
     2401 ataagtgtga taaagttttt acagcgaaaa gacgataaat acaagaaaat gattacgagg
     2461 atacggagag aggtatgtac atgtgtattt atatactaag ctgccggcgg ttgtttgcaa
     2521 gaccgagaaa aggctagcaa gaatcgggtc attgtagcgt atgcgcctgt gaacattctc
     2581 ttcaacaagt ttgattccat tgcggtgaaa tggtaaaagt caaccccctg cgatgtatat
     2641 tttcctgtac aatcaatcaa aaagccaaat gatttagcat tatctttaca tcttgttatt
     2701 ttacagattt tatgtttaga tcttttatgc ttgcttttca aaaggcttgc aggcaagtgc
     2761 acaaacaata cttaaataaa tactactcag taataaccta tttcttagca tttttgacga
     2821 aatttgctat tttgttagag tcttttacac catttgtctc cacacctccg cttacatcaa
     2881 caccaataac gccatttaat ctaagcgcat caccaacatt ttctggcgtc agtccaccag
     2941 ctaacataaa atgtaagctc tcggggctct cttgccttcc aacccagtca gaaatcgagt
     3001 tccaatccaa aagttcacct gtcccacctg cttctgaatc aaacaaggga ataaacgaat
     3061 gaggtttctg tgaagctgca ctgagtagta tgttgcagtc ttttggaaat acgagtcttt
     3121 taataactgg caaaccgagg aactcttggt attcttgcca cgactcatct ccatgcagtt
     3181 ggacgatatc aatgccgtaa tcattgacca gagccaaaac atcctcctta ggttgattac
     3241 gaaacacgcc aaccaagtat ttcggagtgc ctgaactatt tttatatgct tttacaagac
     3301 ttgaaatttt ccttgcaata accgggtcaa ttgttctctt tctattgggc acacatataa
     3361 tacccagcaa gtcagcatcg gaatctagtg cacattctgc ggcctctgtg ctctgcaagc
     3421 cgcaaacttt caccaatgga ccagaactac ctgtgaaatt aataacagac atactccaag
     3481 ctgcctttgt gtgcttaatc acgtatactc acgtgctcaa tagtcaccaa tgccctccct
     3541 cttggccctc tccttttctt ttttcgaccg aattaattct tgaagacgaa agggcctcgt
     3601 gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg
     3661 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     3721 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     3781 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     3841 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     3901 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     3961 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     4021 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     4081 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     4141 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     4201 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     4261 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     4321 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     4381 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     4441 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     4501 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     4561 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     4621 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     4681 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     4741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     4801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     4861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     4921 gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta
     4981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     5041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     5101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     5161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     5221 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     5281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     5341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     5401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca
     5461 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg
     5521 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc
     5581 ggaaga
//
