LOCUS       40924_49372       14656 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector w-repeats, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14656)
  AUTHORS   Akmammedov A, Geigges M, Paro R.
  TITLE     Single vector non-leaky gene expression system for Drosophila 
            melanogaster
  JOURNAL   Sci Rep 7 (1), 6899 (2017)
  PUBMED    28761084
REFERENCE   2  (bases 1 to 14656)
  AUTHORS   Akmammedov A, Geigges M, Paro R.
  TITLE     Direct Submission
  JOURNAL   Submitted (04-MAY-2017) Biosystem Science and Engineering, ETH 
            Zurich, Mattenstrasse 26, Basel 4058, Switzerland
REFERENCE   3  (bases 1 to 14656)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 14656)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep"; 
            date: "2017"; volume: "7"; issue: "1"; pages: "6899"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (04-MAY-2017) Biosystem Science and Engineering, ETH Zurich, 
            Mattenstrasse 26, Basel 4058, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..14656
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     protein_bind    4842..5227
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     polyA_signal    complement(5235..5661)
                     /label=hsp70 poly(A)
                     /note="polyadenlyation signal from the Drosophila
                     melanogaster hsp70Ab gene"
     CDS             complement(5680..8724)
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     promoter        complement(8846..9294)
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     misc_feature    complement(9307..11747)
                     /label=w-repeats
                     /note="w-repeats"
     protein_bind    11755..12140
                     /label=gypsy insulator
                     /note="chromatin insulator from Drosophila"
     protein_bind    12180..12249
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(12468..12486)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12507..12523)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    12531..12547
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(12555..12585)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(12600..12621)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(12909..13497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(13671..14528)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(14529..14633)
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagagc ttaggattta aatgcttgca tgcctgcagg tcacgtaata agtgtgcgtt
     4861 gaatttattc gcaaaaacat tgcatatttt cggcaaagta aaattttgtt gcatacctta
     4921 tcaaaaaata agtgctgcat actttttaga gaaaccaaat aattttttat tgcatacccg
     4981 tttttaataa aatacattgc ataccctctt ttaataaaaa atattgcata ctttgacgaa
     5041 acaaattttc gttgcatacc caataaaaga ttattatatt gcatacccgt ttttaataaa
     5101 atacattgca taccctcttt taataaaaaa tattgcatac gttgacgaaa caaattttcg
     5161 ttgcataccc aataaaagat tattatattg catacctttt cttgccatac catttagccg
     5221 atcaattgcg gccgctcctg accgtccatc gcaataaaat gagccattat gagatcgaaa
     5281 gggtctacga aaatccgttc ggaaaaaatc agaaaatcat caaagccgaa tataattaaa
     5341 atgtattact agctaaagaa atcatcacta atatagaatg tagaatgaac catgtatata
     5401 gatactaatg tatcgtaaga ctttcaaaag tctacaagac attaaatgac aagttgactt
     5461 taaatttcaa ataaataatt tattttttct ataagcaata acatttttgc taaattaaga
     5521 cttggtaatt aggtaatact attgttgttc tatggaatat tcgatcgaaa cattcttatc
     5581 agtctcaaaa acttaaaaca aacttataat ataacccata tgttataacc cattgatgaa
     5641 caaaaattag actctttggc cttagtcgac ggatccttat ttttgacacc agaccaactg
     5701 gtaatggtag cgaccggcgc tcagctgtaa ttccgccgat actgacgggc tccaggagtc
     5761 gtcgccacca atccccatat ggaaaccgtc gatattcagc catgtgcctt cttccgcgtg
     5821 cagcagatgg cgatggctgg tttccatcag ttgctgttga ctgtagcggc tgatgttgaa
     5881 ctggaagtcg ccgcgccact ggtgtgggcc ataattcaat tcgcgcgtcc cgcagcgcag
     5941 accgttttcg ctcgggaaga cgtacggggt atacatgtct gacaatggca gatcccagcg
     6001 gtcaaaacag gcggcagtaa ggcggtcggg atagttttct tgcggcccta atccgagcca
     6061 gtttacccgc tctgctacct gcgccagctg gcagttcagg ccaatccgcg ccggatgcgg
     6121 tgtatcgctc gccacttcaa catcaacggt aatcgccatt tgaccactac catcaatccg
     6181 gtaggttttc cggctgataa ataaggtttt cccctgatgc tgccacgcgt gagcggtcgt
     6241 aatcagcacc gcatcagcaa gtgtatctgc cgtgcactgc aacaacgctg cttcggcctg
     6301 gtaatggccc gccgccttcc agcgttcgac ccaggcgtta gggtcaatgc gggtcgcttc
     6361 acttacgcca atgtcgttat ccagcggtgc acgggtgaac tgatcgcgca gcggcgtcag
     6421 cagttgtttt ttatcgccaa tccacatctg tgaaagaaag cctgactggc ggttaaattg
     6481 ccaacgctta ttacccagct cgatgcaaaa atccatttcg ctggtggtca gatgcgggat
     6541 ggcgtgggac gcggcgggga gcgtcacact gaggttttcc gccagacgcc actgctgcca
     6601 ggcgctgatg tgcccggctt ctgaccatgc ggtcgcgttc ggttgcacta cgcgtactgt
     6661 gagccagagt tgcccggcgc tctccggctg cggtagttca ggcagttcaa tcaactgttt
     6721 accttgtgga gcgacatcca gaggcacttc accgcttgcc agcggcttac catccagcgc
     6781 caccatccag tgcaggagct cgttatcgct atgacggaac aggtattcgc tggtcacttc
     6841 gatggtttgc ccggataaac ggaactggaa aaactgctgc tggtgttttg cttccgtcag
     6901 cgctggatgc ggcgtgcggt cggcaaagac cagaccgttc atacagaact ggcgatcgtt
     6961 cggcgtatcg ccaaaatcac cgccgtaagc cgaccacggg ttgccgtttt catcatattt
     7021 aatcagcgac tgatccaccc agtcccagac gaagccgccc tgtaaacggg gatactgacg
     7081 aaacgcctgc cagtatttag cgaaaccgcc aagactgtta cccatcgcgt gggcgtattc
     7141 gcaaaggatc agcgggcgcg tctctccagg tagcgaaagc cattttttga tggaccattt
     7201 cggcacagcc gggaagggct ggtcttcatc cacgcgcgcg tacatcgggc aaataatatc
     7261 ggtggccgtg gtgtcggctc cgccgccttc atactgcacc gggcgggaag gatcgacaga
     7321 tttgatccag cgatacagcg cgtcgtgatt agcgccgtgg cctgattcat tccccagcga
     7381 ccagatgatc acactcgggt gattacgatc gcgctgcacc attcgcgtta cgcgttcgct
     7441 catcgccggt agccagcgcg gatcatcggt cagacgattc attggcacca tgccgtgggt
     7501 ttcaatattg gcttcatcca ccacatacag gccgtagcgg tcgcacagcg tgtaccacag
     7561 cggatggttc ggataatgcg aacagcgcac ggcgttaaag ttgttctgct tcatcagcag
     7621 gatatcctgc accatcgtct gctcatccat gacctgacca tgcagaggat gatgctcgtg
     7681 acggttaacg cctcgaatca gcaacggctt gccgttcagc agcagcagac cattttcaat
     7741 ccgcacctcg cggaaaccga catcgcaggc ttctgcttca atcagcgtgc cgtcggcggt
     7801 gtgcagttca accaccgcac gatagagatt cgggatttcg gcgctccaca gtttcgggtt
     7861 ttcgacgttc agacgtagtg tgacgcgatc ggcataacca ccacgctcat cgataatttc
     7921 accgccgaaa ggcgcggtgc cgctggcgac ctgcgtttca ccctgccata aagaaactgt
     7981 tacccgtagg tagtcacgca actcgccgca catctgaact tcagcctcca gtacagcgcg
     8041 gctgaaatca tcattaaagc gagtggcaac atggaaatcg ctgatttgtg tagtcggttt
     8101 atgcagcaac gagacgtcac ggaaaatgcc gctcatccgc cacatatcct gatcttccag
     8161 ataactgccg tcactccagc gcagcaccat caccgcgagg cggttttctc cggcgcgtaa
     8221 aaatgcgctc aggtcaaatt cagacggcaa acgactgtcc tggccgtaac cgacccagcg
     8281 cccgttgcac cacagatgaa acgccgagtt aacgccatca aaaataattc gcgtctggcc
     8341 ttcctgtagc cagctttcat caacattaaa tgtgagcgag taacaacccg tcggattctc
     8401 cgtgggaaca aacggcggat tgaccgtaat gggataggtc acgttggtgt agatgggcgc
     8461 atcgtaaccg tgcatctgcc agtttgaggg gacgacgaca gtatcggcct caggaagatc
     8521 gcactccagc cagctttccg gcaccgcttc tggtgccgga aaccaggcaa agcgccattc
     8581 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     8641 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
     8701 ccagtcacga cgttgtaaaa cgacgggatc gcgcttgagc agctccttgc tggtgtccag
     8761 accaatgcct cccagaccgg caacgaaaat cacgttcttg ttggtcaaag taaacgacat
     8821 ggtggcgaat tcccaattcc ctattcagag ttctcttctt gtattcaata attacttctt
     8881 ggcagatttc agtagttgca gttgatttac ttggttgctg gttactttta attgattcac
     8941 tttaacttgc actttactgc agattgttta gcttgttcag ctgcgcttgt ttatttgctt
     9001 agctttcgct tagcgacgtg ttcactttgc ttgtttgaat tgaattgtcg ctccgtagac
     9061 gaagcgctct atttatactc cggcgctctt ttcgcgaaca ttcgaggcgc gctctctcga
     9121 accaacgaga gcagtatgcc gtttactgtg tgacagagtg agagagcatt agtgcagaga
     9181 gggagaccca aaaagaaaag agagaataac gaataacggc cagagaaatt tctcgagttt
     9241 tcttctgcca aacaaatgac ctaccacaat aaccagtttg ttttgggatt ctagagatct
     9301 gctagctcaa actgcggtcc atgtttatat agagcgtgac accacgatgc ctcgttgcag
     9361 aatagtccgc gtgtccggtt gaccagctgc cgccatccgg agcccggctg attgaccgcc
     9421 ccaaagatgt ccatattgtg ccaggcatag gtgaggttct cggctagttg gccgctccct
     9481 gaaccggagt cctccggcgg actgggtggc cggagcgtgc cgtagttttt ggcctgcccg
     9541 aagccctggt tgatcgttgg gaaccggagg gcaaaatgtc gggcaacgat gcctgtagca
     9601 atggcactag ctcaaactgc ggtccatgtt tatatagagc gtgacaccac gatgcctcgt
     9661 tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc ggctgattga
     9721 ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct agttggccgc
     9781 tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag tttttggcct
     9841 gcccgaagcc ctggttgatc gttgggaacc ggagggcaaa atgtcgggca acgatgcctg
     9901 tagcaatggc actagctcaa actgcggtcc atgtttatat agagcgtgac accacgatgc
     9961 ctcgttgcag aatagtccgc gtgtccggtt gaccagctgc cgccatccgg agcccggctg
    10021 attgaccgcc ccaaagatgt ccatattgtg ccaggcatag gtgaggttct cggctagttg
    10081 gccgctccct gaaccggagt cctccggcgg actgggtggc cggagcgtgc cgtagttttt
    10141 ggcctgcccg aagccctggt tgatcgttgg gaaccggagg gcaaaatgtc gggcaacgat
    10201 gcctgtagca atggcactag ctcaaactgc ggtccatgtt tatatagagc gtgacaccac
    10261 gatgcctcgt tgcagaatag tccgcgtgtc cggttgacca gctgccgcca tccggagccc
    10321 ggctgattga ccgccccaaa gatgtccata ttgtgccagg cataggtgag gttctcggct
    10381 agttggccgc tccctgaacc ggagtcctcc ggcggactgg gtggccggag cgtgccgtag
    10441 tttttggcct gcccgaagcc ctggttgatc gttgggaacc ggagggcaaa atgtcgggca
    10501 acgatgcctg tagcaatggc actagctcaa actgcggtcc atgtttatat agagcgtgac
    10561 accacgatgc ctcgttgcag aatagtccgc gtgtccggtt gaccagctgc cgccatccgg
    10621 agcccggctg attgaccgcc ccaaagatgt ccatattgtg ccaggcatag gtgaggttct
    10681 cggctagttg gccgctccct gaaccggagt cctccggcgg actgggtggc cggagcgtgc
    10741 cgtagttttt ggcctgcccg aagccctggt tgatcgttgg gaaccggagg gcaaaatgtc
    10801 gggcaacgat gcctgtagca atggcactag ctcaaactgc ggtccatgtt tatatagagc
    10861 gtgacaccac gatgcctcgt tgcagaatag tccgcgtgtc cggttgacca gctgccgcca
    10921 tccggagccc ggctgattga ccgccccaaa gatgtccata ttgtgccagg cataggtgag
    10981 gttctcggct agttggccgc tccctgaacc ggagtcctcc ggcggactgg gtggccggag
    11041 cgtgccgtag tttttggcct gcccgaagcc ctggttgatc gttgggaacc ggagggcaaa
    11101 atgtcgggca acgatgcctg tagcaatggc actagctcaa actgcggtcc atgtttatat
    11161 agagcgtgac accacgatgc ctcgttgcag aatagtccgc gtgtccggtt gaccagctgc
    11221 cgccatccgg agcccggctg attgaccgcc ccaaagatgt ccatattgtg ccaggcatag
    11281 gtgaggttct cggctagttg gccgctccct gaaccggagt cctccggcgg actgggtggc
    11341 cggagcgtgc cgtagttttt ggcctgcccg aagccctggt tgatcgttgg gaaccggagg
    11401 gcaaaatgtc gggcaacgat gcctgtagca atggcactag ctcaaactgc ggtccatgtt
    11461 tatatagagc gtgacaccac gatgcctcgt tgcagaatag tccgcgtgtc cggttgacca
    11521 gctgccgcca tccggagccc ggctgattga ccgccccaaa gatgtccata ttgtgccagg
    11581 cataggtgag gttctcggct agttggccgc tccctgaacc ggagtcctcc ggcggactgg
    11641 gtggccggag cgtgccgtag tttttggcct gcccgaagcc ctggttgatc gttgggaacc
    11701 ggagggcaaa atgtcgggca acgatgcctg tagcaatggc actagtaggt acctcacgta
    11761 ataagtgtgc gttgaattta ttcgcaaaaa cattgcatat tttcggcaaa gtaaaatttt
    11821 gttgcatacc ttatcaaaaa ataagtgctg catacttttt agagaaacca aataattttt
    11881 tattgcatac ccgtttttaa taaaatacat tgcataccct cttttaataa aaaatattgc
    11941 atactttgac gaaacaaatt ttcgttgcat acccaataaa agattattat attgcatacc
    12001 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tacgttgacg
    12061 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcatacct tttcttgcca
    12121 taccatttag ccgatcaatt aagcttagat ccactagtgt cgacgatgta ggtcacggtc
    12181 tcgaagccgc ggtgcgggtg ccagggcgtg cccttgggct ccccgggcgc gtactccacc
    12241 tcacccatct ggtccatcat gatgaacggg tcgaggtggc ggtagttgat cccggcgaac
    12301 gcgcggcgca ccgggaagcc ctcgccctcg aaaccgctgg gcgcggtggt cacggtgagc
    12361 acgggacgtg cgacggcgtc ggcgggtgcg gatacgcggg gcagcgtcag cgggttctcg
    12421 acggtcacgg cgggcatgtc gacactagtt ctagccagct tttgttccct ttagtgaggg
    12481 ttaatttcga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    12541 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
    12601 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    12661 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    12721 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    12781 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    12841 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    12901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    12961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    13021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    13081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    13141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    13201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    13261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    13321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    13381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    13441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    13501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    13561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    13621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    13681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    13741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    13801 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    13861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    13921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    13981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    14041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    14101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    14161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    14221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    14281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    14341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    14401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    14461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    14521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    14581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    14641 ccccgaaaag tgccac
//
