LOCUS       V006699                 7084 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V006699
VERSION     V006699
KEYWORDS    shYAP1 # 1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7084)
  AUTHORS   Rosenbluh J, Nijhawan D, Cox AG, Li X, Neal JT, Schafer EJ, Zack TI,
            Wang X, Tsherniak A, Schinzel AC, Shao DD, Schumacher SE, Weir BA,
            Vazquez F, Cowley GS, Root DE, Mesirov JP, Beroukhim R, Kuo CJ,
            Goessling W, Hahn WC
  TITLE     beta-Catenin-Driven Cancers Require a YAP1 Transcriptional Complex
            for Survival and Tumorigenesis.
  JOURNAL   Cell. 2012 Dec 21;151(7):1457-73. doi: 10.1016/j.cell.2012.11.026.
            Epub 2012 Dec 13.
   PUBMED   23245941
REFERENCE   2  (bases 1 to 7084)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 7084)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; doi:
            "10.1016/j.cell.2012.11.026"; journalName: "Cell"; date:
            "2012-12-21- 21"; volume: "151"; issue: "7"; pages: "1457-73"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7084
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..17
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     25..41
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     promoter        62..80
                     /label="T3 promoter"
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        108..334
                     /label="RSV promoter"
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             335..515
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    562..687
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1180..1413
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1598..1642
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1791..1832
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     promoter        1940..2180
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_feature    2285..2402
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2451..2961
                     /label="hPGK promoter"
                     /note="human phosphoglycerate kinase 1 promoter"
     CDS             2983..3579
                     /label="PuroR"
                     /note="puromycin N-acetyltransferase"
     LTR             3710..3943
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4015..4149
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      4176..4311
                     /label="SV40 ori"
                     /note="SV40 origin of replication"
     promoter        complement(4332..4350)
                     /label="T7 promoter"
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4360..4376)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      4518..4973
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        4999..5103
                     /label="AmpR promoter"
     CDS             5104..5961
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      6135..6723
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     6877..6894
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     protein_bind    7011..7032
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7047..7077
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
       61 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
      121 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
      181 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
      241 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
      301 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
      361 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      421 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      481 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      541 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      601 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      661 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      721 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      781 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      841 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
      901 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
      961 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1021 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     1081 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     1141 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     1201 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     1261 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     1321 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     1381 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     1441 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
     1501 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
     1561 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
     1621 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
     1681 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
     1741 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
     1801 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
     1861 agacagatcc attcgattag tgaacggatc tcgacggtat cgatcacgag actagcctcg
     1921 agcggccgcc cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg
     1981 atacaaggct gttagagaga taattggaat taatttgact gtaaacacaa agatattagt
     2041 acaaaatacg tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg
     2101 ttttaaaatg gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt
     2161 atatatcttg tggaaaggac gaaacaccgg gccaccaagc tagataaaga actcgagttc
     2221 tttatctagc ttggtggctt tttgaattct cgacctcgag acaaatggca gtattcatcc
     2281 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     2341 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     2401 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag ggggttgggg
     2461 ttgcgccttt tccaaggcag ccctgggttt gcgcagggac gcggctgctc tgggcgtggt
     2521 tccgggaaac gcagcggcgc cgaccctggg tctcgcacat tcttcacgtc cgttcgcagc
     2581 gtcacccgga tcttcgccgc tacccttgtg ggccccccgg cgacgcttcc tgctccgccc
     2641 ctaagtcggg aaggttcctt gcggttcgcg gcgtgccgga cgtgacaaac ggaagccgca
     2701 cgtctcacta gtaccctcgc agacggacag cgccagggag caatggcagc gcgccgaccg
     2761 cgatgggctg tggccaatag cggctgctca gcagggcgcg ccgagagcag cggccgggaa
     2821 ggggcggtgc gggaggcggg gtgtggggcg gtagtgtggg ccctgttcct gcccgcgcgg
     2881 tgttccgcat tctgcaagcc tccggagcgc acgtcggcag tcggctccct cgttgaccga
     2941 atcaccgacc tctctcccca gggggatcca ccggagctta ccatgaccga gtacaagccc
     3001 acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg
     3061 ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc
     3121 accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc
     3181 gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg
     3241 gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag
     3301 caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc
     3361 accgtcggcg tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc
     3421 ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc
     3481 aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa
     3541 ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gacgcccgcc ccacgacccg
     3601 cagcgcccga ccgaaaggag cgcacgaccc catgcatcgg tacctttaag accaatgact
     3661 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     3721 attcactccc aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac
     3781 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     3841 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     3901 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     3961 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     4021 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     4081 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     4141 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     4201 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     4261 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     4321 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     4381 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     4441 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     4501 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     4561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     4621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     4681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     4741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     4801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     4861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     4921 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     4981 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     5041 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     5101 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     5161 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     5221 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     5281 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     5341 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     5401 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     5461 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     5521 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     5581 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     5641 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     5701 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     5761 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     5821 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     5881 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     5941 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     6001 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     6061 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     6121 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     6181 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     6241 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     6301 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     6361 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     6421 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     6481 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     6541 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     6601 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     6661 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     6721 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     6781 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     6841 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     6901 gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc
     6961 tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt
     7021 tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt
     7081 ggaa
//
