LOCUS       40924_48768        5899 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Insertion vector scAAV-CMV-GFP, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5899)
  AUTHORS   Rosas LE, Grieves JL, Zaraspe K, La Perle KM, Fu H, McCarty DM.
  TITLE     Patterns of scAAV Vector Insertion Associated With Oncogenic Events 
            in a Mouse Model for Genotoxicity
  JOURNAL   Mol. Ther. (2012) In press
  PUBMED    22990674
REFERENCE   2  (bases 1 to 5899)
  AUTHORS   Rosas LE, Grieves JL, Zaraspe K, La Perle KMD., Fu H, McCarty DM.
  TITLE     Direct Submission
  JOURNAL   Submitted (01-AUG-2012) Pediatrics/Center for Gene Therapy, Research
            Institute at Nationwide Children's Hospital, 700 Children's Dr, 
            Columbus, OH 43205, USA
REFERENCE   3  (bases 1 to 5899)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 5899)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol. Ther. 
            (2012) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (01-AUG-2012) Pediatrics/Center for Gene Therapy, Research Institute
            at Nationwide Children's Hospital, 700 Children's Dr, Columbus, OH 
            43205, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..112
                     /label=AAV2 terminal repeat
                     /note="AAV2 terminal repeat"
     polyA_signal    complement(202..313)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     polyA_signal    405..539
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(555..1271)
                     /codon_start=1
                     /label=EGFP
                     /note="enhanced GFP"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     intron          complement(1348..1444)
                     /label=SV40 intron
                     /note="modified SV40 intron with splice donor and acceptor 
                     sites"
     promoter        complement(1582..1785)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     enhancer        complement(1786..2069)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer;
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     repeat_region   2136..2272
                     /label=AAV2 terminal repeat
                     /note="AAV2 terminal repeat"
     rep_origin      2683..3196
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3942..4046
                     /label=AmpR promoter
     CDS             4047..4904
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5078..5666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cagcagctgc gcgctcgctc gctcactgag gccgcccggg caaagcccgg gcgtcgggcg
       61 acctttggtc gcccggcctc agtgagcgag cgagcgcgca gagagggagt ggggttcctg
      121 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa
      181 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga
      241 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa
      301 ctagaaggca cagtcgaggc tgatcagcga gctctagtcg actaaaaaac ctcccacacc
      361 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
      421 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      481 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
      541 ccgcggccgc tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg
      601 aactccagca ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg
      661 gtgctcaggt agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc
      721 tggtagtggt cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc
      781 accttgatgc cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg
      841 tactccagct tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg
      901 atgcggttca ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg
      961 tcgtccttga agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag
     1021 aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg
     1081 gtggtcacga gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg
     1141 gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg
     1201 tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc
     1261 ttgctcacca tggtggcgac cggtggatcc cgggccgcgg gtacaattcc gcagctttta
     1321 gagcagaagt aacacttccg tacaggccta gaagtaaagg caacatccac tgaggagcag
     1381 ttctttgatt tgcaccacca ccggatccgg gacctgaaat aaaagacaaa aagactaaac
     1441 ttaccagtta actttctggt ttttcagttc ctcgagtacc ggatcctcta gagtccggag
     1501 gctggatcgg tcccggtgtc ttctatggag gtcaaaacag cgtggatggc gtctccaggc
     1561 gatctgacgg ttcactaaac gagctctgct tatatagacc tcccaccgta cacgcctacc
     1621 gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc gttgattttg
     1681 gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc
     1741 aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg taatagcgat
     1801 gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata
     1861 atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata
     1921 cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg
     1981 gaaagtccct attggtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     2041 gcgggccatt taccgtaagt tatgtaacgg gtaccgaatt cagatcgagc atggctacgt
     2101 agataagtag catggcgggt taatcattaa ctacaaggaa cccctagtga tggagttggc
     2161 cactccctct ctgcgcgctc gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg
     2221 cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg cgccagctgg cgtaatagcg
     2281 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggaatt
     2341 ccagacgatt gagcgtcaaa atgtaggtat ttccatgagc gtttttcctg ttgcaatggc
     2401 tggcggtaat attgttctgg atattaccag caaggccgat agtttgagtt cttctactca
     2461 ggcaagtgat gttattacta atcaaagaag tattgcgaca acggttaatt tgcgtgatgg
     2521 acagactctt ttactcggtg gcctcactga ttataaaaac acttctcagg attctggcgt
     2581 accgttcctg tctaaaatcc ctttaatcgg cctcctgttt agctcccgct ctgattctaa
     2641 cgaggaaagc acgttatacg tgctcgtcaa agcaaccata gtacgcgccc tgtagcggcg
     2701 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     2761 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     2821 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     2881 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     2941 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3001 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     3061 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3121 tattaacgtt tacaatttaa atatttgctt atacaatctt cctgtttttg gggcttttct
     3181 gattatcaac cggggtacat atgattgaca tgctagtttt acgattaccg ttcatcgatt
     3241 ctcttgtttg ctccagactc tcaggcaatg acctgatagc ctttgtagag acctctcaaa
     3301 aatagctacc ctctccggca tgaatttatc agctagaacg gttgaatatc atattgatgg
     3361 tgatttgact gtctccggcc tttctcaccc gtttgaatct ttacctacac attactcagg
     3421 cattgcattt aaaatatatg agggttctaa aaatttttat ccttgcgttg aaataaaggc
     3481 ttctcccgca aaagtattac agggtcataa tgtttttggt acaaccgatt tagctttatg
     3541 ctctgaggct ttattgctta attttgctaa ttctttgcct tgcctgtatg atttattgga
     3601 tgttggaatt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
     3661 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     3721 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     3781 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     3841 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     3901 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5221 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5881 gttggccgat tcattaatg
//
