LOCUS       Exported                9791 bp DNA     circular SYN 20-JUL-2025
DEFINITION  Exported.
ACCESSION   V017119
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9791)
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 9791)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..9791
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..633
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    680..805
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1302..1535
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1720..1764
                     /label=gp41 peptide
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et 
                     al., 2013)"
     CDS             1913..1954
                     /label=Protein Tat
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     misc_feature    2026..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2206..3379
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     CDS             3389..4132
                     /label=rtTA-Advanced
                     /note="improved tetracycline-controlled transactivator"
     promoter        4184..4683
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             4710..5732
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
     misc_feature    5759..6347
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             6554..7187
                     /label=5' LTR
                     /note="5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7315..7331)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7339..7355)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7363..7393)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(7408..7429)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(7717..8305)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8479..9336)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(9337..9441)
                     /label=AmpR promoter
     polyA_signal    9489..9623
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
       61 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
      121 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
      181 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
      241 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
      301 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
      361 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
      421 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
      481 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      541 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      601 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
      661 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
      721 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
      781 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
      841 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
      901 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
      961 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     1021 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     1081 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     1141 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     1201 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     1261 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     1321 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     1381 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     1441 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     1501 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     1561 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     1621 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     1681 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     1741 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     1801 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     1861 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     1921 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     1981 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta aaagaaaagg
     2041 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     2101 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     2161 cagcagagat ccagtttatc gatgaggccc tttcgtcttc actcgaggtg cccgtcagtg
     2221 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     2281 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     2341 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     2401 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     2461 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     2521 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     2581 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     2641 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     2701 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     2761 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     2821 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     2881 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     2941 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     3001 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     3061 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     3121 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     3181 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     3241 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     3301 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     3361 tccatttcag gtgtcgtgag gatccaccat gtctagactg gacaagagca aagtcataaa
     3421 cggcgctctg gaattactca atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc
     3481 tcaaaagctg ggagttgagc agcctaccct gtactggcac gtgaagaaca agcgggccct
     3541 gctcgatgcc ctgccaatcg agatgctgga caggcatcat acccacttct gccccctgga
     3601 aggcgagtca tggcaagact ttctgcggaa caacgccaag tcattccgct gtgctctcct
     3661 ctcacatcgc gacggggcta aagtgcatct cggcacccgc ccaacagaga aacagtacga
     3721 aaccctggaa aatcagctcg cgttcctgtg tcagcaaggc ttctccctgg agaacgcact
     3781 gtacgctctg tccgccgtgg gccactttac actgggctgc gtattggagg aacaggagca
     3841 tcaagtagca aaagaggaaa gagagacacc taccaccgat tctatgcccc cacttctgag
     3901 acaagcaatt gagctgttcg accggcaggg agccgaacct gccttccttt tcggcctgga
     3961 actaatcata tgtggcctgg agaaacagct aaagtgcgaa agcggcgggc cggccgacgc
     4021 ccttgacgat tttgacttag acatgctccc agccgatgcc cttgacgact ttgaccttga
     4081 tatgctgcct gctgacgctc ttgacgattt tgaccttgac atgctccccg ggtaaggatc
     4141 cgcggccgcg ccggctctag atcgcgaacg cgtgaattct accgggtagg ggaggcgctt
     4201 ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg gcgctacaca
     4261 agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc ggctccgttc
     4321 tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt cccccccgcc
     4381 ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac tagtctcgtg
     4441 cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag cggccaatag
     4501 cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc cgggggcggg
     4561 ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg cccggcattc
     4621 tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc tccgggcctt
     4681 tcgacctgca gcccaagctt cctaggacca tgaaaaagcc tgaactcacc gcgacgtctg
     4741 tcgagaagtt tctgatcgaa aagttcgaca gcgtctccga cctgatgcag ctctcggagg
     4801 gcgaagaatc tcgtgctttc agcttcgatg taggagggcg tggatatgtc ctgcgggtaa
     4861 atagctgcgc cgatggtttc tacaaagatc gttatgttta tcggcacttt gcatcggccg
     4921 cgctcccgat tccggaagtg cttgacattg gggaattcag cgagagcctg acctattgca
     4981 tctcccgccg tgcacagggt gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg
     5041 ttctgcagcc ggtcgcggag gccatggatg cgatcgctgc ggccgatctt agccagacga
     5101 gcgggttcgg cccattcgga ccgcaaggaa tcggtcaata cactacatgg cgtgatttca
     5161 tatgcgcgat tgctgatccc catgtgtatc actggcaaac tgtgatggac gacaccgtca
     5221 gtgcgtccgt cgcgcaggct ctcgatgagc tgatgctttg ggccgaggac tgccccgaag
     5281 tccggcacct cgtgcacgcg gatttcggct ccaacaatgt cctgacggac aatggccgca
     5341 taacagcggt cattgactgg agcgaggcga tgttcgggga ttcccaatac gaggtcgcca
     5401 acatcttctt ctggaggccg tggttggctt gtatggagca gcagacgcgc tacttcgagc
     5461 ggaggcatcc ggagcttgca ggatcgccgc ggctccgggc gtatatgctc cgcattggtc
     5521 ttgaccaact ctatcagagc ttggttgacg gcaatttcga tgatgcagct tgggcgcagg
     5581 gtcgatgcga cgcaatcgtc cgatccggag ccgggactgt cgggcgtaca caaatcgccc
     5641 gcagaagcgc ggccgtctgg accgatggct gtgtagaagt actcgccgat agtggaaacc
     5701 gacgccccag cactcgtccg agggcaaagg aatagcccgg gcggggcgcg tctggaacaa
     5761 tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc
     5821 ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat
     5881 ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg
     5941 gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg
     6001 ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat
     6061 tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt
     6121 gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc
     6181 ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa
     6241 tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg
     6301 ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg gaattaattc
     6361 tgcagtcgag acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat
     6421 gctgattgtg cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct
     6481 caggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     6541 gaaaagaggg gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg
     6601 tggatctacc acacacaagg ctacttccct gattagcaga actacacacc agggccaggg
     6661 gtcagatatc cactgacctt tggatggtgc tacaagctag taccagttga gccagataag
     6721 gtagaagagg ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg
     6781 atggatgacc cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat
     6841 cacgtggccc gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac
     6901 aagggacttt ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg
     6961 cgagccctca gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt
     7021 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7081 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7141 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7201 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7261 ttgacattgc tagcgtttac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     7321 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     7381 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     7441 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     7501 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     7561 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     7621 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     7681 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     7741 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7801 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7861 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7921 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7981 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     8041 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     8101 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     8161 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     8221 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     8281 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     8341 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     8401 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8461 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8521 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8581 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8641 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8701 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8761 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     8821 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     8881 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     8941 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9001 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9061 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9121 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9181 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9241 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9301 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9361 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     9421 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     9481 gggagatcaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     9541 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     9601 atgtatctta tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac
     9661 ttcccacccc ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct
     9721 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
     9781 acttagtagt t
//
