LOCUS 40924_48713 7798 bp DNA circular SYN 18-DEC-2018 DEFINITION Cloning vector rpSE937, complete sequence. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7798) AUTHORS Elledge SJ, Mulligan JT, Ramer SW, Spottswood M, Davis RW. TITLE Lambda YES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations JOURNAL Proc. Natl. Acad. Sci. U.S.A. 88 (5), 1731-1735 (1991) PUBMED 1848010 REFERENCE 2 (bases 1 to 7798) AUTHORS Kitts PA. TITLE CLONTECH Vectors On Disc version 1.3 JOURNAL Unpublished REFERENCE 3 (bases 1 to 7798) AUTHORS Kitts PA. TITLE Direct Submission JOURNAL Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA REFERENCE 4 (bases 1 to 7798) TITLE Direct Submission REFERENCE 5 (bases 1 to 7798) AUTHORS . TITLE Direct Submission COMMENT SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl. Acad. Sci. U.S.A."; date: "1991"; volume: "88"; issue: "5"; pages: "1731-1735" COMMENT SGRef: number: 2; type: "Journal Article"; journalName: "Unpublished" COMMENT SGRef: number: 3; type: "Journal Article"; journalName: "Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East Meadow Circle, Palo Alto, CA 94303, USA" COMMENT SGRef: number: 4; type: "Journal Article" COMMENT This sequence has been compiled from information in the sequence databases, published literature and other sources, together with partial sequences obtained by CLONTECH. Lambda YES-P and pSE937 are no longer available from CLONTECH and CLONTECH will not update or revise this sequence. FEATURES Location/Qualifiers source 1..7798 /mol_type="other DNA" /organism="synthetic DNA construct" protein_bind complement(66..82) /label=lac operator /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(90..120) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind complement(135..156) /label=CAP binding site /note="CAP binding activates transcription in the presence of cAMP." promoter 1390..1610 /label=URA3 promoter CDS 1611..2411 /codon_start=1 /label=URA3 /note="orotidine-5'-phosphate decarboxylase, required for uracil biosynthesis" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" misc_feature complement(3757..4049) /label=CEN4 /note="S. cerevisiae CEN4 centromere" protein_bind 4093..4126 /label=loxP /note="Cre-mediated recombination occurs in the 8-bp core sequence (ATGTATGC) (Shaw et al., 2021)." rep_origin complement(4147..4596) /direction=LEFT /label=ARS1 /note="S. cerevisiae autonomously replicating sequence ARS1" misc_feature 4736..4876 /label=bom /note="basis of mobility region from pBR322" rep_origin complement(5062..5650) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(5824..6681) /codon_start=1 /label=AmpR /note="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(6682..6786) /label=AmpR promoter promoter 7042..7706 /label=GAL1,10 promoter /note="divergent inducible promoter, regulated by Gal4" ORIGIN 1 gaattcctcg aggaattcat aattttttcc tccagatcct ctagagtcct gtttcctgtg 61 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa 121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct 181 ttccagtcgg gaaacctgtc gtgccaggac ctgcaggcat gcaagctgcg gccggccgca 241 gctttgcaga ggctagcaga attaccctcc acgttgattg tctgcgaggc aagaatgatc 301 atcaccgtag tgagagtgcg ttcaaggctc ttgcggttgc cataagagaa gccacctcgc 361 ccaatggtac caacgatgtt ccctccacca aaggtgttct tatgtagtga caccgattat 421 ttaaagctgc agcatacgat atatatacat gtgtatatat gtatacctat gaatgtcagt 481 aagtatgtat acgaacagta tgatactgaa gatgacaagg taatgcatca ttctatacgt 541 gtcattctga acgaggcgcg ctttcctttt ttctttttgc tttttctttt tttttctctt 601 gaactcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg 661 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg 721 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga 781 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg 841 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg 901 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga 961 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg 1021 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt 1081 cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg 1141 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg 1201 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt 1261 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa 1321 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatccc 1381 cccccccctt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc 1441 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca 1501 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat 1561 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag 1621 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata 1681 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat 1741 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata 1801 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt 1861 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc 1921 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg 1981 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac 2041 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat 2101 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg 2161 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg 2221 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg 2281 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg 2341 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg 2401 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta 2461 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata 2521 atgacgaaaa aaaaaaaatt ggaaagaaaa gggggggggg gcagcgttgg gtcctggcca 2581 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt 2641 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa 2701 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg 2761 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc 2821 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg 2881 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt 2941 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt 3001 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa 3061 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa 3121 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct 3181 gatgagcttt accgcagctg ggccattctc atgaagaata tcttgaattt attgtcatat 3241 tactagttgg tgtggaagtc catatatcgg tgatcaatat agtggttgac atgctggcta 3301 gtcaacattg agccttttga tcatgcaaat atattacggt attttacaat caaatatcaa 3361 acttaactat tgactttata acttatttag gtggtaacat tcttataaaa aagaaaaaaa 3421 ttactgcaaa acagtactag cttttaactt gtatcctagg ttatctatgc tgtctcacca 3481 tagagaatat tacctatttc agaatgtatg tccatgattc gccgggtaaa tacatataat 3541 acacaaatct ggcttaataa agtctataat atatctcata aagaagtgct aaattggcta 3601 gtgctatata tttttaagaa aatttctttt gactaagtcc atatcgactt tgtaaaagtt 3661 cactttagca tacatatatt acacgagcca gaaattgtaa cttttgccta aaatcacaaa 3721 ttgcaaaatt taattgcttg caaaaggtca catgcttata atcaactttt ttaaaaattt 3781 aaaatacttt tttatttttt atttttaaac ataaatgaaa taatttattt attgtttatg 3841 attaccgaaa cataaaacct gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc 3901 actacctagg agcggccaaa atgccgaggc tttcatagct taaactcttt acagaaaata 3961 ggcattatag atcagttcga gttttcttat tcttccttcc ggttttatcg tcacagtttt 4021 acagtaaata agtatcacct cttagagttg cggccggtcg accaattccg atcatattca 4081 ataaccctta atataacttc gtataatgta tgctatacga agttattagg tccctcgacc 4141 ggccgcggcg gttgtttgca agaccgagaa aaggctagca agaatcgggt cattgtagcg 4201 tatgcgcctg tgaacattct cttcaacaag tttgattcca ttgcggtgaa atggtaaaag 4261 tcaaccccct gcgatgtata ttttcctgta caatcaatca aaaagccaaa tgatttagca 4321 ttatctttac atcttgttat tttacagatt ttatgtttag atcttttatg cttgcttttc 4381 aaaaggcctg caggcaagtg cacaaacaat acttaaataa atactactca gtaataacct 4441 atttcttagc atttttgacg aaatttgcta ttttgttaga gtcttttaca ccatttgtct 4501 ccacacctcc gcttacatca acaccaataa cgccatttaa tctaagcgca tcaccaacat 4561 tttctggcgt cagtccacca gctaacataa aatgttgcct cgcgcgtttc ggtgatgacg 4621 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg 4681 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag 4741 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga 4801 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 4861 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 4921 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 4981 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 5041 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 5101 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 5161 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 5221 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 5281 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 5341 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 5401 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 5461 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 5521 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 5581 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 5641 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 5701 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 5761 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 5821 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 5881 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 5941 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 6001 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 6061 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 6121 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 6181 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 6241 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 6301 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 6361 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 6421 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 6481 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 6541 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 6601 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 6661 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 6721 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 6781 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 6841 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat taattcgaca 6901 ggttatcagc aacaacacag tcatatccat tctcaattag ctctaccaca gtgtgtgaac 6961 caatgtatcc agcaccacct gtaaccaaaa caattttaga agtactttca ctttgtaact 7021 gagctgtcat ttatattgaa ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag 7081 aagtttaata atcatattac atggcattac caccatatac atatccatat acatatccat 7141 atctaatctt acttatatgt tgtggaaatg taaagagccc cattatctta gcctaaaaaa 7201 accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat tgaagtacgg 7261 attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc gtgcgtcctc 7321 gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa 7381 taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg 7441 ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata atgcgattag 7501 ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac 7561 agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt 7621 tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct 7681 atactttaac gtcaaggaga aaaaactata atgactaaat ctcattcaga agaagtgatt 7741 gtacctgagt tcaattctag cgcaaaggaa ttaccaagac cattggccga aaagtgcg //