LOCUS       40924_48713        7798 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector rpSE937, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7798)
  AUTHORS   Elledge SJ, Mulligan JT, Ramer SW, Spottswood M, Davis RW.
  TITLE     Lambda YES: a multifunctional cDNA expression vector for the 
            isolation of genes by complementation of yeast and Escherichia coli 
            mutations
  JOURNAL   Proc. Natl. Acad. Sci. U.S.A. 88 (5), 1731-1735 (1991)
  PUBMED    1848010
REFERENCE   2  (bases 1 to 7798)
  AUTHORS   Kitts PA.
  TITLE     CLONTECH Vectors On Disc version 1.3
  JOURNAL   Unpublished
REFERENCE   3  (bases 1 to 7798)
  AUTHORS   Kitts PA.
  TITLE     Direct Submission
  JOURNAL   Submitted (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 
            1020 East Meadow Circle, Palo Alto, CA 94303, USA
REFERENCE   4  (bases 1 to 7798)
  TITLE     Direct Submission
REFERENCE   5  (bases 1 to 7798)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc. Natl.
            Acad. Sci. U.S.A."; date: "1991"; volume: "88"; issue: "5"; pages: 
            "1731-1735"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 3; type: "Journal Article"; journalName: "Submitted 
            (07-OCT-1993) Paul A. Kitts, CLONTECH Laboratories, Inc., 1020 East 
            Meadow Circle, Palo Alto, CA 94303, USA"
COMMENT     SGRef: number: 4; type: "Journal Article"
COMMENT     This sequence has been compiled from information in the sequence 
            databases, published literature and other sources, together with 
            partial sequences obtained by CLONTECH. Lambda YES-P and pSE937 are 
            no longer available from CLONTECH and CLONTECH will not update or 
            revise this sequence.
FEATURES             Location/Qualifiers
     source          1..7798
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    complement(66..82)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(90..120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(135..156)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        1390..1610
                     /label=URA3 promoter
     CDS             1611..2411
                     /codon_start=1
                     /label=URA3
                     /note="orotidine-5'-phosphate decarboxylase, required for
                     uracil biosynthesis"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     misc_feature    complement(3757..4049)
                     /label=CEN4
                     /note="S. cerevisiae CEN4 centromere"
     protein_bind    4093..4126
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     rep_origin      complement(4147..4596)
                     /direction=LEFT
                     /label=ARS1
                     /note="S. cerevisiae autonomously replicating sequence
                     ARS1"
     misc_feature    4736..4876
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(5062..5650)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5824..6681)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6682..6786)
                     /label=AmpR promoter
     promoter        7042..7706
                     /label=GAL1,10 promoter
                     /note="divergent inducible promoter, regulated by Gal4"
ORIGIN
        1 gaattcctcg aggaattcat aattttttcc tccagatcct ctagagtcct gtttcctgtg
       61 tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct
      181 ttccagtcgg gaaacctgtc gtgccaggac ctgcaggcat gcaagctgcg gccggccgca
      241 gctttgcaga ggctagcaga attaccctcc acgttgattg tctgcgaggc aagaatgatc
      301 atcaccgtag tgagagtgcg ttcaaggctc ttgcggttgc cataagagaa gccacctcgc
      361 ccaatggtac caacgatgtt ccctccacca aaggtgttct tatgtagtga caccgattat
      421 ttaaagctgc agcatacgat atatatacat gtgtatatat gtatacctat gaatgtcagt
      481 aagtatgtat acgaacagta tgatactgaa gatgacaagg taatgcatca ttctatacgt
      541 gtcattctga acgaggcgcg ctttcctttt ttctttttgc tttttctttt tttttctctt
      601 gaactcgacc gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg
      661 gcatgactat cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg
      721 tgccggcagc gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga
      781 tcggcctgtc gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg
      841 gtcccgccac caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg
      901 cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga
      961 ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg
     1021 tagatgacga ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt
     1081 cgatcactgg accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg
     1141 ggttggcatg gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg
     1201 gtgcatggag ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt
     1261 caccactcca agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa
     1321 cccttggcag aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatccc
     1381 cccccccctt tcaattcaat tcatcatttt ttttttattc ttttttttga tttcggtttc
     1441 tttgaaattt ttttgattcg gtaatctccg aacagaagga agaacgaagg aaggagcaca
     1501 gacttagatt ggtatatata cgcatatgta gtgttgaaga aacatgaaat tgcccagtat
     1561 tcttaaccca actgcacaga acaaaaacct gcaggaaacg aagataaatc atgtcgaaag
     1621 ctacatataa ggaacgtgct gctactcatc ctagtcctgt tgctgccaag ctatttaata
     1681 tcatgcacga aaagcaaaca aacttgtgtg cttcattgga tgttcgtacc accaaggaat
     1741 tactggagtt agttgaagca ttaggtccca aaatttgttt actaaaaaca catgtggata
     1801 tcttgactga tttttccatg gagggcacag ttaagccgct aaaggcatta tccgccaagt
     1861 acaatttttt actcttcgaa gacagaaaat ttgctgacat tggtaataca gtcaaattgc
     1921 agtactctgc gggtgtatac agaatagcag aatgggcaga cattacgaat gcacacggtg
     1981 tggtgggccc aggtattgtt agcggtttga agcaggcggc agaagaagta acaaaggaac
     2041 ctagaggcct tttgatgtta gcagaattgt catgcaaggg ctccctatct actggagaat
     2101 atactaaggg tactgttgac attgcgaaga gcgacaaaga ttttgttatc ggctttattg
     2161 ctcaaagaga catgggtgga agagatgaag gttacgattg gttgattatg acacccggtg
     2221 tgggtttaga tgacaaggga gacgcattgg gtcaacagta tagaaccgtg gatgatgtgg
     2281 tctctacagg atctgacatt attattgttg gaagaggact atttgcaaag ggaagggatg
     2341 ctaaggtaga gggtgaacgt tacagaaaag caggctggga agcatatttg agaagatgcg
     2401 gccagcaaaa ctaaaaaact gtattataag taaatgcatg tatactaaac tcacaaatta
     2461 gagcttcaat ttaattatat cagttattac ccgggaatct cggtcgtaat gatttttata
     2521 atgacgaaaa aaaaaaaatt ggaaagaaaa gggggggggg gcagcgttgg gtcctggcca
     2581 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt
     2641 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa
     2701 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg
     2761 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc
     2821 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg
     2881 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt
     2941 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt
     3001 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa
     3061 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa
     3121 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct
     3181 gatgagcttt accgcagctg ggccattctc atgaagaata tcttgaattt attgtcatat
     3241 tactagttgg tgtggaagtc catatatcgg tgatcaatat agtggttgac atgctggcta
     3301 gtcaacattg agccttttga tcatgcaaat atattacggt attttacaat caaatatcaa
     3361 acttaactat tgactttata acttatttag gtggtaacat tcttataaaa aagaaaaaaa
     3421 ttactgcaaa acagtactag cttttaactt gtatcctagg ttatctatgc tgtctcacca
     3481 tagagaatat tacctatttc agaatgtatg tccatgattc gccgggtaaa tacatataat
     3541 acacaaatct ggcttaataa agtctataat atatctcata aagaagtgct aaattggcta
     3601 gtgctatata tttttaagaa aatttctttt gactaagtcc atatcgactt tgtaaaagtt
     3661 cactttagca tacatatatt acacgagcca gaaattgtaa cttttgccta aaatcacaaa
     3721 ttgcaaaatt taattgcttg caaaaggtca catgcttata atcaactttt ttaaaaattt
     3781 aaaatacttt tttatttttt atttttaaac ataaatgaaa taatttattt attgtttatg
     3841 attaccgaaa cataaaacct gctcaagaaa aagaaactgt tttgtccttg gaaaaaaagc
     3901 actacctagg agcggccaaa atgccgaggc tttcatagct taaactcttt acagaaaata
     3961 ggcattatag atcagttcga gttttcttat tcttccttcc ggttttatcg tcacagtttt
     4021 acagtaaata agtatcacct cttagagttg cggccggtcg accaattccg atcatattca
     4081 ataaccctta atataacttc gtataatgta tgctatacga agttattagg tccctcgacc
     4141 ggccgcggcg gttgtttgca agaccgagaa aaggctagca agaatcgggt cattgtagcg
     4201 tatgcgcctg tgaacattct cttcaacaag tttgattcca ttgcggtgaa atggtaaaag
     4261 tcaaccccct gcgatgtata ttttcctgta caatcaatca aaaagccaaa tgatttagca
     4321 ttatctttac atcttgttat tttacagatt ttatgtttag atcttttatg cttgcttttc
     4381 aaaaggcctg caggcaagtg cacaaacaat acttaaataa atactactca gtaataacct
     4441 atttcttagc atttttgacg aaatttgcta ttttgttaga gtcttttaca ccatttgtct
     4501 ccacacctcc gcttacatca acaccaataa cgccatttaa tctaagcgca tcaccaacat
     4561 tttctggcgt cagtccacca gctaacataa aatgttgcct cgcgcgtttc ggtgatgacg
     4621 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     4681 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     4741 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     4801 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     4861 aaaataccgc atcaggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt
     4921 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc
     4981 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa
     5041 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa
     5101 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc
     5161 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc
     5221 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag
     5281 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga
     5341 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc
     5401 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac
     5461 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg
     5521 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca
     5581 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     5641 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     5701 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     5761 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     5821 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     5881 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     5941 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     6001 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     6061 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     6121 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     6181 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     6241 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     6301 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     6361 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     6421 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct
     6481 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     6541 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     6601 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     6661 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     6721 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     6781 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac
     6841 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaat taattcgaca
     6901 ggttatcagc aacaacacag tcatatccat tctcaattag ctctaccaca gtgtgtgaac
     6961 caatgtatcc agcaccacct gtaaccaaaa caattttaga agtactttca ctttgtaact
     7021 gagctgtcat ttatattgaa ttttcaaaaa ttcttacttt ttttttggat ggacgcaaag
     7081 aagtttaata atcatattac atggcattac caccatatac atatccatat acatatccat
     7141 atctaatctt acttatatgt tgtggaaatg taaagagccc cattatctta gcctaaaaaa
     7201 accttctctt tggaactttc agtaatacgc ttaactgctc attgctatat tgaagtacgg
     7261 attagaagcc gccgagcggg tgacagccct ccgaaggaag actctcctcc gtgcgtcctc
     7321 gtcttcaccg gtcgcgttcc tgaaacgcag atgtgcctcg cgccgcactg ctccgaacaa
     7381 taaagattct acaatactag cttttatggt tatgaagagg aaaaattggc agtaacctgg
     7441 ccccacaaac cttcaaatga acgaatcaaa ttaacaacca taggatgata atgcgattag
     7501 ttttttagcc ttatttctgg ggtaattaat cagcgaagcg atgatttttg atctattaac
     7561 agatatataa atgcaaaaac tgcataacca ctttaactaa tactttcaac attttcggtt
     7621 tgtattactt cttattcaaa tgtaataaaa gtatcaacaa aaaattgtta atatacctct
     7681 atactttaac gtcaaggaga aaaaactata atgactaaat ctcattcaga agaagtgatt
     7741 gtacctgagt tcaattctag cgcaaaggaa ttaccaagac cattggccga aaagtgcg
//
