LOCUS       V014159                 9748 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V014159
VERSION     V014159
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 9748)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..9748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1087..1275
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1380..1520
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1706..2294)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(2468..3325)
                     /label="AmpR"
                     /note="beta-lactamase"
     terminator      4074..4108
                     /label="lambda t0 terminator"
                     /note="minimal transcription terminator from phage lambda
                     (Scholtissek and Grosse, 1987)"
     CDS             6445..7500
                     /gene="ERG20"
                     /label="Farnesyl pyrophosphate synthase"
                     /note="Farnesyl pyrophosphate synthase from Saccharomyces
                     cerevisiae (strain ATCC 204508 / S288c). Accession#:
                     P08524"
     CDS             7501..7518
                     /label="6xHis"
                     /note="6xHis affinity tag"
     promoter        complement(7567..7671)
                     /label="AmpR promoter"
     CDS             8050..8784
                     /gene="ermBP"
                     /label="rRNA adenine N-6-methyltransferase"
                     /note="rRNA adenine N-6-methyltransferase from Enterococcus
                     faecalis. Accession#: P0A4D5"
     terminator      8957..9043
                     /label="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      9062..9089
                     /label="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
ORIGIN
        1 atcctgatat tgtctgcatt tgcgccggag caaaccaaaa acctggtgag acacgccttg
       61 aattagtaga aaagaacttg aagattttca aaggcatcgt tagtgaagtc atggcgagcg
      121 gatttgacgg cattttctta gtcgcgacgc gaggctggat ggccttcccc attatgattc
      181 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag
      241 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga
      301 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt
      361 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg
      421 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac
      481 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc
      541 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg
      601 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct
      661 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg
      721 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt
      781 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat
      841 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg
      901 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt
      961 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat
     1021 cctctctcgt ttcatcggta tcattacccc catgaacaga aattccccct tacacggagg
     1081 catcaagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag
     1141 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt
     1201 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat
     1261 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg
     1321 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc
     1381 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat
     1441 cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac agatgcgtaa
     1501 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     1561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     1621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     1681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     1741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     1801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     1861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca atgctcacgc tgtaggtatc
     1921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     1981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     2101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     2161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     2221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     2281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     2341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     2401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     2461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     2521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     2581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     2641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     2701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     2761 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     2821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     2881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     2941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3061 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     3121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     3181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     3241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     3301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     3361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     3421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     3481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaatgatggg
     3541 ctaatgtgtg tttacgacaa ttctcacttc atacttttcc atcgtcaggt cgcctgacaa
     3601 tatgtctcct gtcattatgt ccttcacact ctgatcaaac gtgaccagct gtttttcttc
     3661 cgtgaaattc atgacaaaaa tataatcatt gtcctgatcc tgcctcgctt gtacggagac
     3721 gccttttccg tgccgaaccg gaaaaactgg agagagagac aggtctgtga tcagaccctc
     3781 atagaaatca cgctgaaatt gatcctccaa acgcgcgccg ataaaatacg ccttgccctg
     3841 ctgatactca tggcttgtga ccgctggcgt gcgcgcataa aaatcttctt gatacaccgc
     3901 ttccactgaa gctgtcttta catcaatcac ggttgcataa tccttcattt catatatttg
     3961 gctgcggtag ctgacagcgt ttcgatcctt cggatacagg gtgtccgttt caagaggctc
     4021 aactccaaat atagcttgaa atcgatatct ctgcagtcgc gatgattaat taattcagaa
     4081 cgctcggttg ccgccgggcg ttttttatgc agcaatggca agaacgtccc ggggagctcc
     4141 taacttatag gggtaacact taaaaaagaa tcaataacga tagaaaccgc tcctaaagca
     4201 ggtgcatttt ttcctaacga agaaggcaat agttcacatt tattgtctaa atgagaatgg
     4261 actctagaag aaacttcgtt tttaatcgta tttaaaacaa tgggatgaga ttcaattata
     4321 tgatttctca agataacagc ttctatatca aatgtattaa ggatattggt taatccaatt
     4381 ccgatataaa agccaaagtt ttgaagtgca tttaacattt ctacatcatt tttatttgcg
     4441 cgttccacaa tctcttttcg agaaatattc ttttcttctt tagagagcga agccagtaac
     4501 gctttttcag aagcatataa ttcccaacag cctcgatttc cacagctgca tttgggtcca
     4561 ttaaaatcta tcgtcatatg acccatttcc ccagaaaaac cctgaacacc tttatacaat
     4621 tcgttgttaa taacaagtcc agttccaatt ccgatattaa tactgatgta aacgatgttt
     4681 tcatagtttt ttgtcatacc aaatactttt tcaccgtatg ctcctgcatt agcttcattt
     4741 tcaacaaaaa ccggaacatt aaactcactc tcaattaaaa actgcaaatc tttgatattc
     4801 caatttaagt taggcatgaa aataatttgc tgatgacgat ctacaaggcc tggaacacaa
     4861 attcctattc cgactagacc ataaggggac tcaggcatat gggttacaaa accatgaata
     4921 agtgcaaata aaatctcttt tacttcacta gcggaagaac tagacaagtc agaagtcttc
     4981 tcgagaataa tatttccttc taagtcggtt agaattccgt taagatagtc gactcctata
     5041 tcaataccaa tcgagtagcc tgcattctta ttaaaaacaa gcattacagg tcttctgccg
     5101 cctctagatt gccctgcccc aatttcaaaa ataaaatctt tttcaagcag tgtatttact
     5161 tgagaggaga cagtagactt gtttaatcct gtaatctcag agagagttgc cctggagaca
     5221 ggggagttct tcaaaatttc atctaatatt aatttttgat tcattttttt tactaaagct
     5281 tgatctgcaa tttgaataat aaccactcct ttgtttatcc accgaactaa gttggtgttt
     5341 tttgaagctt gaattagata tttaaaagta tcatatctaa tattataact aaattttcta
     5401 aaaaaaacat tgaaataaac atttattttg tatatgatga gataaagtta gtttattgga
     5461 taaacaaact aactcaatta agatagttga tggataaact tgttcactta aatcaaaggg
     5521 ggaaatgaca aatggtccaa actagtgata tctaaaaatc aaagggggaa atgggatccg
     5581 gtaccattct gctagcagat gtctgttcct aacaaacttc gtgataacgc tatggttatg
     5641 cttaaagaaa catctcgtac attcttcatc cctatctctc atcttcctgc tgaacttcaa
     5701 aacgctgttg gctctgctta cctttgcatg cgtgctatcg atgaaatcga agatcatcct
     5761 gaacttgaag ctggcgttaa atctcgtctt ctttacgcta tctctgatct tcttaaaaaa
     5821 tctttcaacg aagatgaata catggctctt gttggccctt accaagctga tcttcctgat
     5881 gttacacttc aacttggcaa ctggatcgct ctttgcccta caggcgttgc tgatcaagtt
     5941 cttgatgcta catctatcat ggctaaaggc atggctgatt gggttgaaaa agattggcat
     6001 atccaaaacg aagctgatct tgatgattac acattctacg ttgctggcct tgttggcgtt
     6061 atgcttaacg atatctggaa atggtacgat ggcacagaaa cagataaaga acttgctatc
     6121 gctttcggcc gtggccttca atctgttaac atccttcgta acacatctga agattctgaa
     6181 cgtggcgttt ctttcttccc taacaactgg tctcgtgaag atatgttcgc ttacgctcgt
     6241 cgtaaccttt ctcttggcaa aaaatacctt gaagatgtta aatctcttcc tatccttcat
     6301 ttctgcaaaa tccctcttgc tcttgctgat ggcacactta acgctcttat gaaaggcaaa
     6361 gaaaaaatga cacgtgatga tgttaacaaa acagttaacg aagttgttga tatgcattaa
     6421 aggagaaata agaggaggag aaatatggca agcgaaaaag aaatcagaag agaaagattt
     6481 ctgaacgtgt ttccgaaact ggttgaagaa ttaaacgcga gcctgctggc gtatggcatg
     6541 ccgaaagaag catgcgattg gtatgcacat tcactgaatt ataatacacc gggcggcaaa
     6601 ctgaatcgcg gcctgtcagt ggttgatacg tatgcaattt tatctaataa aacggtggaa
     6661 caactgggac aagaagaata tgaaaaagtt gcaatcctgg gctggtgcat cgaactgctg
     6721 caggcatatt ttttagtggc ggatgatatg atggataaaa gcatcacaag aagaggacaa
     6781 ccttgctggt ataaagtgcc ggaagtggga gaaattgcaa ttaatgatgc gtttatgctt
     6841 gaagcggcga tttataaact gctgaaaagc cattttcgca acgaaaaata ttatatcgat
     6901 atcacggaac tgtttcatga agttacattt cagacagaac tgggccaatt gatggatctg
     6961 attacagcac cggaagataa agtggatctt tcaaaattta gcctgaaaaa gcattcattt
     7021 atcgtgacat ttaaaacggc atattattca ttttatctgc cggtagccct ggcaatgtat
     7081 gttgcaggca ttacagatga aaaagatctg aaacaagcaa gagatgtgct tatcccgctg
     7141 ggcgaatatt ttcaaattca agatgattat ctggattgct ttggaacacc ggaacagatt
     7201 ggaaaaatcg gcacagatat ccaagataac aaatgttcat gggttattaa caaagcactg
     7261 gaactggcat cagcagaaca aagaaaaaca ctggatgaaa attatggcaa aaaggatagc
     7321 gttgcagaag caaaatgcaa aaagattttt aacgatctga aaatcgaaca gctgtatcat
     7381 gaatatgaag aaagcatcgc aaaagatctg aaagcaaaaa tttcacaagt ggatgaatca
     7441 agaggattta aagcggatgt actgacagcc tttctgaaca aagtgtataa aagaagcaaa
     7501 catcatcatc atcatcatta actcccacgc gtcagctcct gtacaggatg agccgcgggc
     7561 ggccgcactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     7621 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     7681 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     7741 taggcgtatc acgaggccct ttcgtcttca agaattgatc ctctagcaca aaagaaaaac
     7801 gaaatgatac accaatcagt gcaaaaaaag atataatggg agataagacg gttcgtgttc
     7861 gtgctgactt gcaccatatc ataaaaatcg aaacagcaaa gaatggcgga aacgtaaaag
     7921 aagttatgga aataagactt agaagcaaac ttaagagtgt gttgatagtg cagtatctta
     7981 aaattttgta taataggaat tgaagttaaa ttagatgcta aaaatttgta attaagaagg
     8041 agtgattaca tgaacaaaaa tataaaatat tctcaaaact ttttaacgag tgaaaaagta
     8101 ctcaaccaaa taataaaaca attgaattta aaagaaaccg ataccgttta cgaaattgga
     8161 acaggtaaag ggcatttaac gacgaaactg gctaaaataa gtaaacaggt aacgtctatt
     8221 gaattagaca gtcatctatt caacttatcg tcagaaaaat taaaactgaa tactcgtgtc
     8281 actttaattc accaagatat tctacagttt caattcccta acaaacagag gtataaaatt
     8341 gttgggagta ttccttacca tttaagcaca caaattatta aaaaagtggt ttttgaaagc
     8401 catgcgtctg acatctatct gattgttgaa gaaggattct acaagcgtac cttggatatt
     8461 caccgaacac tagggttgct cttgcacact caagtctcga ttcagcaatt gcttaagctg
     8521 ccagcggaat gctttcatcc taaaccaaaa gtaaacagtg tcttaataaa acttacccgc
     8581 cataccacag atgttccaga taaatattgg aagctatata cgtactttgt ttcaaaatgg
     8641 gtcaatcgag aatatcgtca actgtttact aaaaatcagt ttcatcaagc aatgaaacac
     8701 gccaaagtaa acaatttaag taccgttact tatgagcaag tattgtctat ttttaatagt
     8761 tatctattat ttaacgggag gaaataattc tatgagtcgc ttttgtaaat ttggaaagtt
     8821 acacgttact aaagggaatg tagataaatt attaggtata ctactgacag cttccaagga
     8881 gctaaagagg tccctagact ctagacccgg ggatctctgc agtgagatct ggtaatgact
     8941 ctctagcttg aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt
     9001 tatctgttgt ttgtcggtga acgctctcct gagtaggaca aatccgccgc tctagctaag
     9061 cagaaggcca tcctgacgga tggccttttt gcgtttctac aaactcttgt taactctaga
     9121 gctgcctgcc gcgtttcggt gatgaagatc ttcccgatga ttaattaatt cagaacgctc
     9181 ggttgccgcc gggcgttttt tatgcagcaa tggcaagaac gttgctctag agcggccgca
     9241 tcgattcaca gtggcaatct cccccgtatt cgtttgaaat gtgccacatt aacagcgccg
     9301 ggtgatgtcc gtatcgttct gctaataagc ggttgatgtg ccgtgttttt tctcggtaga
     9361 ctttagatgt gaggcagtgg ttgtgccttc cgccgtgcag ctgtttgacg cgggaggcat
     9421 tgacgcgcaa aacttccgga taggtttgcg acagccaggc cggacgggct ccgctcggcg
     9481 ttgctaatat gacccggccg cctatactgt gaatccgctc aaaaatatca tccagccatt
     9541 caaattgata tacgccctcc tccggctcaa gtgcgctcca tgcaaaaatg ccgacagaaa
     9601 acgtattcgt atgagaaagc ttcatcagtt tgatatcgtc agctaaaata tcgggccgat
     9661 ccagccactg atcggggttg tagtctcccc catggagcat aaattttgct tttgttacgt
     9721 gcgttttttc aagctttgac atcacccc
//
