LOCUS       62056_20085        3748 bp DNA     circular SYN 03-DEC-2019
DEFINITION  Cloning vector pso_IA1_YQR_DIMER, complete sequence.
ACCESSION   MN207951
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3748)
  AUTHORS   Rondon RE, Groseclose TM, Short AE, Wilson CJ.
  TITLE     Transcriptional programming using engineered systems of 
            transcription factors and genetic architectures
  JOURNAL   Nat Commun 10 (1), 4784 (2019)
  PUBMED    31636266
REFERENCE   2  (bases 1 to 3748)
  AUTHORS   Rondon RE, Groseclose T, Short A, Wilson CJ.
  TITLE     Direct Submission
  JOURNAL   Submitted (21-JUL-2019) Chemical and Biomolecular Engineering, 
            Georgia Institute of Technology, 950 Atlantic dr. NW, 5110E, 
            Atlanta, GA 30332, United States
REFERENCE   3  (bases 1 to 3748)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun"; date: "2019"; volume: "10"; issue: "1"; pages: "4784"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (21-JUL-2019) Chemical and Biomolecular Engineering, Georgia 
            Institute of Technology, 950 Atlantic dr. NW, 5110E, Atlanta, GA 
            30332, United States"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..3748
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             101..289
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    394..536
                     /label=bom
                     /note="basis of mobility region from pBR322"
     promoter        693..797
                     /label=AmpR promoter
     CDS             798..1655
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      1829..2417
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        2553..2630
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             2631..3626
                     /codon_start=1
                     /transl_table=11
                     /product="IA1 YQR Dimer"
                     /label=IA1 YQR Dimer
                     /protein_id="QFU95578.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIASRADQLGASVVVSMVERSGV
                     EACKTAVHTLLAQRVSGLIINYPLDDQEAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTIQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGAVISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLA"
ORIGIN
        1 ggcatgttca tcatcagtaa cccgtatcgt gagcatcctc tctcgtttca tcggtatcat
       61 tacccccatg aacagaaatc ccccttacac ggaggcatca gtgaccaaac aggaaaaaac
      121 cgcccttaac atggcccgct ttatcagaag ccagacatta acgcttctgg agaaactcaa
      181 cgagctggac gcggatgaac aggcagacat ctgtgaatcg cttcacgacc acgctgatga
      241 gctttaccgc agctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca
      301 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca
      361 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga
      421 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac
      481 catatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
      541 cttccgcttc ctcgctcact gactcgctgc gctcaccgtc atcaccgaaa cgcgcgagac
      601 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
      661 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
      721 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
      781 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
      841 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
      901 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
      961 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     1021 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     1081 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     1141 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     1201 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     1261 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     1321 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     1381 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     1441 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     1501 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     1561 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     1621 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     1681 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     1741 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     1801 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     1861 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     1921 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     1981 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     2041 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     2101 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     2161 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     2221 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     2281 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     2341 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     2401 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     2461 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     2521 ccgcctttga gtgagctgat accgctcgcc gcgacaccat cgaatggtgc aaaacctttc
     2581 gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag
     2641 taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg
     2701 tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg
     2761 agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga
     2821 ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgattg
     2881 cttctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg
     2941 tcgaagcctg taaaacggcg gtgcacactc ttctcgcgca acgcgtcagt gggctgatca
     3001 ttaactatcc gctggatgac caggaagcca ttgctgtgga agctgcctgc actaatgttc
     3061 cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg
     3121 aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc
     3181 tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat
     3241 atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt
     3301 ccggttttca acaaaccata caaatgctga atgagggcat cgttcccact gcgatgctgg
     3361 ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg
     3421 ttggtgcggt tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc
     3481 cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct
     3541 tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg
     3601 tgaaaagaaa aaccaccctg gcgtaataaa cgcaaaccgc ctctccccgc gcgttggccg
     3661 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgactcagga
     3721 ttctggcgta ccgttcctgt ctaaaatc
//
