LOCUS       62056_19725        6285 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6285)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6285
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             709..1638
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="ASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAENA
                     VIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAWF
                     ELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDIA
                     LIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPREI
                     PLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKVK
                     GLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1700..1748
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        1757..2508
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             2544..4193
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(4240..4361)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        4494..4598
                     /label=AmpR promoter
     CDS             4599..5456
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5630..6218
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatgaaaa agaagaaagc ttccaaggtg
      721 tacgaccccg agcaacgcaa acgcatgatc actgggcctc agtggtgggc tcgctgcaag
      781 caaatgaacg tgctggactc cttcatcaac tactatgatt ccgagaagca cgccgagaac
      841 gccgtgattt ttctgcatgg taacgctgcc tccagctacc tgtggaggca cgtcgtgcct
      901 cacatcgagc ccgtggctag atgcatcatc cctgatctga tcggaatggg taagtccggc
      961 aagagcggga atggctcata tcgcctcctg gatcactaca agtacctcac cgcttggttc
     1021 gagctgctga accttccaaa gaaaatcatc tttgtgggcc acgactgggg ggcttgtctg
     1081 gcctttcact actcctacga gcaccaagac aagatcaagg ccatcgtcca tgctgagagt
     1141 gtcgtggacg tgatcgagtc ctgggacgag tggcctgaca tcgaggagga tatcgccctg
     1201 atcaagagcg aagagggcga gaaaatggtg cttgagaata acttcttcgt cgagaccatg
     1261 ctcccaagca agatcatgcg gaaactggag cctgaggagt tcgctgccta cctggagcca
     1321 ttcaaggaga agggcgaggt tagacggcct accctctcct ggcctcgcga gatccctctc
     1381 gttaagggag gcaagcccga cgtcgtccag attgtccgca actacaacgc ctaccttcgg
     1441 gccagcgacg atctgcctaa gatgttcatc gagtccgacc ctgggttctt ttccaacgct
     1501 attgtcgagg gagctaagaa gttccctaac accgagttcg tgaaggtgaa gggcctccac
     1561 ttcagccagg aggacgctcc agatgaaatg ggtaagtaca tcaagagctt cgtggagcgc
     1621 gtgctgaaga acgagcagta attctaggcg atcgctcgag cccgggaatt cgtttaaacc
     1681 tagagcggcc gctggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt
     1741 tttgtgtgag gatctaaatg agtcttcgga cctcgcgggg gccgcttaag cggtggttag
     1801 ggtttgtctg acgcgggggg agggggaagg aacgaaacac tctcattcgg aggcggctcg
     1861 gggtttggtc ttggtggcca cgggcacgca gaagagcgcc gcgatcctct taagcacccc
     1921 cccgccctcc gtggaggcgg gggtttggtc ggcgggtggt aactggcggg ccgctgactc
     1981 gggcgggtcg cgcgccccag agtgtgacct tttcggtctg ctcgcagacc cccgggcggc
     2041 gccgccgcgg cggcgacggg ctcgctgggt cctaggctcc atggggaccg tatacgtgga
     2101 caggctctgg agcatccgca cgactgcggt gatattaccg gagaccttct gcgggacgag
     2161 ccgggtcacg cggctgacgc ggagcgtccg ttgggcgaca aacaccagga cggggcacag
     2221 gtacactatc ttgtcacccg gaggcgcgag ggactgcagg agcttcaggg agtggcgcag
     2281 ctgcttcatc cccgtggccc gttgctcgcg tttgctggcg gtgtccccgg aagaaatata
     2341 tttgcatgtc tttagttcta tgatgacaca aaccccgccc agcgtcttgt cattggcgaa
     2401 ttcgaacacg cagatgcagt cggggcggcg cggtcccagg tccacttcgc atattaaggt
     2461 gacgcgtgtg gcctcgaaca ccgagcgacc ctgcagcgac ccgcttaaaa gcttggcatt
     2521 ccggtactgt tggtaaagcc accatggccg atgctaagaa cattaagaag ggccctgctc
     2581 ccttctaccc tctggaggat ggcaccgctg gcgagcagct gcacaaggcc atgaagaggt
     2641 atgccctggt gcctggcacc attgccttca ccgatgccca cattgaggtg gacatcacct
     2701 atgccgagta cttcgagatg tctgtgcgcc tggccgaggc catgaagagg tacggcctga
     2761 acaccaacca ccgcatcgtg gtgtgctctg agaactctct gcagttcttc atgccagtgc
     2821 tgggcgccct gttcatcgga gtggccgtgg cccctgctaa cgacatttac aacgagcgcg
     2881 agctgctgaa cagcatgggc atttctcagc ctaccgtggt gttcgtgtct aagaagggcc
     2941 tgcagaagat cctgaacgtg cagaagaagc tgcctatcat ccagaagatc atcatcatgg
     3001 actctaagac cgactaccag ggcttccaga gcatgtacac attcgtgaca tctcatctgc
     3061 ctcctggctt caacgagtac gacttcgtgc cagagtcttt cgacagggac aaaaccattg
     3121 ccctgatcat gaacagctct gggtctaccg gcctgcctaa gggcgtggcc ctgcctcatc
     3181 gcaccgcctg tgtgcgcttc tctcacgccc gcgaccctat tttcggcaac cagatcatcc
     3241 ccgacaccgc tattctgagc gtggtgccat tccaccacgg cttcggcatg ttcaccaccc
     3301 tgggctacct gatttgcggc tttcgggtgg tgctgatgta ccgcttcgag gaggagctgt
     3361 tcctgcgcag cctgcaagac tacaaaattc agtctgccct gctggtgcca accctgttca
     3421 gcttcttcgc taagagcacc ctgatcgaca agtacgacct gtctaacctg cacgagattg
     3481 cctctggcgg cgccccactg tctaaggagg tgggcgaagc cgtggccaag cgctttcatc
     3541 tgccaggcat ccgccagggc tacggcctga ccgagacaac cagcgccatt ctgattaccc
     3601 cagagggcga cgacaagcct ggcgccgtgg gcaaggtggt gccattcttc gaggccaagg
     3661 tggtggacct ggacaccggc aagaccctgg gagtgaacca gcgcggcgag ctgtgtgtgc
     3721 gcggccctat gattatgtcc ggctacgtga ataaccctga ggccacaaac gccctgatcg
     3781 acaaggacgg ctggctgcac tctggcgaca ttgcctactg ggacgaggac gagcacttct
     3841 tcatcgtgga ccgcctgaag tctctgatca agtacaaggg ctaccaggtg gccccagccg
     3901 agctggagtc tatcctgctg cagcacccta acattttcga cgccggagtg gccggcctgc
     3961 ccgacgacga tgccggcgag ctgcctgccg ccgtcgtcgt gctggaacac ggcaagacca
     4021 tgaccgagaa ggagatcgtg gactatgtgg ccagccaggt gacaaccgcc aagaagctgc
     4081 gcggcggagt ggtgttcgtg gacgaggtgc ccaagggcct gaccggcaag ctggacgccc
     4141 gcaagatccg cgagatcctg atcaaggcta agaaaggcgg caagatcgcc gtgtaataat
     4201 tctagagtcg gggcggccgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     4261 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4321 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4381 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaaagcaag taaaacctct
     4441 acaaatgtgg taaaatcgat aaggatccag gtggcacttt tcggggaaat gtgcgcggaa
     4501 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     4561 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     4621 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     4681 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     4741 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     4801 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     4861 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     4921 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     4981 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5041 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5101 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5161 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5221 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5281 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     5341 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     5401 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     5461 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     5521 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     5581 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     5641 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     5701 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     5761 agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     5821 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     5881 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     5941 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     6001 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     6061 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     6121 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     6181 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     6241 tacggttcct ggccttttgc tggccttttg ctcacatggc tcgac
//
