LOCUS       62056_19740        7577 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7577)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..7577
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..1626
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    2992..3040
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        3049..3800
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3836..5485
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(5532..5653)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5786..5890
                     /label=AmpR promoter
     CDS             5891..6748
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6922..7510
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagac ctatcccggg acagggcctc ttcctcggcc
     1681 ttcccatatt ggtggcagtg gtgccacact gaacagagtg gaagacatat gccatgcagc
     1741 tacacctacc ggccctggga cgccggagga cagggcattg tcctcagtca gatacaacag
     1801 catttggggc catggtacct gcacacctaa aacactaggc cacgcatctg atctgtagtc
     1861 acatgactaa gccaagagga aggagcaaga ctcaagacat gattgatgga tgttaaagtc
     1921 tagcctgatg agaggggaag tggtggggga gacatagccc caccatgagg acatacaact
     1981 gggaaatact gaaacttgct gcctattggg tatgctgagg ccccacagac ttacagaaga
     2041 agtggccctc catagacatg tgtagcatca aaacacaaag gcccacactt cctgacggat
     2101 gccagcttgg gcactgctgt ctactgaccc caacccttga tgatatgtat ttattcattt
     2161 gttattttac cagctattta ttgagtgtct tttatgtagg ctaaatgaac ataggtctct
     2221 ggcctcacgg agctcccagt cctaatcaca ttcaaggtca ccaggtacag ttgtacaggt
     2281 tgtacactgc aggagagtgc ctggcaaaaa gatcaaatgg ggctgggact tctcattggc
     2341 caacctgcct ttccccagaa ggagtgattt ttctatcggc acaaaagcac tatatggact
     2401 ggtaatggtt acaggttcag agattaccca gtgaggcctt attcctccct tccccccaaa
     2461 actgacacct ttgttagcca cctccccacc cacatacatt tctgccagtg ttcacaatga
     2521 cactcagcgg tcatgtctgg acatgagtgc ccagggaata tgcccaagct atgccttgtc
     2581 ctcttgtcct gtttgcattt cactgggagc ttgcactatg cagctccagt ttcctgcagt
     2641 gatcagggtc ctgcaagcag tggggaaggg ggccaaggta ttggaggact ccctcccagc
     2701 tttggaagcc tcatccgcgt gtgtgtgtgt gtgtatgtgt agacaagctc tcgctctgtc
     2761 acccaggctg gagtgcagtg gtgcaatcat ggttcactgc agtcttgacc ttttgggctc
     2821 aagtgatcct cccacctcag cctcctgagt agctgggacc ataggctcac aacaccacac
     2881 ctggcaaatt tgattttttt tttttttcca gagacggggt ctcgcaacat tgcccagact
     2941 tcctttgtgt tagttaataa agctttctca actgccgcgg ccgctggccg caataaaata
     3001 tctttatttt cattacatct gtgtgttggt tttttgtgtg aggatctaaa tgagtcttcg
     3061 gacctcgcgg gggccgctta agcggtggtt agggtttgtc tgacgcgggg ggagggggaa
     3121 ggaacgaaac actctcattc ggaggcggct cggggtttgg tcttggtggc cacgggcacg
     3181 cagaagagcg ccgcgatcct cttaagcacc cccccgccct ccgtggaggc gggggtttgg
     3241 tcggcgggtg gtaactggcg ggccgctgac tcgggcgggt cgcgcgcccc agagtgtgac
     3301 cttttcggtc tgctcgcaga cccccgggcg gcgccgccgc ggcggcgacg ggctcgctgg
     3361 gtcctaggct ccatggggac cgtatacgtg gacaggctct ggagcatccg cacgactgcg
     3421 gtgatattac cggagacctt ctgcgggacg agccgggtca cgcggctgac gcggagcgtc
     3481 cgttgggcga caaacaccag gacggggcac aggtacacta tcttgtcacc cggaggcgcg
     3541 agggactgca ggagcttcag ggagtggcgc agctgcttca tccccgtggc ccgttgctcg
     3601 cgtttgctgg cggtgtcccc ggaagaaata tatttgcatg tctttagttc tatgatgaca
     3661 caaaccccgc ccagcgtctt gtcattggcg aattcgaaca cgcagatgca gtcggggcgg
     3721 cgcggtccca ggtccacttc gcatattaag gtgacgcgtg tggcctcgaa caccgagcga
     3781 ccctgcagcg acccgcttaa aagcttggca ttccggtact gttggtaaag ccaccatggc
     3841 cgatgctaag aacattaaga agggccctgc tcccttctac cctctggagg atggcaccgc
     3901 tggcgagcag ctgcacaagg ccatgaagag gtatgccctg gtgcctggca ccattgcctt
     3961 caccgatgcc cacattgagg tggacatcac ctatgccgag tacttcgaga tgtctgtgcg
     4021 cctggccgag gccatgaaga ggtacggcct gaacaccaac caccgcatcg tggtgtgctc
     4081 tgagaactct ctgcagttct tcatgccagt gctgggcgcc ctgttcatcg gagtggccgt
     4141 ggcccctgct aacgacattt acaacgagcg cgagctgctg aacagcatgg gcatttctca
     4201 gcctaccgtg gtgttcgtgt ctaagaaggg cctgcagaag atcctgaacg tgcagaagaa
     4261 gctgcctatc atccagaaga tcatcatcat ggactctaag accgactacc agggcttcca
     4321 gagcatgtac acattcgtga catctcatct gcctcctggc ttcaacgagt acgacttcgt
     4381 gccagagtct ttcgacaggg acaaaaccat tgccctgatc atgaacagct ctgggtctac
     4441 cggcctgcct aagggcgtgg ccctgcctca tcgcaccgcc tgtgtgcgct tctctcacgc
     4501 ccgcgaccct attttcggca accagatcat ccccgacacc gctattctga gcgtggtgcc
     4561 attccaccac ggcttcggca tgttcaccac cctgggctac ctgatttgcg gctttcgggt
     4621 ggtgctgatg taccgcttcg aggaggagct gttcctgcgc agcctgcaag actacaaaat
     4681 tcagtctgcc ctgctggtgc caaccctgtt cagcttcttc gctaagagca ccctgatcga
     4741 caagtacgac ctgtctaacc tgcacgagat tgcctctggc ggcgccccac tgtctaagga
     4801 ggtgggcgaa gccgtggcca agcgctttca tctgccaggc atccgccagg gctacggcct
     4861 gaccgagaca accagcgcca ttctgattac cccagagggc gacgacaagc ctggcgccgt
     4921 gggcaaggtg gtgccattct tcgaggccaa ggtggtggac ctggacaccg gcaagaccct
     4981 gggagtgaac cagcgcggcg agctgtgtgt gcgcggccct atgattatgt ccggctacgt
     5041 gaataaccct gaggccacaa acgccctgat cgacaaggac ggctggctgc actctggcga
     5101 cattgcctac tgggacgagg acgagcactt cttcatcgtg gaccgcctga agtctctgat
     5161 caagtacaag ggctaccagg tggccccagc cgagctggag tctatcctgc tgcagcaccc
     5221 taacattttc gacgccggag tggccggcct gcccgacgac gatgccggcg agctgcctgc
     5281 cgccgtcgtc gtgctggaac acggcaagac catgaccgag aaggagatcg tggactatgt
     5341 ggccagccag gtgacaaccg ccaagaagct gcgcggcgga gtggtgttcg tggacgaggt
     5401 gcccaagggc ctgaccggca agctggacgc ccgcaagatc cgcgagatcc tgatcaaggc
     5461 taagaaaggc ggcaagatcg ccgtgtaata attctagagt cggggcggcc ggccgcttcg
     5521 agcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     5581 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     5641 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
     5701 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaaaatcg ataaggatcc
     5761 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5821 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5881 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5941 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     6001 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     6061 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     6121 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     6181 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     6241 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     6301 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     6361 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     6421 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     6481 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     6541 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     6601 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     6661 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6721 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6781 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6841 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6901 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6961 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     7021 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     7081 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     7141 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     7201 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     7261 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     7321 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     7381 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     7441 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     7501 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     7561 tgctcacatg gctcgac
//
