LOCUS       62056_19710        6849 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6849)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6849
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        62..419
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          489..621
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        666..684
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             694..1626
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    2264..2312
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     promoter        2321..3072
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             3108..4757
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MADAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     polyA_signal    complement(4804..4925)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        5058..5162
                     /label=AmpR promoter
     CDS             5163..6020
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6194..6782
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg ttaggtacct
       61 tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
      121 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
      181 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      241 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
      301 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
      361 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
      421 agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg tcgtgaggca
      481 ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct
      541 tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc
      601 actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct taaggctaga
      661 gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta cgaccccgag
      721 caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca aatgaacgtg
      781 ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc cgtgattttt
      841 ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca catcgagccc
      901 gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa gagcgggaat
      961 ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga gctgctgaac
     1021 cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc ctttcactac
     1081 tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt cgtggacgtg
     1141 atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat caagagcgaa
     1201 gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct cccaagcaag
     1261 atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt caaggagaag
     1321 ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt taagggaggc
     1381 aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc cagcgacgat
     1441 ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat tgtcgaggga
     1501 gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt cagccaggag
     1561 gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt gctgaagaac
     1621 gagcagtaat tctaggcgat cgctcgagcc cacggtactt actccccact gatggaccag
     1681 tggtttccag tcatgagcgt tagactgact tgtttgtctt ccattccatt gttttgaaac
     1741 tcagtatgct gcccctgtct tgctgtcatg aaatcagcaa gagaggatga cacatcaaat
     1801 aataactcgg attccagccc acattggatt catcagcatt tggaccaata gcccacagct
     1861 gagaatgtgg aatacctaag gatagcaccg cttttgttct cgcaaaaacg tatctcctaa
     1921 tttgaggctc agatgaaatg catcaggtcc tttggggcat agatcagaag actacaaaaa
     1981 tgaagctgct ctgaaatctc ctttagccat caccccaacc ccccaaaatt agtttgtgtt
     2041 acttatggaa gatagttttc tccttttact tcacttcaaa agctttttac tcaaagagta
     2101 tatgttccct ccaggtcagc tgcccccaaa ccccctcctt acgctttgtc acacaaaaag
     2161 tgtctctgcc ttgagtcatc tattcaagca cttacagctc tggccacaac agggcatttt
     2221 acaggtgcga atgacagtag cattatgagc ggccgctggc cgcaataaaa tatctttatt
     2281 ttcattacat ctgtgtgttg gttttttgtg tgaggatcta aatgagtctt cggacctcgc
     2341 gggggccgct taagcggtgg ttagggtttg tctgacgcgg ggggaggggg aaggaacgaa
     2401 acactctcat tcggaggcgg ctcggggttt ggtcttggtg gccacgggca cgcagaagag
     2461 cgccgcgatc ctcttaagca cccccccgcc ctccgtggag gcgggggttt ggtcggcggg
     2521 tggtaactgg cgggccgctg actcgggcgg gtcgcgcgcc ccagagtgtg accttttcgg
     2581 tctgctcgca gacccccggg cggcgccgcc gcggcggcga cgggctcgct gggtcctagg
     2641 ctccatgggg accgtatacg tggacaggct ctggagcatc cgcacgactg cggtgatatt
     2701 accggagacc ttctgcggga cgagccgggt cacgcggctg acgcggagcg tccgttgggc
     2761 gacaaacacc aggacggggc acaggtacac tatcttgtca cccggaggcg cgagggactg
     2821 caggagcttc agggagtggc gcagctgctt catccccgtg gcccgttgct cgcgtttgct
     2881 ggcggtgtcc ccggaagaaa tatatttgca tgtctttagt tctatgatga cacaaacccc
     2941 gcccagcgtc ttgtcattgg cgaattcgaa cacgcagatg cagtcggggc ggcgcggtcc
     3001 caggtccact tcgcatatta aggtgacgcg tgtggcctcg aacaccgagc gaccctgcag
     3061 cgacccgctt aaaagcttgg cattccggta ctgttggtaa agccaccatg gccgatgcta
     3121 agaacattaa gaagggccct gctcccttct accctctgga ggatggcacc gctggcgagc
     3181 agctgcacaa ggccatgaag aggtatgccc tggtgcctgg caccattgcc ttcaccgatg
     3241 cccacattga ggtggacatc acctatgccg agtacttcga gatgtctgtg cgcctggccg
     3301 aggccatgaa gaggtacggc ctgaacacca accaccgcat cgtggtgtgc tctgagaact
     3361 ctctgcagtt cttcatgcca gtgctgggcg ccctgttcat cggagtggcc gtggcccctg
     3421 ctaacgacat ttacaacgag cgcgagctgc tgaacagcat gggcatttct cagcctaccg
     3481 tggtgttcgt gtctaagaag ggcctgcaga agatcctgaa cgtgcagaag aagctgccta
     3541 tcatccagaa gatcatcatc atggactcta agaccgacta ccagggcttc cagagcatgt
     3601 acacattcgt gacatctcat ctgcctcctg gcttcaacga gtacgacttc gtgccagagt
     3661 ctttcgacag ggacaaaacc attgccctga tcatgaacag ctctgggtct accggcctgc
     3721 ctaagggcgt ggccctgcct catcgcaccg cctgtgtgcg cttctctcac gcccgcgacc
     3781 ctattttcgg caaccagatc atccccgaca ccgctattct gagcgtggtg ccattccacc
     3841 acggcttcgg catgttcacc accctgggct acctgatttg cggctttcgg gtggtgctga
     3901 tgtaccgctt cgaggaggag ctgttcctgc gcagcctgca agactacaaa attcagtctg
     3961 ccctgctggt gccaaccctg ttcagcttct tcgctaagag caccctgatc gacaagtacg
     4021 acctgtctaa cctgcacgag attgcctctg gcggcgcccc actgtctaag gaggtgggcg
     4081 aagccgtggc caagcgcttt catctgccag gcatccgcca gggctacggc ctgaccgaga
     4141 caaccagcgc cattctgatt accccagagg gcgacgacaa gcctggcgcc gtgggcaagg
     4201 tggtgccatt cttcgaggcc aaggtggtgg acctggacac cggcaagacc ctgggagtga
     4261 accagcgcgg cgagctgtgt gtgcgcggcc ctatgattat gtccggctac gtgaataacc
     4321 ctgaggccac aaacgccctg atcgacaagg acggctggct gcactctggc gacattgcct
     4381 actgggacga ggacgagcac ttcttcatcg tggaccgcct gaagtctctg atcaagtaca
     4441 agggctacca ggtggcccca gccgagctgg agtctatcct gctgcagcac cctaacattt
     4501 tcgacgccgg agtggccggc ctgcccgacg acgatgccgg cgagctgcct gccgccgtcg
     4561 tcgtgctgga acacggcaag accatgaccg agaaggagat cgtggactat gtggccagcc
     4621 aggtgacaac cgccaagaag ctgcgcggcg gagtggtgtt cgtggacgag gtgcccaagg
     4681 gcctgaccgg caagctggac gcccgcaaga tccgcgagat cctgatcaag gctaagaaag
     4741 gcggcaagat cgccgtgtaa taattctaga gtcggggcgg ccggccgctt cgagcagaca
     4801 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     4861 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     4921 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     4981 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccaggtggca
     5041 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     5101 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     5161 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     5221 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     5281 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     5341 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     5401 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     5461 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     5521 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     5581 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     5641 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     5701 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     5761 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     5821 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     5881 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     5941 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     6001 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     6061 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     6121 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     6181 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     6241 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     6301 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     6361 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     6421 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     6481 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     6541 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     6601 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     6661 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     6721 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     6781 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     6841 tggctcgac
//
