LOCUS       .                      11108 bp    DNA     circular UNK 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   
VERSION     
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
FEATURES             Location/Qualifiers
     enhancer        73..376
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     intron          654..1670
                     /label="chimeric intron"
                     /note="chimera between introns from chicken β-actin and
                     rabbit β-globin"
     CDS             3470..6478
                     /label="HIV-1 pol"
                     /note="pol protein from human immunodeficiency virus 1"
     CDS             6575..6748
                     /gene="Tat"
                     /label="Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate BH5). Accession#: P04612"
     misc_feature    7055..7288
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             7473..7517
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             7666..7707
                     /gene="Protein Tat"
                     /label="Protein Tat"
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
     CDS             7811..7837
                     /label="NES"
                     /note="nuclear export signal from the HIV Rev protein
                     (Fischer et al., 1995)"
     polyA_signal    8187..8242
                     /label="β-globin poly(A) signal"
                     /note="rabbit β-globin polyadenylation signal (Gil and
                     Proudfoot, 1987)"
     promoter        complement(8651..8681)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(8696..8717)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8775..8971
                     /label="SV40 promoter"
                     /note="SV40 early promoter"
     polyA_signal    8977..9111
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(9350..9938)
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(10112..10969)
                     /label="AmpR"
                     /note="β-lactamase"
ORIGIN
        1 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
       61 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      121 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
      181 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
      241 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
      301 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
      361 catcgctatt accatggtcg aggtgagccc cacgttctgc ttcactctcc ccatctcccc
      421 cccctcccca cccccaattt tgtatttatt tattttttaa ttattttgtg cagcgatggg
      481 ggcggggggg gggggggggc gcgccrgggg gggggggggg gggggggggg gggggggggg
      541 sgrggsggrg rggkgcggcg gcagccaatc agagcggcgc gctccgaaag tttcctttta
      601 tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg
      661 ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc ccgccccggc
      721 tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct cctccgggct
      781 gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt gaaagccttg
      841 aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggggtgcg tgcgtgtgtg
      901 tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg cggctgtgag cgctgcgggc
      961 gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg gagcgcggcc gggggcggtg
     1021 ccccgcggtg cggggggggc tgcgagggga acaaaggctg cgtgcggggt gtgtgcgtgg
     1081 gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca accccccctg cacccccctc
     1141 cccgagttgc tgagcacggc ccggcttcgg gtgcggggct ccgtacgggg cgtggcgcgg
     1201 ggctcgccgt gccgggcggg gggtggcggc aggtgggggt gccgggcggg gcggggccgc
     1261 cycgggccgg ggrgggctcg ggggrggggc gcggcggccc ccggagcgcc ggcggctgtc
     1321 gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg gcgcagggac
     1381 ttcctttgtc ccaaatctgt gcggagccga aatctgggag gcgccgccgc accccctcta
     1441 gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc
     1501 gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc tcggggctgt ccgcgggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgggccg cgttgacgcg
     1741 cacggcaaga ggcgaggggc ggcgactggt gagagatggg tgcgagagcg tcagtattaa
     1801 gcgggggaga attagatcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaaaaaagca cagcaagcag cagctgacac aggacacagc aatcaggtca
     2161 gccaaaatta ccctatagtg cagaacatcc aggggcaaat ggtacatcag gccatatcac
     2221 ctagaacttt aaatgcatgg gtaaaagtag tagaagagaa ggctttcagc ccagaagtga
     2281 tacccatgtt ttcagcatta tcagaaggag ccaccccaca agatttaaac accatgctaa
     2341 acacagtggg gggacatcaa gcagccatgc aaatgttaaa agagaccatc aatgaggaag
     2401 ctgcagaatg ggatagagtg catccagtgc atgcagggcc tattgcacca ggccagatga
     2461 gagaaccaag gggaagtgac atagcaggaa ctactagtac ccttcaggaa caaataggat
     2521 ggatgacaca taatccacct atcccagtag gagaaatcta taaaagatgg ataatcctgg
     2581 gattaaataa aatagtaaga atgtatagcc ctaccagcat tctggacata agacaaggac
     2641 caaaggaacc ctttagagac tatgtagacc gattctataa aactctaaga gccgagcaag
     2701 cttcacaaga ggtaaaaaat tggatgacag aaaccttgtt ggtccaaaat gcgaacccag
     2761 attgtaagac tattttaaaa gcattgggac caggagcgac actagaagaa atgatgacag
     2821 catgtcaggg agtgggggga cccggccata aagcaagagt tttggctgaa gcaatgagcc
     2881 aagtaacaaa tccagctacc ataatgatac agaaaggcaa ttttaggaac caaagaaaga
     2941 ctgttaagtg tttcaattgt ggcaaagaag ggcacatagc caaaaattgc agggccccta
     3001 ggaaaaaggg ctgttggaaa tgtggaaagg aaggacacca aatgaaagat tgttccggtg
     3061 gaggtggatc cggtggaggt tccatggcgt ccaatttcac gcagttcgtc ctggttgaca
     3121 acggggggac tggggacgtt acggtcgctc cgagcaactt tgccaatggt attgcggagt
     3181 ggatttcttc taattcacgg tcccaagctt acaaagtgac ctgttccgtg cggcaaagtt
     3241 ctgctcagaa tagaaagtac actataaagg tcgaagtccc taagggggcc tggcgatcat
     3301 atctcaatat ggagcttacc atcccaatat ttgccactaa ttctgattgt gaattgattg
     3361 tcaaagcaat gcaaggactc ttgaaagacg gaaacccaat ccccagcgca atcgcagcca
     3421 actccggtat atactccgga ggtggaggtg gaactgagag acaggctaat tttttaggga
     3481 agatctggcc ttcccacaag ggaaggccag ggaattttct tcagagcaga ccagagccaa
     3541 cagccccacc agaagagagc ttcaggtttg gggaagagac aacaactccc tctcagaagc
     3601 aggagccgat agacaaggaa ctgtatcctt tagcttccct cagatcactc tttggcagcg
     3661 acccctcgtc acaataaaga taggggggca attaaaggaa gctctattag atacaggagc
     3721 agatgataca gtattagaag aaatgaattt gccaggaaga tggaaaccaa aaatgatagg
     3781 gggaattgga ggttttatca aagtaagaca gtatgatcag atactcatag aaatctgcgg
     3841 acataaagct ataggtacag tattagtagg acctacacct gtcaacataa ttggaagaaa
     3901 tctgttgact cagattggct gcactttaaa ttttcccatt agtcctattg agactgtacc
     3961 agtaaaatta aagccaggaa tggatggccc aaaagttaaa caatggccat tgacagaaga
     4021 aaaaataaaa gcattagtag aaatttgtac agaaatggaa aaggaaggaa aaatttcaaa
     4081 aattgggcct gaaaatccat acaatactcc agtatttgcc ataaagaaaa aagacagtac
     4141 taaatggaga aaattagtag atttcagaga acttaataag agaactcaag atttctggga
     4201 agttcaatta ggaataccac atcctgcagg gttaaaacag aaaaaatcag taacagtact
     4261 ggatgtgggc gatgcatatt tttcagttcc cttagataaa gacttcagga agtatactgc
     4321 atttaccata cctagtataa acaatgagac accagggatt agatatcagt acaatgtgct
     4381 tccacaggga tggaaaggat caccagcaat attccagtgt agcatgacaa aaatcttaga
     4441 gccttttaga aaacaaaatc cagacatagt catctatcaa tacatggatg atttgtatgt
     4501 aggatctgac ttagaaatag ggcagcatag aacaaaaata gaggaactga gacaacatct
     4561 gttgaggtgg ggatttacca caccagacaa aaaacatcag aaagaacctc cattcctttg
     4621 gatgggttat gaactccatc ctgataaatg gacagtacag cctatagtgc tgccagaaaa
     4681 ggacagctgg actgtcaatg acatacagaa attagtggga aaattgaatt gggcaagtca
     4741 gatttatgca gggattaaag taaggcaatt atgtaaactt cttaggggaa ccaaagcact
     4801 aacagaagta gtaccactaa cagaagaagc agagctagaa ctggcagaaa acagggagat
     4861 tctaaaagaa ccggtacatg gagtgtatta tgacccatca aaagacttaa tagcagaaat
     4921 acagaagcag gggcaaggcc aatggacata tcaaatttat caagagccat ttaaaaatct
     4981 gaaaacagga aagtatgcaa gaatgaaggg tgcccacact aatgatgtga aacaattaac
     5041 agaggcagta caaaaaatag ccacagaaag catagtaata tggggaaaga ctcctaaatt
     5101 taaattaccc atacaaaagg aaacatggga agcatggtgg acagagtatt ggcaagccac
     5161 ctggattcct gagtgggagt ttgtcaatac ccctccctta gtgaagttat ggtaccagtt
     5221 agagaaagaa cccataatag gagcagaaac tttctatgta gatggggcag ccaataggga
     5281 aactaaatta ggaaaagcag gatatgtaac tgacagagga agacaaaaag ttgtccccct
     5341 aacggacaca acaaatcaga agactgagtt acaagcaatt catctagctt tgcaggattc
     5401 gggattagaa gtaaacatag tgacagactc acaatatgca ttgggaatca ttcaagcaca
     5461 accagataag agtgaatcag agttagtcag tcaaataata gagcagttaa taaaaaagga
     5521 aaaagtctac ctggcatggg taccagcaca caaaggaatt ggaggaaatg aacaagtaga
     5581 taaattggtc agtgctggaa tcaggaaagt actattttta gatggaatag ataaggccca
     5641 agaagaacat gagaaatatc acagtaattg gagagcaatg gctagtgatt ttaacctacc
     5701 acctgtagta gcaaaagaaa tagtagccag ctgtgataaa tgtcagctaa aaggggaagc
     5761 catgcatgga caagtagact gtagcccagg aatatggcag ctagtttgta cacatttaga
     5821 aggaaaagtt atcttggtag cagttcatgt agccagtgga tatatagaag cagaagtaat
     5881 tccagcagag acagggcaag aaacagcata cttcctctta aaattagcag gaagatggcc
     5941 agtaaaaaca gtacatacag acaatggcag caatttcacc agtactacag ttaaggccgc
     6001 ctgttggtgg gcggggatca agcaggaatt tggcattccc tacaatcccc aaagtcaagg
     6061 agtaatagaa tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc
     6121 tgaacatctt aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg
     6181 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
     6241 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
     6301 cagcagagat ccagtttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt
     6361 agtaatacaa gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcatcag
     6421 ggattatgga aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta
     6481 acacatggaa ttctgcaaca actgctgttt atccatttca gaattgggtg tcgacatagc
     6541 agaataggcg ttactcgaca gaggagagca agaaatggag ccagtagatc ctagactaga
     6601 gccctggaag catccaggaa gtcagcctaa aactgcttgt accaattgct attgtaaaaa
     6661 gtgttgcttt cattgccaag tttgtttcat gacaaaagcc ttaggcatct cctatggcag
     6721 gaagaagcgg agacagcgac gaagagctca tcagaacagt cagactcatc aagcttctct
     6781 atcaaagcag taagtagtac atgtaatgca acctataata gtagcaatag tagcattagt
     6841 agtagcaata ataatagcaa tagttgtgtg gtccatagta atcatagaat ataggaaaat
     6901 ggccgctgat cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat
     6961 ataaatataa agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa
     7021 gagtggtgca gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg
     7081 gagcagcagg aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat
     7141 tattgtctgg tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc
     7201 atctgttgca actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg
     7261 aaagatacct aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt
     7321 gcaccactgc tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga
     7381 atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact
     7441 ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag
     7501 ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat
     7561 tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta
     7621 tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc
     7681 cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca
     7741 gatccattcg attagtgaac ggatccttgg cacttatctg ggacgatctg cggagcctgt
     7801 gcctcttcag ctaccaccgc ttgagagact tactcttgat tgtaacgagg attgtggaac
     7861 ttctgggacg cagggggtgg gaagccctca aatattggtg gaatctccta caatattgga
     7921 gtcaggagct aaagaatagt gctgttagct tgctcaatgc cacagccata gcagtagctg
     7981 aggggacaga tagggttata gaagtagtac aaggagcttg tagagctatt cgccacatac
     8041 ctagaagaat aagacagggc ttggaaagga ttttgctata agctcgaaac aaccggtacc
     8101 tctagaacta tagctagcag atctttttcc ctctgccaaa aattatgggg acatcatgaa
     8161 gccccttgag catctgactt ctggctaata aaggaaattt attttcattg caatagtgtg
     8221 ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc atttaaaaca
     8281 tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg ctgccatgaa
     8341 caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg tccattcctt
     8401 attccataga aaagccttga cttgaggtta gatttttttt atattttgtt ttgtgttatt
     8461 tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat ttttcctcct
     8521 ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc tcgacctgca
     8581 gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     8641 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     8701 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     8761 cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     8821 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     8881 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     8941 aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc agcttataat
     9001 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     9061 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat ccgctgcatt
     9121 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     9181 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     9241 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     9301 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     9361 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     9421 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     9481 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     9541 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     9601 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     9661 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     9721 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     9781 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     9841 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     9901 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     9961 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
    10021 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
    10081 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
    10141 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
    10201 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
    10261 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
    10321 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
    10381 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
    10441 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
    10501 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
    10561 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
    10621 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
    10681 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
    10741 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
    10801 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
    10861 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
    10921 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
    10981 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
    11041 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
    11101 ggtcgaca
//
