LOCUS       prHom-1.        5698 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Mammalian vector encoding two FKBP homodimerizer domains, for 
            creating cytoplasmic oligomers that can be dissociated with a drug. 
            Also known as pC4EN-FM2E.
ACCESSION   .
VERSION     .
KEYWORDS    prHom-1
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5698)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5698)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Clone into the XbaI site to fuse two copies of DmrD to the 
            C-terminus of the partner protein.
FEATURES             Location/Qualifiers
     source          1..5698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        19..322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        323..526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     5'UTR           616..655
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             676..678
                     /codon_start=1
                     /product="start codon"
                     /label=start codon
                     /note="ATG"
                     /translation="M"
     CDS             688..1008
                     /label=DmrD
                     /note="dimeric F36M mutant of FK506-binding protein FKBP12"
     CDS             1015..1335
                     /codon_start=1
                     /product="dimeric F36M mutant of FK506-binding protein
                     FKBP12"
                     /label=dimeric F36M mutant of FK506-binding protein
                     FK
                     /note="DmrD"
                     /note="dimerization can be reversed by ligand binding"
                     /translation="GVQVETISPGDGRTFPKRGQTCVVHYTGMLEDGKKMDSSRDRNKP
                     FKFMLGKQEVIRGWEEGVAQMSVGQRAKLTISPDYAYGATGHPGIIPPHATLVFDVELL
                     KLE"
     CDS             1342..1368
                     /label=HA
                     /note="HA (human influenza hemagglutinin) epitope tag"
     intron          1400..1972
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     polyA_signal    2169..2224
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     rep_origin      complement(2577..2712)
                     /direction=LEFT
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      complement(2989..3577)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3748..4620)
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=beta-lactamase
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFQVEVAMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDL
                     VEYSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTR
                     LDRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAE
                     IGASLIKHW"
     promoter        complement(4621..4725)
                     /label=AmpR promoter
     rep_origin      complement(5057..5512)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     5657..5673
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        5680..5698
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acgcgttcga gctcgccccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
       61 caacgacccc cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg
      121 gactttccat tgacgtcaat gggtggagta tttacggtaa actgcccact tggcagtaca
      181 tcaagtgtat catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc
      241 ctggcattat gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt
      301 attagtcatc gctattacca tggtgatgcg gttttggcag tacatcaatg ggcgtggata
      361 gcggtttgac tcacggggat ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt
      421 ttggcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
      481 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
      541 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
      601 atccagcctc cgggggatct tggtggcgtg aaactcccgc acctcttcgg ccagcgaatt
      661 ccagaagccg ccaccatggc ttctagagga gtgcaggtgg agactatctc cccaggagac
      721 gggcgcacct tccccaagcg cggccagacc tgcgtggtgc actacaccgg gatgcttgaa
      781 gatggaaaga aaatggattc ctcccgggac agaaacaagc cctttaagtt tatgctaggc
      841 aagcaggagg tgatccgagg ctgggaagaa ggggttgccc agatgagtgt gggtcagaga
      901 gccaaactga ctatatctcc agattatgcc tatggtgcca ctgggcaccc aggcatcatc
      961 ccaccacatg ccactctcgt cttcgatgtg gagcttctaa aactggaaac tagaggagtg
     1021 caggtggaga ctatctcccc aggagacggg cgcaccttcc ccaagcgcgg ccagacctgc
     1081 gtggtgcact acaccgggat gcttgaagat ggaaagaaaa tggattcctc ccgggacaga
     1141 aacaagccct ttaagtttat gctaggcaag caggaggtga tccgaggctg ggaagaaggg
     1201 gttgcccaga tgagtgtggg tcagagagcc aaactgacta tatctccaga ttatgcctat
     1261 ggtgccactg ggcacccagg catcatccca ccacatgcca ctctcgtctt cgatgtggag
     1321 cttctaaaac tggaaactag ttatccgtac gacgtaccag actacgcata agaaaagtga
     1381 ggatcctgag aacttcaggg tgagtttggg gacccttgat tgttctttct ttttcgctat
     1441 tgtaaaattc atgttatatg gagggggcaa agttttcagg gtgttgttta gaatgggaag
     1501 atgtcccttg tatcaccatg gaccctcatg ataattttgt ttctttcact ttctactctg
     1561 ttgacaacca ttgtctcctc ttattttctt ttcattttct gtaacttttt cgttaaactt
     1621 tagcttgcat ttgtaacgaa tttttaaatt cacttttgtt tatttgtcag attgtaagta
     1681 ctttctctaa tcactttttt ttcaaggcaa tcagggtata ttatattgta cttcagcaca
     1741 gttttagaga acaattgtta taattaaatg ataaggtaga atatttctgc atataaattc
     1801 tggctggcgt ggaaatattc ttattggtag aaacaactac atcctggtca tcatcctgcc
     1861 tttctcttta tggttacaat gatatacact gtttgagatg aggataaaat actctgagtc
     1921 caaaccgggc ccctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     1981 caacgtgctg gttgttgtgc tgtctcatca ttttggcaaa ggattcactc ctcaggtgca
     2041 ggctgcctat cagaaggtgg tggctggtgt ggccaatgcc ctggctcaca aataccactg
     2101 agatcttttt ccctctgcca aaaattatgg ggacatcatg aagccccttg agcatctgac
     2161 ttctggctaa taaaggaaat ttattttcat tgcaatagtg tgttggaatt ttttgtgtct
     2221 ctcactcgga aggacatatg ggagggcaaa tcatttaaaa catcagaatg agtatttggt
     2281 ttagagtttg gcaacatatg ccatatgctg gctgccatga acaaaggtgg ctataaagag
     2341 gtcatcagta tatgaaacag ccccctgctg tccattcctt attccataga aaagccttga
     2401 cttgaggtta gatttttttt atattttgtt ttgtgttatt tttttcttta acatccctaa
     2461 aattttcctt acatgtttta ctagccagat ttttcctcct ctcctgacta ctcccagtca
     2521 tagctgtccc tcttctctta tgaagatccc tcgaggagct ttttgcaaaa gccctaggcc
     2581 tccaaaaaag cctcttcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     2641 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     2701 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgctgcatta
     2761 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     2821 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     2881 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     2941 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3001 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3061 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3121 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3181 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3241 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3301 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     3361 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     3421 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     3481 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     3541 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     3601 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     3661 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     3721 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     3781 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     3841 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     3901 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     3961 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4021 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4081 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4141 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4201 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4261 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4321 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     4381 gccacatagc agaactttaa aagtgctcat cattgccacc tccacctgaa aacgttcttc
     4441 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     4501 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     4561 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     4621 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     4681 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     4741 agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg
     4801 tatcacgagg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
     4861 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
     4921 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
     4981 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
     5041 aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt
     5101 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     5161 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     5221 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     5281 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     5341 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     5401 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     5461 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat
     5521 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta
     5581 cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt
     5641 tcccagtcac gacgttgtaa aacgacggcc agtgaattgt aatacgactc actatagg
//
