LOCUS       62056_18635        6908 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6908)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..6908
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             230..250
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             287..328
                     /codon_start=1
                     /label=V5 tag
                     /note="epitope tag from simian virus 5"
                     /translation="GKPIPNPLLGLDST"
     misc_feature    371..957
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             968..1990
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     polyA_signal    2018..2242
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2289..2305)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(2313..2329)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2337..2367)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2382..2403)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2461..2657
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     polyA_signal    2663..2797
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(3036..3624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3798..4655)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     intron          6055..6908
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and
                     rabbit beta-globin"
ORIGIN
        1 gccgcgccgc cgtccccttc tccctctcca gcctcggggc tgtccgcggg gggacggctg
       61 ccttcggggg ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag
      121 agcctctgct aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct
      181 ggttattgtg ctgtctcatc attttggcaa agaattcctc gagaccatgc ccaagaagaa
      241 gaggaaggtg gctagcgcta agatctcatg cccgggatcc accggtggta agcctatccc
      301 taaccctctc ctcggtctcg attctacgtg agcggccgct cgataagctt gatatcgaat
      361 tccgcccccc cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
      421 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
      481 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
      541 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
      601 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
      661 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
      721 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
      781 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
      841 ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
      901 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc cacaacccaa
      961 cccaaagatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa
     1021 gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag
     1081 cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta
     1141 caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct
     1201 tgacattggg gaattcagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt
     1261 cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc
     1321 catggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc
     1381 gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca
     1441 tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct
     1501 cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga
     1561 tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag
     1621 cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg
     1681 gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg
     1741 atcgccgcgg ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt
     1801 ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg
     1861 atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac
     1921 cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag
     1981 ggcaaaggaa tagtcggagc tcgctgatca gcctcgactg tgccttctag ttgccagcca
     2041 tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc
     2101 ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg
     2161 gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct
     2221 ggggatgcgg tgggctctat ggcttctgag gcggaaagaa cctgcagccc aagcttggcg
     2281 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     2341 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     2401 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     2461 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2521 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2581 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2641 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag
     2701 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     2761 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa
     2821 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     2881 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     2941 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     3001 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     3061 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     3121 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     3181 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     3241 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     3301 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     3361 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     3421 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     3481 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     3541 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     3601 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     3661 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     3721 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     3781 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     3841 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     3901 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     3961 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     4021 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     4081 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     4141 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     4201 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     4261 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     4321 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     4381 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     4441 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     4501 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     4561 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     4621 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atgaccgctt
     4681 tggaaaaaca aagactgtat ttcctggaaa ttaatgttta ttcaataaac tgtgtattca
     4741 gctatattca catagtggtg aggctgaaat gaggcgggaa gaggcggttg gggcttaatt
     4801 atatcaattt gggtggcccc acagcgcctc caaggcgcca gtcctgtttt gacaagttgc
     4861 ctctggaagc ctctcttctt tttctccaga gtaagcggag gccaggggcc cccggcctct
     4921 gcttaatact aaaaaaaaca gctgttgtca tagtaatgat tgggtggaaa cattccaggc
     4981 ctgggtggag aggctttttg cttcctcttg caaaaccaca ctgacattcc aggcctgggt
     5041 ggagaggctt tttgcttcct cttgcaaaac cacactgccc tctggagggc agttgcctag
     5101 caactaatta aaagaggatg tcgcacggcc agctgcggtc agttagtcac ttcctgctta
     5161 actgacttga cattttctat tttaagagtc gggaggaaaa ttactgtgtt ggaggccctc
     5221 cgccatcttc tgaagctgaa tcgaattaac ttgtttattg cagcttataa tggttacaaa
     5281 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5341 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatatca tcgtcgacat
     5401 tgattattga ctagttatta atagtaatca attacggggt cattagttca tagcccatat
     5461 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     5521 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     5581 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     5641 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     5701 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     5761 atcgctatta ccatggtcga ggtgagcccc acgttctgct tcactctccc catctccccc
     5821 ccctccccac ccccaatttt gtatttattt attttttaat tattttgtgc agcgatgggg
     5881 gcgggggggg ggggggggcg cgcgccaggg gggggggggg gggggggggg gggggggggg
     5941 gggggggggg ggggggcggc ggcagccaat cagagcggcg cgctccgaaa gtttcctttt
     6001 atggcgaggc ggcggcggcg gcggccctat aaaaagcgaa gcgcgcggcg ggcgggagtc
     6061 gctgcgcgct gccttcgccc cgtgccccgc tccgccgccg cctcgcgccg cccgccccgg
     6121 ctctgactga ccgcgttact cccacaggtg agcgggcggg acggcccttc tcctccgggc
     6181 tgtaattagc gcttggttta atgacggctt gtttcttttc tgtggctgcg tgaaagcctt
     6241 gaggggctcc gggagggccc tttgtgcggg gggagcggct cggggggtgc gtgcgtgtgt
     6301 gtgtgcgtgg ggagcgccgc gtgcggctcc gcgctgcccg gcggctgtga gcgctgcggg
     6361 cgcggcgcgg ggctttgtgc gctccgcagt gtgcgcgagg ggagcgcggc cgggggcggt
     6421 gccccgcggt gcgggggggg ctgcgagggg aacaaaggct gcgtgcgggg tgtgtgcgtg
     6481 ggggggtgag cagggggtgt gggcgcgtcg gtcgggctgc aaccccccct gcacccccct
     6541 ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtacggg gcgtggcgcg
     6601 gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg ggcggggccg
     6661 cctcgggccg gggagggctc gggggagggg cgcggcggcc cccggagcgc cggcggctgt
     6721 cgaggcgcgg cgagccgcag ccattgcctt ttatggtaat cgtgcgagag ggcgcaggga
     6781 cttcctttgt cccaaatctg tgcggagccg aaatctggga ggcgccgccg caccccctct
     6841 agcgggcgcg gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     6901 cgtgcgtc
//
