LOCUS       V016409                 5036 bp    DNA     circular SYN 12-MAY-2020
DEFINITION  Exported.
ACCESSION   V016409
VERSION     V016409
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5036)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
   PUBMED   32341562
REFERENCE   2  (bases 1 to 5036)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 5036)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways,
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
FEATURES             Location/Qualifiers
     source          1..5036
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1634
                     /gene="npgA"
                     /label="4'-phosphopantetheinyl transferase NpgA"
                     /note="4'-phosphopantetheinyl transferase NpgA from
                     Emericella nidulans (strain FGSC A4 / ATCC 38163 / CBS
                     112.46 / NRRL 194 / M139). Accession#: G5EB87"
     misc_feature    1644..1700
                     /label="MCS"
                     /note="multiple cloning site"
     polyA_signal    1824..1945
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1952..2407)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2434..2538
                     /label="AmpR promoter"
     promoter        2540..2897
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             2932..3723
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3958..4005
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4334..4922
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      601 ccatggtcca agacacctca agcgctagta ccagtccaat tttgacaaga tggtatatag
      661 atactagacc tttgactgct tctaccgcag ccttgccact gttggaaact ctgcagcctg
      721 cagaccagat aagtgtccaa aagtactacc atctgaagga taagcatatg tctctcgcat
      781 ccaatcttct caagtacttg tttgttcaca ggaattgccg aatcccatgg tctagcattg
      841 taatatcaag aactccagat ccccatagaa ggccctgtta tataccaccc tctggttctc
      901 aagaggacag ctttaaggat ggctacactg gaattaatgt cgagttcaac gtatcccatc
      961 aagcaagcat ggtggcaata gcaggcactg catttacacc caactctgga ggtgattcca
     1021 aacttaagcc agaggttggc atagacatta cttgcgtgaa tgagcgacag gggcgaaacg
     1081 gtgaggagcg atcccttgaa agtttgaggc agtacataga tattttctca gaggtttttt
     1141 ccaccgcaga gatggcaaac atcagaagat tggatggggt gtcatcaagc tccttgagcg
     1201 ccgataggtt ggtggactat ggttaccgat tgttttacac ttattgggct cttaaggaag
     1261 catacatcaa gatgacaggg gaagccctgc tggcaccatg gctcagggag ttggaattta
     1321 gtaatgtcgt tgccccagca gcagtggccg aatccggaga ttccgccgga gactttggag
     1381 agccttatac cggggtacga acaacccttt acaaaaactt ggtcgaggat gttaggatag
     1441 aggtggctgc tctgggaggt gactacttgt tcgccactgc tgcacgaggg ggcggtatag
     1501 gtgcctccag ccgaccaggg ggaggccctg atggctcagg aattaggagt caagatccct
     1561 ggcgaccatt taaaaagctc gacatagaga gagacataca gccttgcgcc actggagtct
     1621 gtaattgtct gtctcgaggt tgaagatctc gagctcaagc ttcgaattct gcagtcgacg
     1681 gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc cataccacat
     1741 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     1801 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     1861 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1921 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     1981 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     2041 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     2101 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2161 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2221 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2281 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2341 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2401 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2461 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2521 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     2581 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2641 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2701 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2761 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2821 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2881 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     2941 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     3001 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     3061 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     3121 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3181 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3241 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3301 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3361 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3421 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     3481 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3541 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3601 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3661 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3721 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     3781 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     3841 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     3901 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     3961 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     4021 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     4081 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     4141 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     4201 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4261 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4321 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4381 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4441 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4501 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4561 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4621 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4681 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4741 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4801 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4861 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4921 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4981 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
