LOCUS       62056_18170        9092 bp DNA     circular SYN 12-MAY-2020
DEFINITION  Cloning vector pnnHispS-C1, complete sequence.
ACCESSION   MT233537
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9092)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
  PUBMED    32341562
REFERENCE   2  (bases 1 to 9092)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic 
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 9092)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways, 
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9092
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     gene            603..5699
                     /gene="hispS"
                     /label=hispS
     CDS             603..5699
                     /codon_start=1
                     /transl_table=11
                     /gene="hispS"
                     /product="HispS"
                     /label=hispS
                     /note="derived from N. nambi"
                     /protein_id="QJQ48095.1"
                     /translation="MNSSKNPPSTLLDVFLDTARNLDTASRNVLECGEHRWSYRELDTV
                     SSALAQHLRYTVGLSPTVAVISENHPYILALMLAVWKLGGTFAPIDVHSPAELVAGMLN
                     IVSPSCLVIPSSDVTNQTLACDLNIPVVAFHPHQSTIPELNKKYLTDSQISPDLPFPDP
                     NRPALYLFTSSATSRSNLKCVPLTHTFILRNSLSKRAWCKRMRPETDFDGIRVLGWAPW
                     SHVLAHMQDIGPLTLLNAGCYVFATTPSTYPTELKDDRDVISCAANAVMYKGVKSFACL
                     PFVLGGLKALCESEPSVKAQLQVEERAQLLKSLQHMKILECGGAMLEVSVASWAIENRI
                     PISIGIGMTETGGALFAGPVQAIQTGFSSEDKFIEDATYLLVKDDYESHAEEDINEGEL
                     VVKSRMLPRGYLGYNDPSFSVDDAGWVTFKTGDRYSVTPDGKFSWLGRNTDFIQMTSGE
                     TLDPRPIESLLCESSLISRACVIGDKFLNGPATAVCAIIELEPTTVEKGQAHSRDIARI
                     FAPINRDLPPPLRIAWSHVLVLQPSEKIPMTKKGTIFRKKIEQVFGSALGGSSGDNSQA
                     TTDASVVRRDELSNTVKHIISRVLGVSDDELLWTLSFAELGMTSALATRIANELNEVLV
                     GVNLPINACYIHVDLPSLSNAVYAKLAHLKLPDRTPEPRKAPVENPGGKEIVIVGQAFR
                     LPGSINDVASLRDAFLARQASSIITEIPPDRWDHASFYPKDIRFNKAGLVDIANYDHSF
                     FGLTATEALYLSPTMRLALEVSFEALENANIPVSQLKGSQTAVYVATTDDGFETLLNAE
                     AGYDAYTRFYGTGRAASTASGRISYLLDVHGPSITVDTACSGGAVCIDQAIDYLQSSSA
                     ADTAIICASNTHCWPGSFMFLSAQGMVSSGGRCATFTTDADGYVPSEGAVAFILKTREA
                     AMRDKDTILATIKATQISHNGRSQGLVAPNVNSQADLHRSLLQKAGLSPADIHFIEAHG
                     TGTSLGDLSEIQAINDAYTSSQPRTAGPLIVSASKTVIGHTEPAGPLVGMLSVLNSFKE
                     GAVPGLAHLTADNLNPALDCSSVPLLIPYQPVHLAAPKPHRAAVMSYGFSGTLGGIVLE
                     APDEERLEEEPPNDKPMLFVVSAKTHTALIEYLGRYLEFLLQANPQDFCDICYTSCVGR
                     EHYRYRFACVANDMEDLIGQLQKRLGSKVPPKPSYKRGALAFAFSGQGTQFRGMATELA
                     KAYSGFRKIVSDLAKRASELSGHAIDRFLLAYDIGAENVAPDSEADQICIFVYQCSVLR
                     WLQTMGIRPSAVIGHSLGEISASVAAGALSLDSALDLVISRARLLRSSTNAPAGMAAMS
                     ASQDEVVELIGKLDLDKANSLSVSVINGPQNTVVSGSSAAIESIVALAKGRKIKASALN
                     INQAFHSPYVDSAVPGLRAWSEKHISSARPLQIPLYSTLLGAQVSEGQMLNPDHWVDHA
                     RKPVQFAQAATIMKESFTGVIIDIGPQVVAWSLLLSNGLTSVTALAAKRGRSQQVAFLS
                     ALADLYQDYGVVPDFVGLYAQQEDASRLKKTDILTYPFQRVRRYPSFIPSRRAPTHAHV
                     QDEETLSSGSSTPTLENTDLDSGKESLMGPTRGLLRVDDLRDSIVSSVKDVLELKSNED
                     LDLSESLNALGMDSIMFAQLRKRIGEGLGLSVPMVFLSDAFSIGEMVSNLVEQAEASED
                     N"
     misc_feature    5700..5756
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    5880..6001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(6008..6463)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        6490..6594
                     /label=AmpR promoter
     promoter        6596..6953
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             6988..7779
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    8014..8061
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      8390..8978
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      601 ccatgaactc cagtaaaaac cccccttcaa ccctactgga cgtcttcttg gacacggcaa
      661 ggaacttgga taccgcttca cgtaacgtac tagagtgtgg tgaacaccgt tggagttatc
      721 gtgagctaga cactgtgtcc tccgctttag ctcagcatct aagatataca gtaggtctgt
      781 cccccacagt ggcagtgatc tcagaaaatc atccctatat attagcattg atgttggccg
      841 tgtggaagct aggcggtaca tttgcaccta ttgatgttca ttcacccgct gaattggtgg
      901 ctggcatgct taacattgta tccccttctt gcttagtaat cccatcttca gacgtaacaa
      961 accaaacact agcctgcgac ctgaacatcc ctgtagttgc ctttcaccca catcagtcaa
     1021 ctattcccga gctgaataag aaatatttaa ccgactccca aatcagtcca gatttgccat
     1081 ttccagaccc aaacaggcca gccctatacc tatttacgtc ttcagcaacc agtcgtagta
     1141 acttgaaatg tgttccctta acgcatacat tcattcttag gaactctctt tcaaagcgtg
     1201 cctggtgcaa gcgtatgagg ccagagaccg actttgacgg tataagagtg ttaggatggg
     1261 ccccttggtc ccacgtgctg gctcacatgc aagacatcgg cccattaacc cttcttaatg
     1321 ctggttgtta tgtatttgcc actacgccat caacatatcc cacggaactg aaggacgacc
     1381 gtgacgtgat ttcttgtgct gcaaacgccg tgatgtacaa gggagtgaag tcattcgcct
     1441 gtcttccatt tgtactaggc ggactgaagg ccttgtgcga gtccgaacct agtgtgaaag
     1501 ctcagttgca agtggaggaa cgtgcccaac tgttgaaatc tcttcaacac atgaagattt
     1561 tagagtgtgg cggcgccatg ttagaggttt ctgtggcaag ttgggccatc gagaatagga
     1621 tcccaatatc catcggaatc ggcatgaccg agaccggagg agctctgttt gctggacccg
     1681 ttcaagcaat ccaaacgggt ttcagtagtg aggataagtt catagaagac gcaacctact
     1741 tattggtaaa ggacgactac gagtcacacg ctgaagaaga cataaacgaa ggagaacttg
     1801 tcgtgaaatc ccgtatgtta ccccgtggtt atcttggcta caatgaccca tccttctcag
     1861 tggacgatgc tggatgggta actttcaaaa cgggagacag gtactcagtt acccccgacg
     1921 gaaaattttc atggctaggt aggaacacgg atttcatcca gatgacatcc ggcgagactc
     1981 tggatcccag gccaatcgag tctctactat gcgagtcatc tttgatttcc cgtgcctgcg
     2041 tcataggcga caagtttcta aacggccctg ccaccgccgt atgtgctatc attgaattag
     2101 aaccaacgac cgtcgaaaag ggacaagcac attcacgtga tatagcaaga attttcgcac
     2161 ctatcaaccg tgacttacca ccccccctac gtattgcttg gtcacatgtt cttgtcctac
     2221 aacctagtga aaagataccc atgactaaaa aaggaacaat cttcaggaaa aagatagaac
     2281 aagttttcgg atccgctctt ggtggatcct caggcgacaa tagtcaagcc accaccgacg
     2341 cttcagtagt taggagagac gagctttcaa atacagtaaa gcacataata tccagggttt
     2401 taggagtatc agacgatgag ttgctgtgga ccctttcttt cgcagagctt ggaatgactt
     2461 ccgcactggc aacgagaatt gccaatgaat tgaacgaggt tcttgtgggc gttaacctac
     2521 ctatcaacgc ttgttacatc cacgttgatt tacctagtct tagtaatgca gtatatgcaa
     2581 agttggctca ccttaagcta cccgatagaa ctcctgaacc caggaaagca cccgtggaga
     2641 atccaggtgg taaagaaatt gttattgtag gccaggcttt taggttacca ggatcaataa
     2701 acgacgtcgc ttcccttaga gacgcattcc tagctagaca agccagtagt ataataacgg
     2761 aaataccccc tgatagatgg gaccatgcct cattttaccc taaagacata agattcaata
     2821 aagcaggact tgttgacatc gctaattatg atcatagttt cttcggtcta acggcaaccg
     2881 aggcattgta cttatcacca acaatgcgtt tggcattaga ggtttctttc gaggccctgg
     2941 agaacgcaaa cattccagtc tcccagctta agggttctca aactgctgta tacgttgcca
     3001 caacggacga tggatttgaa acgctgctga atgccgaagc tggatatgac gcatatacga
     3061 gattctatgg cacaggacgt gctgccagta ccgccagtgg cagaatttca tacctgctgg
     3121 atgtccacgg tccctcaata actgtcgaca ctgcttgttc tggtggcgca gtatgtatcg
     3181 accaggctat agattacctg caatcttcca gtgccgctga tacggcaatt atatgcgcat
     3241 ccaataccca ctgctggcca ggctcattta tgttcttatc cgcacaaggt atggtgtcaa
     3301 gtggtggtag atgcgcaact tttaccaccg atgctgacgg atacgttcct tccgaaggtg
     3361 ctgtagcatt catcttaaag acaagagaag cagccatgag agacaaggac actattttgg
     3421 ctacgattaa ggcaactcag atatcccaca atggccgttc ccagggtctt gtcgcaccta
     3481 acgtcaattc tcaggcagac ctgcacaggt cattattgca aaaggctgga ttatcaccag
     3541 ccgatatcca cttcatagag gctcacggca caggtaccag tcttggcgat ttatcagaaa
     3601 ttcaagctat caatgacgca tatacttcct cccaaccccg tactgctggt ccacttatag
     3661 tttcagctag taagacagtt attggacata cggaacccgc cggcccattg gtaggaatgt
     3721 tgtccgtatt aaactccttc aaagagggag ccgttcccgg cctagcccat cttaccgccg
     3781 acaacctaaa tcctgcatta gattgctcaa gtgttcctct tctgataccc tatcaacccg
     3841 ttcatcttgc tgcacccaaa cctcatagag ctgcagtgat gtcctatgga tttagtggaa
     3901 ctcttggcgg tatcgtcctt gaagctccag acgaagaacg tcttgaagag gagcccccta
     3961 acgataagcc aatgttattc gtcgtaagtg ctaaaactca cacagccctt atcgagtacc
     4021 ttggaagata cctggaattc ttgctgcaag caaatccaca agatttctgt gacatctgtt
     4081 atacttcctg tgttggtaga gaacactata ggtacagatt cgcttgcgtt gctaatgata
     4141 tggaggacct gattggacaa ctgcagaaac gtctgggctc caaagtccca cccaagccta
     4201 gttacaagag gggagcctta gcatttgctt tctctggtca aggaactcaa ttcaggggaa
     4261 tggccacgga gcttgctaaa gcatactcag gctttaggaa gatcgtctct gatttggcaa
     4321 agcgtgcctc cgaattatcc ggccacgcca ttgataggtt cctgcttgcc tatgatatcg
     4381 gcgccgagaa tgtcgcccct gatagtgaag cagatcagat ttgtattttc gtctatcaat
     4441 gcagtgtcct gcgttggtta cagactatgg gcataaggcc tagtgcagta ataggccaca
     4501 gtttaggcga aatctctgca tcagtagccg caggtgcact aagtctagat tctgctctag
     4561 atctggtaat atccagagct agacttttaa gaagttcaac gaacgctccc gcaggtatgg
     4621 cagccatgtc agctagtcag gacgaggtag tagagctaat aggaaagtta gatttggata
     4681 aggcaaactc attaagtgtc agtgtgataa acggtcccca gaacacggtg gtcagtggat
     4741 cctctgccgc catcgaatct atagtcgctc tagcaaaagg tagaaaaatt aaggcctctg
     4801 ccctaaacat aaatcaggct ttccactcac cttacgttga ttcagcagtc cccggtctaa
     4861 gggcctggtc agagaagcat atatcatccg cccgtcccct tcagatcccc ctgtattcaa
     4921 ccttgttggg cgcccaggtg agtgaaggac agatgttgaa ccctgaccat tgggtagacc
     4981 atgctagaaa gccagttcaa ttcgcacaag ctgccacgat aatgaaagag agttttactg
     5041 gagtcatcat tgacataggt cctcaagtag tcgcttggtc cttgttgcta tcaaatggac
     5101 ttacgtctgt caccgcactg gccgcaaaaa gaggacgtag tcagcaagta gcattcttaa
     5161 gtgcacttgc tgacctgtat caggactacg gcgtagttcc cgacttcgtc ggcctatatg
     5221 ctcagcaaga ggacgcaagt aggttaaaga agacggatat tcttacatac ccattccaga
     5281 gagttaggcg ttatccttct ttcattccat caaggagagc accaactcat gcacatgtgc
     5341 aggatgaaga aactctaagt agtggtagtt ccaccccaac cctagaaaat acggacttag
     5401 actccggcaa ggaatcccta atgggtccta cccgtggatt gctaagggta gatgatctac
     5461 gtgattccat tgtatcctca gtaaaagatg ttttagaatt gaaatccaac gaagatctag
     5521 accttagtga gtcattgaat gctttaggca tggattcaat aatgtttgct caacttagga
     5581 aaagaatcgg tgaaggtttg ggattgtcag tgccaatggt cttcttgtca gacgcattca
     5641 gtatcggaga gatggtgtca aatcttgtgg aacaggccga agctagtgag gacaactgaa
     5701 gatctcgagc tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg
     5761 gatctagata actgatcata atcagccata ccacatttgt agaggtttta cttgctttaa
     5821 aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta
     5881 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     5941 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6001 aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc
     6061 agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag
     6121 accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg
     6181 gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca
     6241 tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa
     6301 gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg
     6361 aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta
     6421 accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcaggtgg cacttttcgg
     6481 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     6541 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtcctgag
     6601 gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
     6661 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt
     6721 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     6781 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc
     6841 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg
     6901 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaagatcgat
     6961 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
     7021 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
     7081 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
     7141 gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag cgcggctatc gtggctggcc
     7201 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
     7261 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
     7321 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
     7381 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     7441 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     7501 gccaggctca aggcgagcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     7561 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     7621 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     7681 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     7741 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     7801 aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc accgccgcct
     7861 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7921 gcggggatct catgctggag ttcttcgccc accctagggg gaggctaact gaaacacgga
     7981 aggagacaat accggaagga acccgcgcta tgacggcaat aaaaagacag aataaaacgc
     8041 acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg
     8101 ataccccacc gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc
     8161 accccccaag ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaggccctg
     8221 ccatagcctc aggttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8281 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8341 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8401 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     8461 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     8521 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     8581 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     8641 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     8701 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     8761 tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     8821 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     8881 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     8941 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9001 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9061 attctgtgga taaccgtatt accgccatgc at
//
