LOCUS       V015546                 5264 bp    DNA     circular SYN 12-MAY-2020
DEFINITION  Exported.
ACCESSION   V015546
VERSION     V015546
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
   PUBMED   32341562
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV,
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI,
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA,
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV,
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 5264)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing
            ##Assembly-Data-END##
            SGRef: number: 1; type: "Journal Article"; journalName: "Nat.
            Biotechnol. (2020) In press"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways,
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry,
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
FEATURES             Location/Qualifiers
     source          1..5264
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             603..1868
                     /gene="h3h"
                     /label="Hispidin-3-hydroxylase"
                     /note="Hispidin-3-hydroxylase from Neonothopanus nambi.
                     Accession#: A0A3G9K5C8"
     misc_feature    1872..1928
                     /label="MCS"
                     /note="multiple cloning site"
     polyA_signal    2052..2173
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2180..2635)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2662..2766
                     /label="AmpR promoter"
     promoter        2768..3125
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3160..3951
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4186..4233
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4562..5150
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      601 ccatggcatc gtttgagaat tctctaagcg ttttgattgt cggggccgga cttggtgggc
      661 ttgctgctgc catcgcgctg cgtcgccaag ggcatgtcgt gaaaatatac gactcctcta
      721 gcttcaaagc cgaacttggt gcgggactcg ctgtgccgcc taacaccttg cgcagtctac
      781 agcaacttgg ttgcaatacc gagaacctca atggtgtgga taatctttgc ttcactgcga
      841 tggggtatga cgggagtgta gggatgatga acaacatgac tgactatcga gaggcatacg
      901 gtacttcttg gatcatggtc caccgcgttg acttgcataa cgagctgatg cgcgtagcac
      961 ttgatccagg tgggctcgga cctcctgcga cactccatct taatcatcgt gtcacattct
     1021 gcgatgtcga cgcttgcacc gtgacattca ccaacgggac cactcaatca gctgatctca
     1081 tcgttggtgc agacggtata cgctctacca ttcggcggtt tgtcttagaa gaagacgtga
     1141 ctgtgcctgc gtcaggaatc gtcgggtttc gatggcttgt acaagctgac gcgctggacc
     1201 catatcctga actcgactgg attgttaaaa agcctcctct aggcgcgcga ctgatctcca
     1261 ctcctcagaa tccacagtct ggtgttggct tggctgacag gcgcactatc atcatctacg
     1321 catgtcgtgg cggcaccatg gtcaatgtcc ttgcagtgca tgatgacgaa cgtgaccaga
     1381 acaccgcaga ttggagtgta ccggcttcca aagacgatct atttcgtgtt ttccacgatt
     1441 accatccacg ctttcggcgg cttttagagc ttgcgcagga tattaatctc tggcaaatgc
     1501 gtgttgtacc tgttttgaaa aaatgggtta acaagcgggt ttgcttgtta ggagatgctg
     1561 cgcacgcttc tttaccgacg ttgggtcaag gttttggtat gggtctggaa gatgccgtag
     1621 cacttggtac actccttcca aagggtacca ctgcatctca gatcgagact cgacttgcgg
     1681 tgtacgaaca gctacgtaag gatcgtgcgg aatttgttgc ggctgaatca tatgaagagc
     1741 aatatgttcc tgaaatgcgg ggactttatc tgaggtcaaa ggaactgcgt gatagagtca
     1801 tgggttatga tatcaaagtg gagagcgaga aggttctcga gacgctccta agaagttcta
     1861 attctgcctg aagatctcga gctcaagctt cgaattctgc agtcgacggt accgcgggcc
     1921 cgggatccac cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     1981 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     2041 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     2101 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     2161 tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     2221 ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     2281 tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     2341 ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     2401 ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     2461 cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     2521 agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     2581 acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     2641 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     2701 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     2761 aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     2821 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     2881 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2941 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     3001 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     3061 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     3121 gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     3181 cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     3241 acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     3301 tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     3361 tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     3421 ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     3481 gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     3541 ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     3601 atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     3661 gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     3721 catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     3781 gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     3841 attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     3901 gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     3961 ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     4021 ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     4081 tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     4141 ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     4201 agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     4261 ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     4321 tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     4381 cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     4441 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     4501 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     4561 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     4621 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     4681 tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     4741 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     4801 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     4861 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     4921 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     4981 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5041 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5101 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5161 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5221 cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//
