LOCUS       62056_18160        4916 bp DNA     circular SYN 12-MAY-2020
DEFINITION  Cloning vector pnnCPH-C1, complete sequence.
ACCESSION   MT233535
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4916)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Plants with genetically encoded autoluminescence
  JOURNAL   Nat. Biotechnol. (2020) In press
  PUBMED    32341562
REFERENCE   2  (bases 1 to 4916)
  AUTHORS   Mitiouchkina T, Mishin AS, Somermeyer LG, Markina NM, Chepurnyh TV, 
            Guglya EB, Karataeva TA, Palkina KA, Shakhova ES, Fakhranurova LI, 
            Chekova SV, Tsarkova AS, Golubev YV, Negrebetsky VV, Dolgushin SA, 
            Shalaev PV, Shlykov D, Melnik OA, Shipunova VO, Deyev SM, Bubyrev 
            AI, Pushin AS, Choob VV, Dolgov SV, Kondrashov FA, Yampolsky IV, 
            Sarkisyan KS.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-MAR-2020) Laboratory of Chemistry of Metabolic 
            Pathways, Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia
REFERENCE   3  (bases 1 to 4916)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Biotechnol. (2020) In press"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (23-MAR-2020) Laboratory of Chemistry of Metabolic Pathways, 
            Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, 
            Miklukho-Maklaya, 16/10, Moscow 117437, Russia"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..4916
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     gene            603..1523
                     /gene="cph"
                     /label=cph
     CDS             603..1523
                     /codon_start=1
                     /transl_table=11
                     /gene="cph"
                     /product="CPH"
                     /label=cph
                     /note="derived from N. nambi"
                     /protein_id="QJQ48093.1"
                     /translation="MAPISSTWSRLIRFVAVETSLVHIGEPIDATMDVGLARREGKTIQ
                     AYEIIGSGSALDLSAQVSKNVLTVRELLMPLSREEIKTVRCLGLNYPVHATEANVAVPK
                     FPNLFYKPVTSLIGPGGLITIPSVVQPPKEHQSDYEAELVIVIGKAAKNVSEDEALDYV
                     LGYTAANDISFRKHQLAVSQWSFSKGFDGTNPLGPCLVSASSIPDPQDIPIQCKLNGGV
                     VQNGNTRDQIFNVKKTISFLSQGTTLEPGSIILTGTPDGVGFVRNPPLYLKDGDEVMTW
                     IGSGIGTLANTVREEQTCFASGGHE"
     misc_feature    1524..1580
                     /label=MCS
                     /note="multiple cloning site"
     polyA_signal    1704..1825
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1832..2287)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2314..2418
                     /label=AmpR promoter
     promoter        2420..2777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2812..3603
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    3838..3885
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4214..4802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcaccg
      601 ccatggctcc catctctagt acgtggtcgc gtctgatccg tttcgtggcc gttgaaacat
      661 cattggtcca tatcggtgaa ccgattgatg cgacaatgga cgtaggcctg gcgcgccgtg
      721 aggggaaaac tatccaggcg tacgaaatca ttggatcagg gtcggccttg gacctgagtg
      781 cccaagtttc aaaaaatgtt ttgacagtcc gcgaattatt gatgccattg tcccgcgagg
      841 aaatcaagac ggtgcgttgc cttggattga actatcccgt tcatgccact gaagccaatg
      901 tagccgtacc caagttcccg aatcttttct acaagcctgt tacgtcactt attggtccag
      961 gtggtctgat taccatccct tccgttgtcc agcctcctaa ggaacatcag tctgactatg
     1021 aagccgaact ggtaatcgta atcggcaagg ccgccaaaaa tgtttcagag gacgaggcgt
     1081 tggattacgt gttaggttat acagccgcca acgatatctc gtttcgtaaa catcagttag
     1141 ctgtctccca atggagtttt tctaaaggat tcgacggtac gaatcctctt gggccatgcc
     1201 ttgtatcagc tagttcgatc cccgacccac aggatattcc tattcaatgc aagcttaatg
     1261 gtggtgtagt ccaaaatggg aacactcgtg accagatttt taatgtgaaa aaaaccatca
     1321 gttttctgtc acaaggaacg acattagagc cgggctccat cattttaacc ggaacgcccg
     1381 atggggtggg attcgtccgt aacccaccct tgtatcttaa agatggggat gaagtgatga
     1441 cctggatcgg ctccgggatt ggaacactgg cgaatacagt gcgcgaggaa caaacttgtt
     1501 tcgcctcggg tgggcatgaa taaagatctc gagctcaagc ttcgaattct gcagtcgacg
     1561 gtaccgcggg cccgggatcc accggatcta gataactgat cataatcagc cataccacat
     1621 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     1681 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     1741 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     1801 tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat tttgttaaaa
     1861 ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga aatcggcaaa
     1921 atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc agtttggaac
     1981 aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac cgtctatcag
     2041 ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc gaggtgccgt
     2101 aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg gggaaagccg
     2161 gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag ggcgctggca
     2221 agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc gccgctacag
     2281 ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2341 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2401 tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg tgtcagttag
     2461 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2701 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2761 gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg catgattgaa
     2821 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
     2881 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
     2941 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag
     3001 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
     3061 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
     3121 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
     3181 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
     3241 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
     3301 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat
     3361 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
     3421 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
     3481 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
     3541 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
     3601 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
     3661 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
     3721 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccta
     3781 gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc gctatgacgg
     3841 caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa cgcggggttc
     3901 ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc caatacgccc
     3961 gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca gggctcgcag
     4021 ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata ctttagattg
     4081 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4141 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4201 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     4261 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     4321 aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt
     4381 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     4441 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     4501 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     4561 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     4621 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     4681 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     4741 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     4801 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     4861 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc atgcat
//
