LOCUS       40924_32928       10135 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Tri-shuttle cloning vector pmycYACTn, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Benders GA, Noskov VN, Denisova EA, Lartigue C, Gibson DG, 
            Assad-Garcia N, Chuang RY, Carrera W, Moodie M, Algire MA, Phan Q, 
            Alperovich N, Vashee S, Merryman C, Venter JC, Smith HO, Glass JI, 
            Hutchison CA III.
  TITLE     Cloning whole bacterial genomes in yeast
  JOURNAL   Nucleic Acids Res. 38 (8), 2558-2569 (2010)
  PUBMED    20211840
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Benders GA, Gibson DG, Lartigue C, Glass JI.
  TITLE     Direct Submission
  JOURNAL   Submitted (02-FEB-2010) Synthetic Biology, J. Craig Venter 
            Institute, 10355 Science Center Dr., San Diego, CA 92121, USA
REFERENCE   3  (bases 1 to 10135)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10135)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2010"; volume: "38"; issue: "8"; pages: 
            "2558-2569"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (02-FEB-2010) Synthetic Biology, J. Craig Venter Institute, 10355 
            Science Center Dr., San Diego, CA 92121, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10135
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             84..1256
                     /codon_start=1
                     /product="IS256 transposase"
                     /label=IS256 transposase
                     /protein_id="ADE19325.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTEY
                     IQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKALM
                     ASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKNYPY
                     LMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEYLKER
                     GLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFREAVKGI
                     FKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRLKSTNLI
                     ERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
     promoter        1256..1326
                     /label=AmpR promoter
     CDS             1327..2184
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      2358..2946
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     mobile_element  3103..3128
                     /mobile_element_type="insertion sequence:IS256"
                     /label=insertion sequence:IS256
                     /note="outer inverted repeat"
     CDS             complement(3138..6182)
                     /label=lacZ
                     /note="beta-galactosidase"
     regulatory      complement(6219..6463)
                     /label=spiralin promoter
                     /note="spiralin promoter"
                     /regulatory_class="promoter"
     gene            complement(6464..8428)
                     /gene="tetM"
                     /label=tetM
     CDS             complement(6494..8428)
                     /codon_start=1
                     /gene="tetM"
                     /product="tetM"
                     /label=tetM
                     /protein_id="ADE19326.1"
                     /translation="MEENHMKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGT
                     TRTDNTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLI
                     SAKDGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKV
                     ELYPNVCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLY
                     HGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAYIRLYSGVLH
                     LRDSVRVSEKEKIKVTEMYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQ
                     RKKIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKV
                     QMEVISALLQEKYHVEIEITEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVST
                     PADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIVDTQLK
                     NNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRPNSRIDK
                     VRYMFNKIT"
     regulatory      complement(8435..8757)
                     /label=spiralin promoter
                     /note="spiralin promoter"
                     /regulatory_class="promoter"
     misc_feature    8758..9261
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an
                     autonomously replicating sequence"
     promoter        9262..9449
                     /label=HIS3 promoter
     CDS             9450..10106
                     /label=HIS3
                     /note="imidazoleglycerol-phosphate dehydratase, required
                     for histidine biosynthesis"
     mobile_element  complement(10110..10135)
                     /mobile_element_type="insertion sequence:IS256"
                     /label=insertion sequence:IS256
                     /note="inner inverted repeat"
ORIGIN
        1 gtgtaaaagt aaaaaggcca tataacagtc cttttacggt acaatgtttt taacgacaaa
       61 aacataccca ggaggacttt tacatgaccc aagtacattt tacactgaaa agcgaagaga
      121 ttcaaagcat tattgaatat tctgtaaagg atgacgtttc taaaaatatt ttaacaacgg
      181 tatttaatca actaatggaa aatcaacgaa cagaatatat tcaagcaaaa gaatatgaac
      241 gaacagaaaa ccgacaaagt caacgaaatg gctattatga gcgcagcttt acgacacgtg
      301 taggcacgct agaattaaaa gtacccagaa cacgtgatgg ccatttttca cccacagtgt
      361 ttgaacgtta tcaacgaaac gaaaaagccc tcatggcttc aatgttggaa atgtatgtat
      421 caggcgtttc aactcgtaaa gtatcaaaaa ttgtggaaga actttgtggt aaatccgtct
      481 ctaagtcctt cgtttctagc ttaacagaac agctagaacc tatggttaac gagtggcaga
      541 atcgtttatt atcagaaaaa aattatcctt acttaatgac cgatgtactc tatataaaag
      601 tacgagaaga aaatcgagta ctctcaaaaa gctgtcatat agcgattgga ataaccaaag
      661 atggcgaccg tgaaattatc ggcttcatga ttcaaagtgg cgaaagcgaa gagacctgga
      721 caacattttt tgaataccta aaagaacgcg gtttacaagg tacggaactc gttatttctg
      781 atgcgcacaa aggattagtc tctgccatta gaaaatcctt caccaacgta agttggcaaa
      841 gatgccaagt tcacttccta agaaatatct ttaccaccat tcctaaaaaa aattcaaaat
      901 ctttcagaga agctgttaaa ggaattttta agttcacaga tattaactta gcgcgtgagg
      961 ctaaaaatcg attgattcat gattatatcg atcaaccaaa atattcaaaa gcttgcgcat
     1021 cattggatga tggattcgaa gacgcctttc aatataccgt acaaggaaat tcccacaatc
     1081 gactaaagag taccaatcta attgaacgac tgaatcaaga agtacgcaga agagaaaaga
     1141 ttattcgcat cttccccaat caaacatcag ccaatcgctt aattggagcc gttcttatgg
     1201 acctacatga tgaatggatt tattcttcaa gaaaatacat caattttgat aagtagttca
     1261 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     1321 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     1381 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     1441 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     1501 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     1561 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     1621 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     1681 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     1741 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     1801 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     1861 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     1921 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     1981 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     2041 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     2101 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     2161 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     2221 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     2281 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     2341 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     2401 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     2461 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     2521 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2581 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2641 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2701 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     2761 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2821 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2881 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2941 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     3001 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     3061 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgataaagt ccgtataatt
     3121 gtgtaaaatt attattattt ttgacaccag accaactggt aatggtagcg accggcgctc
     3181 agctgtaatt ccgccgatac tgacgggctc caggagtcgt cgccaccaat ccccatatgg
     3241 aaaccgtcga tattcagcca tgtgccttct tccgcgtgca gcagatggcg atggctggtt
     3301 tccatcagtt gctgttgact gtagcggctg atgttgaact ggaagtcgcc gcgccactgg
     3361 tgtgggccat aattcaattc gcgcgtcccg cagcgcagac cgttttcgct cgggaagacg
     3421 tacggggtat acatgtctga caatggcaga tcccagcggt caaaacaggc ggcagtaagg
     3481 cggtcgggat agttttcttg cggccctaat ccgagccagt ttacccgctc tgctacctgc
     3541 gccagctggc agttcaggcc aatccgcgcc ggatgcggtg tatcgctcgc cacttcaaca
     3601 tcaacggtaa tcgccatttg accactacca tcaatccggt aggttttccg gctgataaat
     3661 aaggttttcc cctgatgctg ccacgcgtga gcggtcgtaa tcagcaccgc atcagcaagt
     3721 gtatctgccg tgcactgcaa caacgctgct tcggcctggt aatggcccgc cgccttccag
     3781 cgttcgaccc aggcgttagg gtcaatgcgg gtcgcttcac ttacgccaat gtcgttatcc
     3841 agcggtgcac gggtgaactg atcgcgcagc ggcgtcagca gttgtttttt atcgccaatc
     3901 cacatctgtg aaagaaagcc tgactggcgg ttaaattgcc aacgcttatt acccagctcg
     3961 atgcaaaaat ccatttcgct ggtggtcaga tgcgggatgg cgtgggacgc ggcggggagc
     4021 gtcacactga ggttttccgc cagacgccac tgctgccagg cgctgatgtg cccggcttct
     4081 gaccatgcgg tcgcgttcgg ttgcactacg cgtactgtga gccagagttg cccggcgctc
     4141 tccggctgcg gtagttcagg cagttcaatc aactgtttac cttgtggagc gacatccaga
     4201 ggcacttcac cgcttgccag cggcttacca tccagcgcca ccatccagtg caggagctcg
     4261 ttatcgctat gacggaacag gtattcgctg gtcacttcga tggtttgccc ggataaacgg
     4321 aactggaaaa actgctgctg gtgttttgct tccgtcagcg ctggatgcgg cgtgcggtcg
     4381 gcaaagacca gaccgttcat acagaactgg cgatcgttcg gcgtatcgcc aaaatcaccg
     4441 ccgtaagccg accacgggtt gccgttttca tcatatttaa tcagcgactg atccacccag
     4501 tcccagacga agccgccctg taaacgggga tactgacgaa acgcctgcca gtatttagcg
     4561 aaaccgccaa gactgttacc catcgcgtgg gcgtattcgc aaaggatcag cgggcgcgtc
     4621 tctccaggta gcgaaagcca ttttttgatg gaccatttcg gcacagccgg gaagggctgg
     4681 tcttcatcca cgcgcgcgta catcgggcaa ataatatcgg tggccgtggt gtcggctccg
     4741 ccgccttcat actgcaccgg gcgggaagga tcgacagatt tgatccagcg atacagcgcg
     4801 tcgtgattag cgccgtggcc tgattcattc cccagcgacc agatgatcac actcgggtga
     4861 ttacgatcgc gctgcaccat tcgcgttacg cgttcgctca tcgccggtag ccagcgcgga
     4921 tcatcggtca gacgattcat tggcaccatg ccgtgggttt caatattggc ttcatccacc
     4981 acatacaggc cgtagcggtc gcacagcgtg taccacagcg gatggttcgg ataatgcgaa
     5041 cagcgcacgg cgttaaagtt gttctgcttc atcagcagga tatcctgcac catcgtctgc
     5101 tcatccatga cctgaccatg cagaggatga tgctcgtgac ggttaacgcc tcgaatcagc
     5161 aacggcttgc cgttcagcag cagcagacca ttttcaatcc gcacctcgcg gaaaccgaca
     5221 tcgcaggctt ctgcttcaat cagcgtgccg tcggcggtgt gcagttcaac caccgcacga
     5281 tagagattcg ggatttcggc gctccacagt ttcgggtttt cgacgttcag acgtagtgtg
     5341 acgcgatcgg cataaccacc acgctcatcg ataatttcac cgccgaaagg cgcggtgccg
     5401 ctggcgacct gcgtttcacc ctgccataaa gaaactgtta cccgtaggta gtcacgcaac
     5461 tcgccgcaca tctgaacttc agcctccagt acagcgcggc tgaaatcatc attaaagcga
     5521 gtggcaacat ggaaatcgct gatttgtgta gtcggtttat gcagcaacga gacgtcacgg
     5581 aaaatgccgc tcatccgcca catatcctga tcttccagat aactgccgtc actccaacgc
     5641 agcaccatca ccgcgaggcg gttttctccg gcgcgtaaaa atgcgctcag gtcaaattca
     5701 gacggcaaac gactgtcctg gccgtaaccg acccagcgcc cgttgcacca cagatgaaac
     5761 gccgagttaa cgccatcaaa aataattcgc gtctggcctt cctgtagcca gctttcatca
     5821 acattaaatg tgagcgagta acaacccgtc ggattctccg tgggaacaaa cggcggattg
     5881 accgtaatgg gataggttac gttggtgtag atgggcgcat cgtaaccgtg catctgccag
     5941 tttgagggga cgacgacagt atcggcctca ggaagatcgc actccagcca gctttccggc
     6001 accgcttctg gtgccggaaa ccaggcaaag cgccattcgc cattcaggct gcgcaactgt
     6061 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
     6121 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
     6181 acgggatcgc gcttgagcag ctccttgctg gtgtccatag atcttatatt tcctttctct
     6241 attaagtagt gtttttatta aaagcaaatg taattaaaat tacattgaat atataagaga
     6301 aaaataacat taataataat tattaattgc ttctaaccct actttataca atttatatct
     6361 ttcttttttt ctttccctta tagtagcaat tgtacataat tttttaaaaa aaacaataaa
     6421 tatttcttaa aaaaacaaaa aaagattttc caaataaatt gcgtcagatc tttatataac
     6481 aacttaaatt acactaagtt attttattga acatatatcg tactttatct atccgactat
     6541 ttggacgacg gggctggcaa acaggttcac cggtagtaac atggtaccct tttaactctg
     6601 ttaaacaaac actacgtcca tttgtaaaga aagttaaatc actacgatat tcttgaatac
     6661 accgagcagg gatttctcca ctaagaatga cctcattatt tttcaattga gtgtctacga
     6721 tgttcgcaca atatttagga gcatcgttgt atgctcgtga aagatattcc tgtggcgcat
     6781 aaattttaaa actaagatat ggctctaaca attctgttcc agcttttttt aagacttgtt
     6841 ccaatacaat aggagcaagc atccgaaaat ctgctggggt actaacaggg ctatagtata
     6901 agccatactt aaaacagatt ttacagtccg tcacattcca accatacaat ccttgttcac
     6961 aaccatagcg tatcccttcc ataactgcat tttgaaatga ttgatttaag tatccaagag
     7021 aaaccgagct ctcatactgc attccacttc ccaacggaag cggtgataca gatagaccaa
     7081 tggaagccca gaaaggattt ggcggcactt cgatgtgaat ggtatattct gcatttttta
     7141 acggtctctc catataaatg actgtaggct ctgttatttc tatctccaca tgatactttt
     7201 cttgcaacag tgcactaatc acttccattt gtactttccc taagaaagaa agtataattt
     7261 catgtgtcgt agaatccacg taatatcgta gaagcggatc actatctgag atttccaaaa
     7321 gggcatcaag caacatttct ctctgttcag gtttactcgg ttcaacagtt gtttgtagta
     7381 gagggtgcgg attttcaatc ttttttctct gtggcaatag ttttgtatct ccaagaacac
     7441 tatttaactt caaaaactca ttttgcaaaa taacaatttc tccagaataa gctctatcaa
     7501 tcttacataa ttcaccattt attgaagtat acatttctgt aacttttatt ttttcctttt
     7561 ctgatactct aaccgaatct cgtaaatgta gtactccact ataaaggcgt atatatgcaa
     7621 gacgttgtct tttttttgta tattcaattt tgaaaacatt tccgcaaagt tcagacggac
     7681 ctcgatgtgt tgatgaataa aatttattag taataacttc tataaggtta tcaatcccta
     7741 tattactttt tgcacttcca tgataaagag ggaacagaga acaattctga aatcttatgc
     7801 tttcctcttg ttcgagttcc aatgcttcta atgatttacc ggacatatat ttctctaaaa
     7861 ggtcatcgtt tccctctatt accgtatccc attgttcaga ttcggtaaag ttcgtcacac
     7921 acacattagg atacagttct accttctgtt tgattacaat ttcggcagaa agtttctctt
     7981 taatatcctg ataaaccgtt gataaatcaa ttccattttg gtcaatctta ttgataaaaa
     8041 agattgtggg aatccccatt ttcctaagtg catgaaataa tatacgagtt tgtgcttgta
     8101 cgccatcttt tgcagaaatc agtagaattg ccccatctaa aactgataat gaacgatata
     8161 cttctgctaa gaaatccata tgtcctggcg tgtctatgat gttcaccttc gtattttccc
     8221 actgaaaaga ggttattcct gtctgaattg taattcctct ctgacgttct aaaagcgtat
     8281 tatccgtcct cgttgtacct ttgtccacgc ttcctaattc tgtaatcgct ccactgttat
     8341 ataataagct ttctgttaag gtagtttttc ctgcatcaac atgagctaaa actccaatat
     8401 taataatttt catgtgattt tcctccattc aaaggatctt atatttcctt tctctattaa
     8461 gtagtgtttt tattaaaagc aaatgtaatt aaaattacat tgaatatata agagaaaaat
     8521 aacattaata ataattatta attgcttcta accctacttt atacaattta tatctttctt
     8581 tttttctttc ccttatagta gcaattgtac ataatttttt aaaaaaaaca ataaatattt
     8641 cttaaaaaaa caaaaaaaga ttttccaaat aaattgctta atcttttttt gttttttcgt
     8701 cttcttttgg ttcagaaact ggtgcttcac tgttttcttg ttcactaact tttaattatc
     8761 acgtgctata aaaataatta taatttaaat tttttaatat aaatatataa attaaaaata
     8821 gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt ttccgaagat gtaaaagact
     8881 ctagggggat cgccaacaaa tactaccttt tatcttgctc ttcctgctct caggtattaa
     8941 tgccgaattg tttcatcttg tctgtgtaga agaccacaca cgaaaatcct gtgattttac
     9001 attttactta tcgttaatcg aatgtatatc tatttaatct gcttttcttg tctaataaat
     9061 atatatgtaa agtacgcttt ttgttgaaat tttttaaacc tttgtttatt tttttttctt
     9121 cattccgtaa ctcttctacc ttctttattt actttctaaa atccaaatac aaaacataaa
     9181 aataaataaa cacagagtaa attcccaaat tattccatca ttaaaagata cgaggcgcgt
     9241 gtaagttaca ggcaagcgat cctagtacac tctatatttt tttatgcctc ggtaatgatt
     9301 ttcatttttt tttttccacc tagcggatga ctcttttttt ttcttagcga ttggcattat
     9361 cacataatga attatacatt atataaagta atgtgatttc ttcgaagaat atactaaaaa
     9421 atgagcaggc aagataaacg aaggcaaaga tgacagagca gaaagcccta gtaaagcgta
     9481 ttacaaatga aaccaagatt cagattgcga tctctttaaa gggtggtccc ctagcgatag
     9541 agcactcgat cttcccagaa aaagaggcag aagcagtagc agaacaggcc acacaatcgc
     9601 aagtgattaa cgtccacaca ggtatagggt ttctggacca tatgatacat gctctggcca
     9661 agcattccgg ctggtcgcta atcgttgagt gcattggtga cttacacata gacgaccatc
     9721 acaccactga agactgcggg attgctctcg gtcaagcttt taaagaggcc ctactggcgc
     9781 gtggagtaaa aaggtttgga tcaggatttg cgcctttgga tgaggcactt tccagagcgg
     9841 tggtagatct ttcgaacagg ccgtacgcag ttgtcgaact tggtttgcaa agggagaaag
     9901 taggagatct ctcttgcgag atgatcccgc attttcttga aagctttgca gaggctagca
     9961 gaattaccct ccacgttgat tgtctgcgag gcaagaatga tcatcaccgt agtgagagtg
    10021 cgttcaaggc tcttgcggtt gccataagag aagccacctc gcccaatggt accaacgatg
    10081 ttccctccac caaaggtgtt cttatgtagt tttacacagg agtctggact tgact
//
