LOCUS       62056_16830        5139 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     primer_bind     571..587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          733..922
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             938..1870
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     promoter        1990..2020
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2074..2730
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      2825..3077
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3105..3121)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3131..3155
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3246..3834)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4008..4820)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      5111..5139
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt atcagcttgg caggaaacag ctatgaccat gattacgcca
      601 agcttgggcc gctcgagccg cccgggcgac ttcgcaagac ccttcctcta tataaggaag
      661 ttcatttcat ttggagagtc cggatccatt aacctgcagg taaatttcta gagctctggt
      721 agaaatctga gggtaaattt ctagtttttc tccttcattt tcttggttag gacccttttc
      781 tctttttatt tttttgagct ttgatctttc tttaaactga tctatttttt aattgattgg
      841 ttatggtgta aatattacat agctttaact gataatctga ttactttatt tcgtgtgtct
      901 atgatgatga tgatagttac agaaccgacg actcgtcatg acttcgaaag tttatgatcc
      961 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa
     1021 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat
     1081 ttttttacat ggtaacgcgg cctcttctta tttatggcga catgttgtgc cacatattga
     1141 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg
     1201 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct
     1261 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca
     1321 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga
     1381 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc
     1441 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc
     1501 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga
     1561 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg
     1621 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga
     1681 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga
     1741 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca
     1801 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa
     1861 aaatgaacaa taatctagcg catgcaccgg gccctggtag aaatctgagg aaccgacgac
     1921 tcgcttgttg aattagattg atctagagca tgcaccggtg atatcgcggc cgcattaggc
     1981 accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt gagttaggat
     2041 ccgtcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
     2101 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
     2161 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
     2221 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
     2281 catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
     2341 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
     2401 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
     2461 aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc agccaatccc
     2521 tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt cttcgccccc
     2581 gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc gctggcgatt
     2641 caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa tgaattacaa
     2701 cagtactgcg atgagtggca gggcggggcg taaacatgtc aggctccctt atacacagcc
     2761 agtctgcagg atatcgtcga cgaattcggc cggccgcttc gagcagacat gatacgcgtc
     2821 cagggatcgt tcaaacattt ggcaataaag tttcttaaga ttgaatcctg ttgccggtct
     2881 tgcgatgatt atcatataat ttctgttgaa ttacgttaag catgtaataa ttaacatgta
     2941 atgcatgacg ttatttatga gatgggtttt tatgattaga gtcccgcaat tatacattta
     3001 atacgcgata gaaaacaaaa tatagcgcgc aaactaggat aaattatcgc gcgcggtgtc
     3061 atctatgtta ctagatcggg ccaccaagat cttccccgga attcactggc cgtcgtttta
     3121 ccactagcct tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga
     3181 gatctcatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     3241 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     3301 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     3361 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     3421 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     3481 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     3541 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     3601 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     3661 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     3721 gttaccttcg gaagaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     3781 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     3841 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3901 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3961 tttaaatcaa tctaaagtat atatgtgtaa cattggtcta gtgattagaa aaactcatcg
     4021 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa
     4081 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc
     4141 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg
     4201 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat
     4261 ggcaaaagtt tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca
     4321 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga
     4381 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg
     4441 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg
     4501 aatgctgttt tccctgggat cgcagtggtg agtaaccatg catcatcagg agtacggata
     4561 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca
     4621 tctgtaacaa cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg
     4681 ggcttcccat acaatcggta gattgtcgca cctgattgcc cgacattatc gcgagcccat
     4741 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggccttga gcaagacgtt
     4801 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt
     4861 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca
     4921 acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat cacgcatctt
     4981 cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa ctggtccacc
     5041 tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga tggggcgatt
     5101 caggcgatcc ccatccaaca gcccgccgtc gagcgggct
//
