LOCUS       62056_16825       10923 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10923)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..10923
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        41..717
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a
                     duplicated enhancer region"
     CDS             785..1807
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    1850..2024
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    complement(2102..2126)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             2551..3342
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      3432..4020
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(4206..4346)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(4690..4884)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(4953..6023)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(6455..7081)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     misc_feature    complement(8381..8405)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     terminator      complement(8427..8679)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(8711..8728)
                     /codon_start=1
                     /label=6xHis
                     /note="6xHis affinity tag"
                     /translation="HHHHHH"
     CDS             complement(8735..10558)
                     /codon_start=1
                     /label=GUS
                     /note="beta-glucuronidase"
                     /translation="LQNRRLVRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQ
                     ESRAIAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWV
                     NNQEVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQ
                     SYFHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELR
                     DADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYAE
                     EMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAIKE
                     LIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCDAHT
                     DTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVDTLAG
                     LHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGNKKGIF
                     TRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     intron          complement(10553..10742)
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     promoter        complement(10787..10833)
                     /label=minimal CaMV 35S promoter
                     /note="minimal 35S promoter from cauliflower mosaic virus"
ORIGIN
        1 agaatatcaa agatacagtc tcagaagacc aaagggctat tgagactttt caacaaaggg
       61 taatatcggg aaacctcctc ggattccatt gcccagctat ctgtcacttc atcaaaagga
      121 cagtagaaaa ggaaggtggc acctacaaat gccatcattg cgataaagga aaggctatcg
      181 ttcaagatgc ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg
      241 tggaaaaaga agacgttcca accacgtctt caaagcaagt ggattgatgt gaacatggtg
      301 gagcacgaca ctctcgtcta ctccaagaat atcaaagata cagtctcaga agaccaaagg
      361 gctattgaga cttttcaaca aagggtaata tcgggaaacc tcctcggatt ccattgccca
      421 gctatctgtc acttcatcaa aaggacagta gaaaaggaag gtggcaccta caaatgccat
      481 cattgcgata aaggaaaggc tatcgttcaa gatgcctctg ccgacagtgg tcccaaagat
      541 ggacccccac ccacgaggag catcgtggaa aaagaagacg ttccaaccac gtcttcaaag
      601 caagtggatt gatgtgatat ctccactgac gtaagggatg acgcacaatc ccactatcct
      661 tcgcaagacc cttcctctat ataaggaagt tcatttcatt tggagaggac acgctgaaat
      721 caccagtctc tctctacaaa tctatctctc tcgagctttc gcagatcccg gggggcaatg
      781 agatatgaaa aagcctgaac tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt
      841 cgacagcgtc tccgacctga tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt
      901 cgatgtagga gggcgtggat atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa
      961 agatcgttat gtttatcggc actttgcatc ggccgcgctc ccgattccgg aagtgcttga
     1021 cattggggag tttagcgaga gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac
     1081 gttgcaagac ctgcctgaaa ccgaactgcc cgctgttcta caaccggtcg cggaggctat
     1141 ggatgcgatc gctgcggccg atcttagcca gacgagcggg ttcggcccat tcggaccgca
     1201 aggaatcggt caatacacta catggcgtga tttcatatgc gcgattgctg atccccatgt
     1261 gtatcactgg caaactgtga tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga
     1321 tgagctgatg ctttgggccg aggactgccc cgaagtccgg cacctcgtgc acgcggattt
     1381 cggctccaac aatgtcctga cggacaatgg ccgcataaca gcggtcattg actggagcga
     1441 ggcgatgttc ggggattccc aatacgaggt cgccaacatc ttcttctgga ggccgtggtt
     1501 ggcttgtatg gagcagcaga cgcgctactt cgagcggagg catccggagc ttgcaggatc
     1561 gccacgactc cgggcgtata tgctccgcat tggtcttgac caactctatc agagcttggt
     1621 tgacggcaat ttcgatgatg cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc
     1681 cggagccggg actgtcgggc gtacacaaat cgcccgcaga agcgcggccg tctggaccga
     1741 tggctgtgta gaagtactcg ccgatagtgg aaaccgacgc cccagcactc gtccgagggc
     1801 aaagaaatag agtagatgcc gaccggatct gtcgatcgac aagctcgagt ttctccataa
     1861 taatgtgtga gtagttccca gataagggaa ttagggttcc tatagggttt cgctcatgtg
     1921 ttgagcatat aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa
     1981 atttctaatt cctaaaacca aaatccagta ctaaaatcca gatcccccga attaattcgg
     2041 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     2101 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     2161 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     2221 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     2281 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     2341 acagagcgtt gctgcctgtg atcaccgcgg tttcaaaatc ggctccgtcg atactatgtt
     2401 atacgccaac tttgaaaaca actttgaaaa agctgttttc tggtatttaa ggttttagaa
     2461 tgcaaggaac agtgaattgg agttcgtctt gttataatta gcttcttggg gtatctttaa
     2521 atactgtaga aaagaggaag gaaataataa atggctaaaa tgagaatatc accggaattg
     2581 aaaaaactga tcgaaaaata ccgctgcgta aaagatacgg aaggaatgtc tcctgctaag
     2641 gtatataagc tggtgggaga aaatgaaaac ctatatttaa aaatgacgga cagccggtat
     2701 aaagggacca cctatgatgt ggaacgggaa aaggacatga tgctatggct ggaaggaaag
     2761 ctgcctgttc caaaggtcct gcactttgaa cggcatgatg gctggagcaa tctgctcatg
     2821 agtgaggccg atggcgtcct ttgctcggaa gagtatgaag atgaacaaag ccctgaaaag
     2881 attatcgagc tgtatgcgga gtgcatcagg ctctttcact ccatcgacat atcggattgt
     2941 ccctatacga atagcttaga cagccgctta gccgaattgg attacttact gaataacgat
     3001 ctggccgatg tggattgcga aaactgggaa gaagacactc catttaaaga tccgcgcgag
     3061 ctgtatgatt ttttaaagac ggaaaagccc gaagaggaac ttgtcttttc ccacggcgac
     3121 ctgggagaca gcaacatctt tgtgaaagat ggcaaagtaa gtggctttat tgatcttggg
     3181 agaagcggca gggcggacaa gtggtatgac attgccttct gcgtccggtc gatcagggag
     3241 gatatcgggg aagaacagta tgtcgagcta ttttttgact tactggggat caagcctgat
     3301 tgggagaaaa taaaatatta tattttactg gatgaattgt tttagtacct agaatgcatg
     3361 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
     3421 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
     3481 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
     3541 gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
     3601 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
     3661 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
     3721 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
     3781 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
     3841 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
     3901 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
     3961 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
     4021 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
     4081 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
     4141 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
     4201 gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg
     4261 tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat
     4321 cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct
     4381 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     4441 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt gccttgatgt
     4501 gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag attgcctggc
     4561 cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga agcggcgggg
     4621 cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc tgtgcgctgg
     4681 ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt taaagagttt
     4741 taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg tgaccggttc
     4801 ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta cggctttggg
     4861 ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc ccctgctagg
     4921 gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc gccctcgatc
     4981 aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc cttcaaatcg
     5041 tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa cttcttgaac
     5101 tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc ttctgccttg
     5161 cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc gatcaaaaag
     5221 taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc gcggtacatc
     5281 caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt tacgatcttg
     5341 tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt cttggccttc
     5401 ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc taccaggtcg
     5461 tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc aacgtgtgga
     5521 cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat ggattcggtt
     5581 agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat gccggccggc
     5641 cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc gccagctcgt
     5701 cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact atcgcgggtg
     5761 cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt gggcggcttc
     5821 ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat tcgatcagcg
     5881 gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg ctgggcggcc
     5941 tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat ttgtaccggg
     6001 ccggatggtt tgcgaccgct cacgccgatt cctcgggctt gggggttcca gtgccattgc
     6061 agggccggca gacaacccag ccgcttacgc ctggccaacc gcccgttcct ccacacatgg
     6121 ggcattccac ggcgtcggtg cctggttgtt cttgattttc catgccgcct cctttagccg
     6181 ctaaaattca tctactcatt tattcatttg ctcatttact ctggtagctg cgcgatgtat
     6241 tcagatagca gctcggtaat ggtcttgcct tggcgtaccg cgtacatctt cagcttggtg
     6301 tgatcctccg ccggcaactg aaagttgacc cgcttcatgg ctggcgtgtc tgccaggctg
     6361 gccaacgttg cagccttgct gctgcgtgcg ctcggacggc cggcacttag cgtgtttgtg
     6421 cttttgctca ttttctcttt acctcattaa ctcaaatgag ttttgattta atttcagcgg
     6481 ccagcgcctg gacctcgcgg gcagcgtcgc cctcgggttc tgattcaaga acggttgtgc
     6541 cggcggcggc agtgcctggg tagctcacgc gctgcgtgat acgggactca agaatgggca
     6601 gctcgtaccc ggccagcgcc tcggcaacct caccgccgat gcgcgtgcct ttgatcgccc
     6661 gcgacacgac aaaggccgct tgtagccttc catccgtgac ctcaatgcgc tgcttaacca
     6721 gctccaccag gtcggcggtg gcccatatgt cgtaagggct tggctgcacc ggaatcagca
     6781 cgaagtcggc tgccttgatc gcggacacag ccaagtccgc cgcctggggc gctccgtcga
     6841 tcactacgaa gtcgcgccgg ccgatggcct tcacgtcgcg gtcaatcgtc gggcggtcga
     6901 tgccgacaac ggttagcggt tgatcttccc gcacggccgc ccaatcgcgg gcactgccct
     6961 ggggatcgga atcgactaac agaacatcgg ccccggcgag ttgcagggcg cgggctagat
     7021 gggttgcgat ggtcgtcttg cctgacccgc ctttctggtt aagtacagcg ataaccttca
     7081 tgcgttcccc ttgcgtattt gtttatttac tcatcgcatc atatacgcag cgaccgcatg
     7141 acgcaagctg ttttactcaa atacacatca cctttttaga cggcggcgct cggtttcttc
     7201 agcggccaag ctggccggcc aggccgccag cttggcatca gacaaaccgg ccaggatttc
     7261 atgcagccgc acggttgaga cgtgcgcggg cggctcgaac acgtacccgg ccgcgatcat
     7321 ctccgcctcg atctcttcgg taatgaaaaa cggttcgtcc tggccgtcct ggtgcggttt
     7381 catgcttgtt cctcttggcg ttcattctcg gcggccgcca gggcgtcggc ctcggtcaat
     7441 gcgtcctcac ggaaggcacc gcgccgcctg gcctcggtgg gcgtcacttc ctcgctgcgc
     7501 tcaagtgcgc ggtacagggt cgagcgatgc acgccaagca gtgcagccgc ctctttcacg
     7561 gtgcggcctt cctggtcgat cagctcgcgg gcgtgcgcga tctgtgccgg ggtgagggta
     7621 gggcgggggc caaacttcac gcctcgggcc ttggcggcct cgcgcccgct ccgggtgcgg
     7681 tcgatgatta gggaacgctc gaactcggca atgccggcga acacggtcaa caccatgcgg
     7741 ccggccggcg tggtggtgtc ggcccacggc tctgccaggc tacgcaggcc cgcgccggcc
     7801 tcctggatgc gctcggcaat gtccagtagg tcgcgggtgc tgcgggccag gcggtctagc
     7861 ctggtcactg tcacaacgtc gccagggcgt aggtggtcaa gcatcctggc cagctccggg
     7921 cggtcgcgcc tggtgccggt gatcttctcg gaaaacagct tggtgcagcc ggccgcgtgc
     7981 agttcggccc gttggttggt caagtcctgg tcgtcggtgc tgacgcgggc atagcccagc
     8041 aggccagcgg cggcgctctt gttcatggcg taatgtctcc ggttctagtc gcaagtattc
     8101 tactttatgc gactaaaaca cgcgacaaga aaacgccagg aaaagggcag ggcggcagcc
     8161 tgtcgcgtaa cttaggactt gtgcgacatg tcgttttcag aagacggctg cactgaacgt
     8221 cagaagccga ctgcactata gcagcggagg ggttggatca aagtactttg atcccgaggg
     8281 gaaccctgtg gttggcatgc acatacaaat ggacgaacgg ataaaccttt tcacgccctt
     8341 ttaaatatcc gttattctaa taaacgctct tttctcttag gtttacccgc caatatatcc
     8401 tgtcaaacac tgatagttta attcccgatc tagtaacata gatgacaccg cgcgcgataa
     8461 tttatcctag tttgcgcgct atattttgtt ttctatcgcg tattaaatgt ataattgcgg
     8521 gactctaatc ataaaaaccc atctcataaa taacgtcatg cattacatgt taattattac
     8581 atgcttaacg taattcaaca gaaattatat gataatcatc gcaagaccgg caacaggatt
     8641 caatcttaag aaactttatt gccaaatgtt tgaacgatcg gggaaattcg agctggtcac
     8701 caattcacac gtggtggtgg tggtggtggc tagcttgttt gcctccctgc tgcggttttt
     8761 caccgaagtt catgccagtc cagcgttttt gcagcagaaa agccgccgac ttcggtttgc
     8821 ggtcgcgagt gaagatccct ttcttgttac cgccaacgcg caatatgcct tgcgaggtcg
     8881 caaaatcggc gaaattccat acctgttcac cgacgacggc gctgacgcga tcaaagacgc
     8941 ggtgatacat atccagccat gcacactgat actcttcact ccacatgtcg gtgtacattg
     9001 agtgcagccc ggctaacgta tccacgccgt attcggtgat gataatcggc tgatgcagtt
     9061 tctcctgcca ggccagaagt tctttttcca gtaccttctc tgccgtttcc aaatcgccgc
     9121 tttggacata ccatccgtaa taacggttca ggcacagcac atcaaagaga tcgctgatgg
     9181 tatcggtgtg agcgtcgcag aacattacat tgacgcaggt gatcggacgc gtcgggtcga
     9241 gtttacgcgt tgcttccgcc agtggcgcga aatattcccg tgcaccttgc ggacgggtat
     9301 ccggttcgtt ggcaatactc cacatcacca cgcttgggtg gtttttgtca cgcgctatca
     9361 gctctttaat cgcctgtaag tgcgcttgct gagtttcccc gttgactgcc tcttcgctgt
     9421 acagttcttt cggcttgttg cccgcttcga aaccaatgcc taaagagagg ttaaagccga
     9481 cagcagcagt ttcatcaatc accacgatgc catgttcatc tgcccagtcg agcatctctt
     9541 cagcgtaagg gtaatgcgag gtacggtagg agttggcccc aatccagtcc attaatgcgt
     9601 ggtcgtgcac catcagcacg ttatcgaatc ctttgccacg caagtccgca tcttcatgac
     9661 gaccaaagcc agtaaagtag aacggtttgt ggttaatcag gaactgttcg cccttcactg
     9721 ccactgaccg gatgccgacg cgaagcgggt agatatcaca ctctgtctgg cttttggctg
     9781 tgacgcacag ttcatagaga taaccttcac ccggttgcca gaggtgcgga ttcaccactt
     9841 gcaaagtccc gctagtgcct tgtccagttg caaccacctg ttgatccgca tcacgcagtt
     9901 caacgctgac atcaccattg gccaccacct gccagtcaac agacgcgtgg ttacagtctt
     9961 gcgcgacatg cgtcaccacg gtgatatcgt ccacccaggt gttcggcgtg gtgtagagca
    10021 ttacgctgcg atggattccg gcatagttaa agaaatcatg gaagtaagac tgctttttct
    10081 tgccgttttc gtcggtaatc accattcccg gcgggatagt ctgccagttc agttcgttgt
    10141 tcacacaaac ggtgatacgt acacttttcc cggcaataac atacggcgtg acatcggctt
    10201 caaatggcgt atagccgccc tgatgctcca tcacttcctg attattgacc cacactttgc
    10261 cgtaatgagt gaccgcatcg aaacgcagca cgatacgctg gcctgcccaa cctttcggta
    10321 taaagacttc gcgctgatac cagacgttgc ccgcataatt acgaatatct gcatcggcga
    10381 actgatcgtt aaaactgcct ggcacagcaa ttgcccggct ttcttgtaac gcgctttccc
    10441 accaacgctg atcaattcca cagttttcgc gatccagact gaatgcccac aggccgtcga
    10501 gttttttgat ttcacgggtt ggggtttcta caggacggac gagtcgtcgg ttctgtaact
    10561 atcatcatca tcatagacac acgaaataaa gtaatcagat tatcagttaa agctatgtaa
    10621 tatttacacc ataaccaatc aattaaaaaa tagatcagtt taaagaaaga tcaaagctca
    10681 aaaaaataaa aagagaaaag ggtcctaacc aagaaaatga aggagaaaaa ctagaaattt
    10741 accctcagat ctaccatgga ctagtgatgg aattcccggc agcgtgtcct ctccaaatga
    10801 aatgaacttc cttatataga ggaagggtct tgcgaaggat agtgggattg gatccatcga
    10861 cctgcagcca agcttggcgc gccgggccca acatggtgga gcacgacact ctcgtctact
    10921 cca
//
