LOCUS       62056_16820        5856 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5856
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     primer_bind     571..587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          733..922
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             938..2587
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     promoter        2707..2737
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             2791..3447
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     terminator      3542..3794
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3822..3838)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3848..3872
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3963..4551)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4725..5537)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      5828..5856
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt atcagcttgg caggaaacag ctatgaccat gattacgcca
      601 agcttgggcc gctcgagccg cccgggcgac ttcgcaagac ccttcctcta tataaggaag
      661 ttcatttcat ttggagagtc cggatccatt aacctgcagg taaatttcta gagctctggt
      721 agaaatctga gggtaaattt ctagtttttc tccttcattt tcttggttag gacccttttc
      781 tctttttatt tttttgagct ttgatctttc tttaaactga tctatttttt aattgattgg
      841 ttatggtgta aatattacat agctttaact gataatctga ttactttatt tcgtgtgtct
      901 atgatgatga tgatagttac agaaccgacg actcgtcatg gaagacgcca aaaacataaa
      961 gaaaggcccg gcgccattct atccgctgga agatggaacc gctggagagc aactgcataa
     1021 ggctatgaag agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga
     1081 ggtggacatc acttacgctg agtacttcga aatgtccgtt cggttggcag aagctatgaa
     1141 acgatatggg ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt
     1201 ctttatgccg gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat
     1261 ttataatgaa cgtgaattgc tcaacagtat gggcatttcg cagcctaccg tggtgttcgt
     1321 ttccaaaaag gggttgcaaa aaattttgaa cgtgcaaaaa aagctcccaa tcatccaaaa
     1381 aattattatc atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt
     1441 cacatctcat ctacctcccg gttttaatga atacgatttt gtgccagagt ccttcgatag
     1501 ggacaagaca attgcactga tcatgaactc ctctggatct actggtctgc ctaaaggtgt
     1561 cgctctgcct catagaactg cctgcgtgag attctcgcat gccagagatc ctatttttgg
     1621 caatcaaatc attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg
     1681 aatgtttact acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt
     1741 tgaagaagag ctgtttctga ggagccttca ggattacaag attcaaagtg cgctgctggt
     1801 gccaacccta ttctccttct tcgccaaaag cactctgatt gacaaatacg atttatctaa
     1861 tttacacgaa attgcttctg gtggcgctcc cctctctaag gaagtcgggg aagcggttgc
     1921 caagaggttc catctgccag gtatcaggca aggatatggg ctcactgaga ctacatcagc
     1981 tattctgatt acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt
     2041 ttttgaagcg aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcaaagagg
     2101 cgaactgtgt gtgagaggtc ctatgattat gtccggttat gtaaacaatc cggaagcgac
     2161 caacgccttg attgacaagg atggatggct acattctgga gacatagctt actgggacga
     2221 agacgaacac ttcttcatcg ttgaccgcct gaagtctctg attaagtaca aaggctatca
     2281 ggtggctccc gctgaattgg aatccatctt gctccaacac cccaacatct tcgacgcagg
     2341 tgtcgcaggt cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga
     2401 gcacggaaag acgatgacgg aaaaagagat cgtggattac gtcgccagtc aagtaacaac
     2461 cgcgaaaaag ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg
     2521 aaaactcgac gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagat
     2581 cgccgtgtaa tctagcgcat gcaccgggcc ctggtagaaa tctgaggaac cgacgactcg
     2641 cttgttgaat tagattgatc tagagcatgc accggtgata tcgcggccgc attaggcacc
     2701 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg
     2761 tcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc
     2821 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa
     2881 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa
     2941 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat
     3001 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct
     3061 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac
     3121 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac
     3181 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg
     3241 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt
     3301 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag
     3361 gttcatcatg ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag
     3421 tactgcgatg agtggcaggg cggggcgtaa acatgtcagg ctcccttata cacagccagt
     3481 ctgcaggata tcgtcgacga attcggccgg ccgcttcgag cagacatgat acgcgtccag
     3541 ggatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     3601 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     3661 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     3721 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     3781 tatgttacta gatcgggcca ccaagatctt ccccggaatt cactggccgt cgttttacca
     3841 ctagccttga caggatatat tggcgggtaa actaagtcgc tgtatgtgtt tgtttgagat
     3901 ctcatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg
     3961 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     4021 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     4081 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
     4141 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
     4201 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     4261 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
     4321 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
     4381 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
     4441 accttcggaa gaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
     4501 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
     4561 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
     4621 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
     4681 aaatcaatct aaagtatata tgtgtaacat tggtctagtg attagaaaaa ctcatcgagc
     4741 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     4801 cgtttctgta atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg
     4861 tatcggtctg cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca
     4921 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     4981 aaaagtttat gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     5041 aaatcactcg catcaaccaa accgttattc attcgtgatt gcgcctgagc gagacgaaat
     5101 acgcgatcgc tgttaaaagg acaattacaa acaggaatcg aatgcaaccg gcgcaggaac
     5161 actgccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaat
     5221 gctgttttcc ctgggatcgc agtggtgagt aaccatgcat catcaggagt acggataaaa
     5281 tgcttgatgg tcggaagagg cataaattcc gtcagccagt ttagtctgac catctcatct
     5341 gtaacaacat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     5401 ttcccataca atcggtagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     5461 tacccatata aatcagcatc catgttggaa tttaatcgcg gccttgagca agacgtttcc
     5521 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     5581 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     5641 tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac gcatcttccc
     5701 gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg gtccacctac
     5761 aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg ggcgattcag
     5821 gcgatcccca tccaacagcc cgccgtcgag cgggct
//
