LOCUS       62056_16815        5643 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5643)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5643
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA
                     (truncated)"
     primer_bind     571..587
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     intron          733..922
                     /label=cat1 intron
                     /note="castor bean catalase intron, modified"
     CDS             1019..1726
                     /codon_start=1
                     /label=mgfp5
                     /note="GFP with folding enhancement mutations"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLCYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKAN
                     FKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELY"
     promoter        1849..1879
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1933..2589
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2934..3236
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFRVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     terminator      3329..3581
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3609..3625)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    3635..3659
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3750..4338)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4512..5324)
                     /codon_start=1
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANVVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      5615..5643
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt atcagcttgg caggaaacag ctatgaccat gattacgcca
      601 agcttgggcc gctcgagccg cccgggcgac ttcgcaagac ccttcctcta tataaggaag
      661 ttcatttcat ttggagagtc cggatccatt aacctgcagg taaatttcta gagctctggt
      721 agaaatctga gggtaaattt ctagtttttc tccttcattt tcttggttag gacccttttc
      781 tctttttatt tttttgagct ttgatctttc tttaaactga tctatttttt aattgattgg
      841 ttatggtgta aatattacat agctttaact gataatctga ttactttatt tcgtgtgtct
      901 atgatgatga tgatagttac agaaccgacg actcgtcatg acatccactt tgcctttctc
      961 tccacaggtg tccactccca ggtccaactg caggtcgcct gcaggcttaa ccatggctag
     1021 caaaggagaa gaactcttca ctggagttgt cccaattctt gttgaattag atggtgatgt
     1081 aaacggccac aagttctctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1141 taccctgaag ttcatctgca ctactggcaa actgcctgtt ccctggccaa cactagtcac
     1201 tactctgtgc tatggtgttc aatgcttttc aagatacccg gatcatatga aacggcatga
     1261 ctttttcaag agtgccatgc ccgaaggtta tgtccaggaa aggaccatct tcttcaaaga
     1321 tgacggcaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1381 aatcgagtta aaaggtattg acttcaagga agatggcaac attctgggac acaaattgga
     1441 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1501 agcgaacttc aagacccgcc acaacattga agatggaagc gttcaactag cagaccatta
     1561 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1621 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1681 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctgtataact gatctagcgc
     1741 atgcaccggg ccctggtaga aatctgagga accgacgact cgcttgttga attagattga
     1801 tctagagcat gcaccggtga tatcgcggcc gcattaggca ccccaggctt tacactttat
     1861 gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt ttcaggagct
     1921 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1981 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     2041 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     2101 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     2161 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     2221 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     2281 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     2341 gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt caccagtttt
     2401 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     2461 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     2521 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     2581 ggcggggcgt aaacgcgtgg atccggctta ctaaaagcca gataacagta tgcgtatttg
     2641 cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag tatgtcaaaa
     2701 agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc tatcagttgc
     2761 tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc agaatgaagc
     2821 ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg ctgaggtcgc
     2881 ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt gaaatgcagt
     2941 ttagggttta cacctataaa agagagagcc gttatcgtct gtttgtggat gtacagagtg
     3001 atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca cgtctgctgt
     3061 cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa agctggcgca
     3121 tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa gtggctgatc
     3181 tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg ggaatataaa
     3241 tgtcaggctc ccttatacac agccagtctg caggatatcg tcgacgaatt cggccggccg
     3301 cttcgagcag acatgatacg cgtccaggga tcgttcaaac atttggcaat aaagtttctt
     3361 aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt tgaattacgt
     3421 taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg tttttatgat
     3481 tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc gcgcaaacta
     3541 ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggccacca agatcttccc
     3601 cggaattcac tggccgtcgt tttaccacta gccttgacag gatatattgg cgggtaaact
     3661 aagtcgctgt atgtgtttgt ttgagatctc atgtgagcaa aaggccagca aaaggccagg
     3721 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     3781 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     3841 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     3901 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     3961 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     4021 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     4081 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     4141 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     4201 ggtatctgcg ctctgctgaa gccagttacc ttcggaagaa gagttggtag ctcttgatcc
     4261 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     4321 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     4381 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     4441 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatgt gtaacattgg
     4501 tctagtgatt agaaaaactc atcgagcatc aaatgaaact gcaatttatt catatcagga
     4561 ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa ctcaccgagg
     4621 cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg tccaacatca
     4681 atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa atcaccatga
     4741 gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca gacttgttca
     4801 acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc gttattcatt
     4861 cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt taaaaggaca attacaaaca
     4921 ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt ttcacctgaa
     4981 tcaggatatt cttctaatac ctggaatgct gttttccctg ggatcgcagt ggtgagtaac
     5041 catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat aaattccgtc
     5101 agccagttta gtctgaccat ctcatctgta acaacattgg caacgctacc tttgccatgt
     5161 ttcagaaaca actctggcgc atcgggcttc ccatacaatc ggtagattgt cgcacctgat
     5221 tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat gttggaattt
     5281 aatcgcggcc ttgagcaaga cgtttcccgt tgaatatggc tcataacacc ccttgtatta
     5341 ctgtttatgt aagcagacag ttttattgtt catgatgata tatttttatc ttgtgcaatg
     5401 taacatcaga gattttgaga cacaacgtgg ctttgttgaa taaatcgaac ttttgctgag
     5461 ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc aaagcaaaag
     5521 ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg ctccctcact
     5581 ttctggctgg atgatggggc gattcaggcg atccccatcc aacagcccgc cgtcgagcgg
     5641 gct
//
