LOCUS       40924_31265        3338 bp DNA     circular SYN 01-JAN-1980
DEFINITION  Vector for expressing mOrange2 in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3338)
  AUTHORS   Clontech
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 3338)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        143..173
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             282..989
                     /codon_start=1
                     /label=mOrange2
                     /note="photostable monomeric orange derivative of DsRed 
                     fluorescent protein (Shaner et al., 2008)"
                     /translation="MVSKGEENNMAIIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEG
                     FQTAKLKVTKGGPLPFAWDILSPHFTYGSKAYVKHPADIPDYFKLSFPEGFKWERVMNY
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GKIKMRLKLKDGGHYTSEVKTTYKAKKPVQLPGAYIVDIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     promoter        1433..1537
                     /label=AmpR promoter
     CDS             1538..2395
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2569..3157
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
      241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tatggtgagc aagggcgagg
      301 agaataacat ggccatcatc aaggagttca tgcgcttcaa ggtgcgcatg gagggctccg
      361 tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggctttc
      421 agaccgctaa gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt
      481 cccctcattt cacctacggc tccaaggcct acgtgaagca ccccgccgac atccccgact
      541 acttcaagct gtccttcccc gagggcttca agtgggagcg cgtgatgaac tacgaggacg
      601 gcggcgtggt gaccgtgacc caggactcct ccctgcagga cggcgagttc atctacaagg
      661 tgaagctgcg cggcaccaac ttcccctccg acggccccgt gatgcagaag aagaccatgg
      721 gctgggaggc ctcctccgag cggatgtacc ccgaggacgg tgccctgaag ggcaagatca
      781 agatgaggct gaagctgaag gacggcggcc actacacctc cgaggtcaag accacctaca
      841 aggccaagaa gcccgtgcag ctgcccggcg cctacatcgt cgacatcaag ttggacatca
      901 cctcccacaa cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact
      961 ccaccggcgg catggacgag ctgtacaagt gagcggccgc gactctagaa ttccaactga
     1021 gcgccggtcg ctaccattac caacttgtct ggtgtcaaaa ataataggcc tactagtcgg
     1081 ccgtacgggc cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
     1141 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
     1201 cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag
     1261 cagattgtac tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga
     1321 aaataccgca tcaggcggcc ttaagggcct cgtgatacgc ctatttttat aggttaatgt
     1381 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     1441 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     1501 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     1561 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     1621 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     1681 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     1741 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     1801 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     1861 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     1921 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     1981 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     2041 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     2101 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     2161 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     2221 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     2281 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     2341 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     2401 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     2461 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     2521 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     2581 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     2641 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     2701 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     2761 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     2821 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     2881 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     2941 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3001 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3061 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3121 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3181 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3241 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     3301 gaccgagcgc agcgagtcag tgagcgagga agcggaag
//
