LOCUS       V015414                 7425 bp    DNA     circular SYN 01-AUG-2019
DEFINITION  Exported.
ACCESSION   V015414
VERSION     V015414
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Soleymani S, Kalidari GA, Bassami MR, Dehghani H, Hashemi Tabar GR.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUN-2019) Pathobiology, Division of Biotechnology,
            Faculty of Veterinary Medicine, Ferdowsi University of Mashhad,
            Azadi Square, Mashhad, Razavi Khorasan 9177948974, Iran
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Submitted
            (10-JUN-2019) Pathobiology, Division of Biotechnology, Faculty of
            Veterinary Medicine, Ferdowsi University of Mashhad, Azadi Square,
            Mashhad, Razavi Khorasan 9177948974, Iran"
FEATURES             Location/Qualifiers
     source          1..7425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    254..275
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     CDS             1050..1238
                     /label="rop"
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    1343..1485
                     /label="bom"
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(1671..2259)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     promoter        2347..2375
                     /label="Pc promoter"
                     /note="class 1 integron promoter"
     CDS             2564..3094
                     /label="GmR"
                     /note="gentamycin acetyltransferase"
     promoter        3387..3464
                     /label="lacI promoter"
     CDS             3465..4544
                     /label="lacI"
                     /note="lac repressor"
     promoter        4698..4726
                     /label="tac promoter"
                     /note="strong E. coli promoter; hybrid between the trp and
                     lac UV5 promoters"
     protein_bind    4727..4751
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             4773..4795
                     /label="RBS"
                     /note="efficient ribosome binding site from bacteriophage
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             4818..5141
                     /label="TrxA"
                     /note="E. coli thioredoxin"
     CDS             5166..5612
                     /label="MNase"
                     /note="micrococcal nuclease from Staphylococcus aureus"
     CDS             5613..5630
                     /label="6xHis"
                     /note="6xHis affinity tag"
     regulatory      5690..5847
                     /label="rrnB terminator"
                     /note="rrnB terminator"
                     /regulatory_class="terminator"
     terminator      complement(6180..6227)
                     /label="T7 terminator"
                     /note="transcription terminator for bacteriophage T7 RNA
                     polymerase"
     CDS             complement(6294..6311)
                     /label="6xHis"
                     /note="6xHis affinity tag"
     CDS             complement(6324..7034)
                     /label="lambda repressor (ts)"
                     /note="temperature-sensitive variant of the phage lambda
                     repressor"
     regulatory      7082..7109
                     /label="lambda PR"
                     /note="lambda PR"
                     /regulatory_class="promoter"
     CDS             7138..7410
                     /gene="E"
                     /label="Lysis protein E"
                     /note="Lysis protein E from Enterobacteria phage phiX174.
                     Accession#: P03639"
ORIGIN
        1 ggccgcaagc ttgtcaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
       61 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
      121 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
      181 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
      241 agcgcaacgc aattaatgta agttagctca ctcattaggc accgggatct cgaccgatgc
      301 ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg actatcgtcg
      361 ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg gcagcgctct
      421 gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc ctgtcgcttg
      481 cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc gccaccaaac
      541 gtttcggcga gaagcaggcc attatcgccg gcatggcggc cccacgggtg cgcatgatcg
      601 tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt tagcagaatg
      661 aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct gcgacctgag
      721 caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag
      781 cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta ccctgtggaa
      841 cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt ctctggtccc
      901 gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg gcatgttcat
      961 catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt acccccatga
     1021 acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca
     1081 tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac gagctggacg
     1141 cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca
     1201 gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
     1261 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
     1321 cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt
     1381 atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatatgcgg
     1441 tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggcgctc ttccgcttcc
     1501 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     1561 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     1621 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     1681 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     1741 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     1801 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     1861 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     1921 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     1981 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     2041 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     2101 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     2161 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     2221 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     2281 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgactaga
     2341 ggcgaattga cataagcctg ttcggttcgt aaactgtaat gcaagtagcg tatgcgctca
     2401 cgcaactggt ccagaacctt gaccgaacgc agcggtggta acggcgcagt ggcggttttc
     2461 atggcttgtt atgactgttt ttttgtacag tctatgcctc gggcatccaa gcagcaagcg
     2521 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagcaa
     2581 cgatgttacg cagcagggca gtcgccctaa aacaaagtta ggtggctcaa gtatgggcat
     2641 cattcgcaca tgtaggctcg gccctgacca agtcaaatcc atgcgggctg ctcttgatct
     2701 tttcggtcgt gagttcggag acgtagccac ctactcccaa catcagccgg actccgatta
     2761 cctcgggaac ttgctccgta gtaagacatt catcgcgctt gctgccttcg accaagaagc
     2821 ggttgttggc gctctcgcgg cttacgttct gcccaggttt gagcagccgc gtagtgagat
     2881 ctatatctat gatctcgcag tctccggcga gcaccggagg cagggcattg ccaccgcgct
     2941 catcaatctc ctcaagcatg aggccaacgc gcttggtgct tatgtgatct acgtgcaagc
     3001 agattacggt gacgatcccg cagtggctct ctatacaaag ttgggcatac gggaagaagt
     3061 gatgcacttt gatatcgacc caagtaccgc cacctaacaa ttcgttcaag ccgagatcgg
     3121 cttcccggcc gcaaactatt aactggcgaa ctacttactc tagcttccat ctcatgagcg
     3181 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     3241 gaaaagtgcc acctgaaatt gtaaacgtta atattggatc cagatctcat gcaccattcc
     3301 ttgcggcggc ggtgctcaac gcctcaacct actactgggc tgcttcctaa tgcaggagtc
     3361 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta
     3421 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
     3481 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
     3541 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
     3601 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
     3661 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
     3721 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
     3781 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
     3841 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
     3901 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
     3961 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
     4021 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
     4081 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
     4141 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
     4201 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
     4261 cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
     4321 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
     4381 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
     4441 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     4501 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     4561 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc
     4621 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
     4681 gtcttcttta tcatgcattg acaattaatc atcggctcgt ataatgggaa ttgtgagcgg
     4741 ataacaattc cacacaggcc tctagaaata attttgttta actttaagaa ggagaggagg
     4801 tatataccat ggggtacagc gataaaatta ttcacctgac tgacgacagt tttgacacgg
     4861 atgtactcaa agcggacggg gcgatcctcg tcgatttctg ggcagagtgg tgcggtccgt
     4921 gcaaaatgat cgccccgatt ctggatgaaa tcgctgacga atatcagggc aaactgaccg
     4981 ttgcaaaact gaacatcgat caaaaccctg gcactgcgcc gaaatatggc atccgtggta
     5041 tcccgactct gctgctgttc aaaaacggtg aagtggcggc aaccaaagtg ggtgcactgt
     5101 ctaaaggtca gttgaaagag ttcctcgacg ctaacctggc cggttccggt tccggtcaca
     5161 ccatggctac ctctaccaaa aaactgcaca aagaaccggc taccctgatc aaagctatcg
     5221 acggtgacac cgttaaactg atgtataaag gccagccgat gaccttccgt ctgctgctgg
     5281 ttgacacccc ggaaaccaaa cacccgaaaa aaggtgttga aaagtacggt ccggaagctt
     5341 ctgctttcac caaaaaaatg gttgaaaacg ctaaaaaaat cgaagttgaa ttcgacaaag
     5401 gtcagcgtac cgacaaatac ggtcgtggtc tggcttacat ctacgctgac ggtaaaatgg
     5461 ttaacgaagc tctggttcgt cagggtctgg ctaaagttgc ttacgtttac aaaccgaaca
     5521 acacccacga acagcacctg cgtaaatctg aagctcaggc taaaaaagaa aaactgaaca
     5581 tctggtctga agacaacgct gactctggtc agcaccatca ccatcatcac tgatgatgag
     5641 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccact gcctggcggc
     5701 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
     5761 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
     5821 aaaggctcag tcgaaagact gggccttcgc tgagcaataa ctagcataac cccttggggc
     5881 ctctaaacct gcaggaattc gtcgaccact acgtgaacca tcaccctaat caagtttttt
     5941 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     6001 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     6061 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     6121 taatgcgccg ctacagggcg cgtcccattc gccaatccgg atatagttcc tcctttcagc
     6181 aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt atgctagtta ttgctcagcg
     6241 gtggcagcag ccaactcagc ttcctttcgg gctttgttag cagccggatc tcagtggtgg
     6301 tggtggtggt gctcgagcgg tcagccaaac gtctcttcag gccactgact agcgataact
     6361 ttccccacaa cggaacaact ctcattgcat gggatcattg ggtactgtgg gtttagtggt
     6421 tgtaaaaaca cctgaccgct atccctgatc agtttcttga aggtaaactc atcaccccca
     6481 agtctggcta tgcagaaatc acctggctca acagcctgct cagggtcaac gagaattaac
     6541 attccgtcag gaaagcttgg cttggagcct gttggtgcgg tcatggaatt accttcaacc
     6601 tcaagccaga atgcagaatc actggctttt ttggttgtgc ttacccatct ctccgcatca
     6661 cctttggtaa aggttctaag cttaggtgag aacatccctg cctgaacatg agaaaaaaca
     6721 gggtactcat actcacttct aagtgacggc tgcatactaa ccgcttcata catctcgtag
     6781 atttctctgg cgattgaagg gctaaattct tcaacgctaa ctttgagaat ttttgtaagc
     6841 aatgcggcgt tataagcatt taatgcattg atgccattaa ataaagcacc aacgcctgac
     6901 tgccccatcc ccatcttgtc tgcgacagat tcctgggata agccaagttc atttttcttt
     6961 ttttcataaa ttgctttaag gcgacgtgcg tcctcaagct gctcttgtgt taatggtttc
     7021 ttttttgtgc tcatacgtta aatctatcac cgcaagggat aaatatctaa caccgtgcgt
     7081 gttgactatt ttacctctgg cggtgataat ggttgcatgt actaaggagg taaacatatg
     7141 gtacgctgga ctttgtggga taccctcgct ttcctgctcc tgttgagttt attgctgccg
     7201 tcattgctta ttatgttcat cccgtcaaca ttcaaacggc ctgtctcatc atggaaggcg
     7261 ctgaatttac ggaaaacatt attaatggcg tcgagcgtcc ggttaaagcc gctgaattgt
     7321 tcgcgcttac cttgcgtgta cgcgcaggaa acactgacgt tcttgctgac gcagaagaaa
     7381 acgtgcgtca aaaattacgt gcagaaggag tgaaaggaaa aaagc
//
