LOCUS       V013553                 5610 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V013553
VERSION     V013553
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     CDS             613..1320
                     /label="mCherry"
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             1387..2265
                     /gene="NSS"
                     /label="Non-structural protein NS-S"
                     /note="Non-structural protein NS-S from SFTS phlebovirus
                     (isolate SFTSV/Human/China/HB29/2010). Accession#:
                     A0A0B5AC19"
     polyA_signal    2398..2519
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(2526..2981)
                     /direction=LEFT
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        3008..3112
                     /label="AmpR promoter"
     promoter        3114..3471
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     CDS             3506..4297
                     /label="NeoR/KanR"
                     /note="aminoglycoside phosphotransferase"
     polyA_signal    4532..4579
                     /label="HSV TK poly(A) signal"
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4908..5496
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc
      661 atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc
      721 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt
      781 ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc
      841 tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc
      901 aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc
      961 tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc
     1021 gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac
     1081 cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc
     1141 cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
     1201 gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg
     1261 gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag
     1321 tccggactca gatctcgagc tcaagcttcg aattctgcag tcgacggtac cggaggaggg
     1381 ggaagcatgt cactgagcaa atgctccaac gttgacctca aatctgttgc aatgaatgcc
     1441 aacacagtca ggcttgagcc atctctagga gagtacccca ctcttaggag ggacctcgtt
     1501 gaatgctctt gcagtgtgtt gactctgtca atggtcaaga ggatgggcaa gatgaccaac
     1561 acagtatggt tgtttggcaa ccctaaaaat cctcttcacc agctcgagcc tggacttgag
     1621 cagctattgg acatgtacta caaggatatg aggtgctact cccagagaga gctgagtgct
     1681 cttaggtggc ctagtgggaa gccatctgta tggttcctac aggcagctca tatgttcttc
     1741 tccatcaaga acagctgggc aatggaaact gggagagaga actggcgggg cctcttccac
     1801 aggataacaa aaggccgaag gtatcttttt gaaggggaca tgatattgga ttctctagaa
     1861 gccatagaga agcgaaggct tagacttggg ttacctgata tcctaataac tggactgtcc
     1921 ccaattctgg atgtggccct ccttcagata gagtcacttg caaggctaag aggcatgagc
     1981 ttgaaccacc acttgttcac ttcttcctca ttgcgtaagc ctctgttaga ctgttgggac
     2041 ttctttatcc ctatccgcaa aaagaagaca gatggctcat acagtgtctt ggatgaggat
     2101 gatgagcctg gggtccttca aggttaccca tatctgatgg cacactattt aaacaggtgc
     2161 ccattccaca atctcatcag gtttgatgag gagctgagaa ctgcagccct gaacaccatc
     2221 tggggaagag attggccagc cattggtgac cccccgaagg aggtctaagg atccaccgga
     2281 tctagataac tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     2341 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     2401 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     2461 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa
     2521 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     2581 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     2641 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     2701 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     2761 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     2821 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     2881 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     2941 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     3001 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3061 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtcctgaggc
     3121 ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
     3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
     3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
     3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa agatcgatca
     3481 agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc
     3541 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
     3601 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
     3661 cctgtccggt gccctgaatg aactgcaaga cgaggcagcg cggctatcgt ggctggccac
     3721 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
     3781 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
     3841 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
     3901 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
     3961 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
     4021 caggctcaag gcgagcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg
     4081 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
     4141 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
     4201 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
     4261 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa
     4321 atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc
     4381 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     4441 ggggatctca tgctggagtt cttcgcccac cctaggggga ggctaactga aacacggaag
     4501 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
     4561 ggtgttgggt cgtttgttca taaacgcggg gttcggtccc agggctggca ctctgtcgat
     4621 accccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc cccaccccac
     4681 cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc aggccctgcc
     4741 atagcctcag gttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     4801 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     4861 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     4921 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     4981 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     5041 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     5101 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     5161 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     5221 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     5281 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     5341 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     5401 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     5461 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     5521 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     5581 tctgtggata accgtattac cgccatgcat
//
