LOCUS       V015113                15307 bp    DNA     circular SYN 01-JAN-1980
DEFINITION  Exported.
ACCESSION   V015113
VERSION     V015113
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 15307)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..15307
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        238..617
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        618..820
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     LTR             835..1015
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1680..1913
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             2098..2142
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             2291..2332
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2440..2557
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     promoter        2608..2848
                     /label="U6 promoter"
                     /note="RNA polymerase III promoter for human U6 snRNA"
     misc_RNA        4738..4813
                     /label="gRNA scaffold"
                     /note="guide RNA scaffold for the Streptococcus pyogenes
                     CRISPR/Cas9 system"
     promoter        4875..5086
                     /label="EF-1-alpha core promoter"
                     /note="core promoter for human elongation factor
                     EF-1-alpha"
     CDS             5151..9251
                     /label="dCas9"
                     /note="catalytically dead mutant of the Cas9 endonuclease
                     from the Streptococcus pyogenes Type II CRISPR/Cas system"
     CDS             9306..9326
                     /label="SV40 NLS"
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
     CDS             9351..9500
                     /label="VP64"
                     /note="tetrameric repeat of the minimal activation domain
                     of herpes simplex virus VP16 (Beerli et al., 1998)"
     CDS             9522..9575
                     /label="T2A"
                     /note="2A peptide from Thosea asigna virus capsid protein"
     CDS             9576..9971
                     /label="BSD"
                     /note="blasticidin S deaminase"
     CDS             complement(10470..10481)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     LTR             11112..11292
                     /label="5' LTR (truncated)"
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     polyA_signal    11324..11548
                     /label="bGH poly(A) signal"
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      11594..12022
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        12036..12365
                     /label="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     promoter        12413..12460
                     /label="EM7 promoter"
                     /note="synthetic bacterial promoter"
     CDS             12479..12850
                     /label="BleoR"
                     /note="antibiotic-binding protein"
     polyA_signal    12983..13116
                     /label="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        complement(13201..13231)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(13246..13267)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(13555..14143)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(14317..15174)
                     /label="AmpR"
                     /note="beta-lactamase"
     promoter        complement(15175..15279)
                     /label="AmpR promoter"
     misc_feature    15307
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccgag ggcctatttc ccatgattcc ttcatatttg
     2641 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
     2701 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
     2761 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
     2821 ttggctttat atatcttgtg gaaaggacga aacaccggag acggttgtaa atgagcacac
     2881 aaaatacaca tgctaaaata ttatattcta tgacctttat aaaatcaacc aaaatcttct
     2941 ttttaataac tttagtatca ataattagaa tttttatgtt cctttttgca aacttttaat
     3001 aaaaatgagc aaaataaaaa aacgctagtt ttagtaactc gcgttgtttt cttcaccttt
     3061 aataatagct actccaccac ttgttcctaa gcggtcagct cctgcttcaa tcattttttg
     3121 agcatcttca aatgttctaa ctccaccagc tgctttaact aaagcattgt ctttaacaac
     3181 tgacttcatt agtttaacat cttcaaatgt tgcacctgat tttgaaaatc ctgttgatgt
     3241 tttaacaaat tctaatccag cttcaacagc tatttcacaa gctttcatga tttcttcttt
     3301 tgttaataaa caattttcca taatacattt aacaacatgt gatccagctg ctttttttac
     3361 agctttcatg tcttctaaaa ctaattcata atttttgtct tttaatgcac caatatttaa
     3421 taccatatca atttctgttg caccatcttt aattgcttca gaaacttcga atgcttttgt
     3481 agctgttgtg catgcaccta gaggaaaacc tacaacattt gttattccta catttgtgcc
     3541 ttttaataat tctttacaat agcttgttca atatgaatta acacaaactg ttgcaaaatc
     3601 aaattcaatt gcttcatcac ataattgttt aatttcagct ttcgtagcat cttgttttaa
     3661 taatgtgtga tctatatatt tgtttagttt cattttttct cctatatatt catttttaat
     3721 tttaattctt taataatttc gtctacttta actttagcgt tttgaacaga ttcaccaaca
     3781 cctataaaat aaatttttag tttaggttca gttccacttg ggcgaacagc aaatcatgac
     3841 ttatcttcta aataaaattt tagtaagtct tgtcctggca tattatacat tccatcgatg
     3901 tagtcttcaa cattaacaac tttaagtcca gcaatttgag ttaagggtgt tgctctcaat
     3961 gatttcatta atggttcaat ttttaatttc ttttcttctg gtttaaaatt caagtttaaa
     4021 gtgaaagtgt aatatgcacc catttcttta aataaatctt ctaaatagtc tactaatgtt
     4081 ttattttgtt ttttataaaa tcaagcagcc tctgctatta atatagaagc ttgtattcca
     4141 tctttatctc tagctgagtc atcaattaca tatccataac tttcttcata agcaaaaaca
     4201 aaatttaatc cgttatcttc ttctttagca atttctctac ccattcattt aaatccagtt
     4261 aaagttttta caatattaac tccatatttt tcatgagcga ttctatcacc caaatcactt
     4321 gttacaaaac ttgaatatag agccggattt tttggaatgc tatttaagcg ttttagattt
     4381 gataattttc aatcaattaa aattggtcct gtttgatttc catctaatct tacaaaatga
     4441 ccatcatgtt ttattgccat tccaaatctg tcagcatctg ggtcattcat aataataata
     4501 tctgcatcat gtttaatacc atattcaagc ggtatttttc atgcaggatc aaattctgga
     4561 tttggattta caacattttt aaatgtttca tcttcaaatg catgctcttc aacctcaata
     4621 acgttatatc ctgattcacg taatattttt ggggtaaatt tagttcctgt tccattaact
     4681 gcgctaaaaa taatttttaa atctttttta gcttcttgct cttttttgta cgtctctgtt
     4741 ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg aaaaagtggc
     4801 accgagtcgg tgcttttttg aattcgctag ctaggtcttg aaaggagtgg gaattggctc
     4861 cggtgcccgt cagtgggcag agcgcacatc gcccacagtc cccgagaagt tggggggagg
     4921 ggtcggcaat tgatccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt
     4981 cgtgtactgg ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt
     5041 cgccgtgaac gttctttttc gcaacgggtt tgccgccaga acacaggcgt acggccacca
     5101 tgaaaaggcc ggcggccacg aaaaaggccg gccaggcaaa aaagaaaaag gacaagaagt
     5161 acagcatcgg cctggccatc ggcaccaact ctgtgggctg ggccgtgatc accgacgagt
     5221 acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga
     5281 agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc acccggctga
     5341 agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat ctgcaagaga
     5401 tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg gaagagtcct
     5461 tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac atcgtggacg
     5521 aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa ctggtggaca
     5581 gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg atcaagttcc
     5641 ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg gacaagctgt
     5701 tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc aacgccagcg
     5761 gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc
     5821 tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggcaacctg attgccctga
     5881 gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat gccaaactgc
     5941 agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag atcggcgacc
     6001 agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg ctgagcgaca
     6061 tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg atcaagagat
     6121 acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg
     6181 agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc tacattgacg
     6241 gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa aagatggacg
     6301 gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag cagcggacct
     6361 tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc attctgcggc
     6421 ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag aagatcctga
     6481 ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga ttcgcctgga
     6541 tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg gtggacaagg
     6601 gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac ctgcccaacg
     6661 agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat aacgagctga
     6721 ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc ggcgagcaga
     6781 aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga
     6841 aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag
     6901 atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc aaggacaagg
     6961 acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg accctgacac
     7021 tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg
     7081 acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg ctgagccgga
     7141 agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat ttcctgaagt
     7201 ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc ctgaccttta
     7261 aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac gagcacattg
     7321 ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg aaggtggtgg
     7381 acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc gaaatggcca
     7441 gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg aagcggatcg
     7501 aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc
     7561 agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat atgtacgtgg
     7621 accaggaact ggacatcaac cggctgtccg actacgatgt ggaccacatc gtgcctcaga
     7681 gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac aaggcccggg
     7741 gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac tactggcggc
     7801 agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc aaggccgaga
     7861 gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg gtggaaaccc
     7921 ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact aagtacgacg
     7981 agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg
     8041 atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac caccacgccc
     8101 acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg
     8161 aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg atcgccaaga
     8221 gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac atcatgaact
     8281 ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct ctgatcgaga
     8341 caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc accgtgcgga
     8401 aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct
     8461 tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc agaaagaagg
     8521 actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat tctgtgctgg
     8581 tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg
     8641 ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt ctggaagcca
     8701 agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac tccctgttcg
     8761 agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag aagggaaacg
     8821 aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac tatgagaagc
     8881 tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag cacaagcact
     8941 acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc ctggccgacg
     9001 ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc atcagagagc
     9061 aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct gccgccttca
     9121 agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag gtgctggacg
     9181 ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac ctgtctcagc
     9241 tgggaggcga cagcgctgga ggaggtggaa gcggaggagg aggaagcgga ggaggaggta
     9301 gcggacctaa gaaaaagagg aaggtggcgg ccgctggatc cggacgggct gacgcattgg
     9361 acgattttga tctggatatg ctgggaagtg acgccctcga tgattttgac cttgacatgc
     9421 ttggttcgga tgcccttgat gactttgacc tcgacatgct cggcagtgac gcccttgatg
     9481 atttcgacct ggacatgctg attaactgta caggcagtgg agagggcaga ggaagtctgc
     9541 taacatgcgg tgacgtcgag gagaatcctg gcccaatggc caagcctttg tctcaagaag
     9601 aatccaccct cattgaaaga gcaacggcta caatcaacag catccccatc tctgaagact
     9661 acagcgtcgc cagcgcagct ctctctagcg acggccgcat cttcactggt gtcaatgtat
     9721 atcattttac tgggggacct tgtgcagaac tcgtggtgct gggcactgct gctgctgcgg
     9781 cagctggcaa cctgacttgt atcgtcgcga tcggaaatga gaacaggggc atcttgagcc
     9841 cctgcggacg gtgccgacag gtgcttctcg atctgcatcc tgggatcaaa gccatagtga
     9901 aggacagtga tggacagccg acggcagttg ggattcgtga attgctgccc tctggttatg
     9961 tgtgggaggg ctaagaattc gatatcaagc ttatcggtaa tcaacctctg gattacaaaa
    10021 tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg
    10081 ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct
    10141 tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg
    10201 gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct
    10261 gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg
    10321 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
    10381 tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc
    10441 tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc
    10501 gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc
    10561 ggatctccct ttgggccgcc tccccgcatc gataccgtcg acctcgagac ctagaaaaac
    10621 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
    10681 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
    10741 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
    10801 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
    10861 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
    10921 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
    10981 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
    11041 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
    11101 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
    11161 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
    11221 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
    11281 aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
    11341 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
    11401 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
    11461 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
    11521 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
    11581 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
    11641 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
    11701 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
    11761 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
    11821 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
    11881 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
    11941 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
    12001 taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt
    12061 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
    12121 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
    12181 agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
    12241 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
    12301 cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
    12361 gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac gtgttgacaa
    12421 ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga actaaaccat
    12481 ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg gagcggtcga
    12541 gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact tcgccggtgt
    12601 ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg tgccggacaa
    12661 caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt ggtcggaggt
    12721 cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg gcgagcagcc
    12781 gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact tcgtggccga
    12841 ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct atgaaaggtt
    12901 gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat
    12961 gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt acaaataaag
    13021 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
    13081 gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta gctagagctt
    13141 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    13201 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    13261 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    13321 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
    13381 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
    13441 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
    13501 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
    13561 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
    13621 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
    13681 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
    13741 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
    13801 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
    13861 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
    13921 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
    13981 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
    14041 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
    14101 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
    14161 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
    14221 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
    14281 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
    14341 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
    14401 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
    14461 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
    14521 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
    14581 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
    14641 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
    14701 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
    14761 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
    14821 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
    14881 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
    14941 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
    15001 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
    15061 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
    15121 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
    15181 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
    15241 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
    15301 acctgac
//
