LOCUS       40924_27553       11574 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector placZ.attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11574)
  AUTHORS   Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
  TITLE     A versatile platform for creating a comprehensive UAS-ORFeome 
            library in Drosophila
  JOURNAL   Development 140 (11), 2434-2442 (2013)
  PUBMED    23637332
REFERENCE   2  (bases 1 to 11574)
  AUTHORS   Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2013) Institute of Molecular Life Sciences, 
            University of Zurich, Winterthurerstrasse 190, Zurich 8057, 
            Switzerland
REFERENCE   3  (bases 1 to 11574)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11574)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development"; date: "2013"; volume: "140"; issue: "11"; pages: 
            "2434-2442"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2013) Institute of Molecular Life Sciences, University of 
            Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11574
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=multiple cloning site
                     /note="multiple cloning site"
     promoter        74..312
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     CDS             381..401
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             411..3455
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     intron          3594..3659
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3789..3809
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    4081..4215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    4244..4313
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(4532..4550)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4571..4587)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4595..4611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4619..4649)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4664..4685)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4973..5561)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5735..6592)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6593..6697)
                     /label=AmpR promoter
     rep_origin      complement(6723..7178)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7320..7336
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7343..7361
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7387..11522
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
     CDS             join(7865..7936,8338..8611,8686..9340,9402..9717,
                     9938..10069,10140..10754)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This ia a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    11529..11562
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aagcttcgca ggcctcgcac cggtgaattc gttaacagat ctgcggccgc ggctcgaggg
       61 tacctctaga gcgccggagt ataaatagag gcgcttcgtc tacggagcga caattcaatt
      121 caaacaagca aagtgaacac gtcgctaagc gaaagctaag caaataaaca agcgcagctg
      181 aacaagctaa acaatctgca gtaaagtgca agttaaagtg aatcaattaa aagtaaccag
      241 caaccaagta aatcaactgc aactactgaa atctgccaag aagtaattat tgaatacaag
      301 aagagaactc tgaataggga attgggaatt aattcccgag aaagaagaag aactcacacc
      361 caatgcctgc tattggaatc cccaagaaga agcgcaaggt gaaagatccc gtcgttttac
      421 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
      481 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
      541 gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
      601 gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
      661 tgcacggtta cgatgcgccc atctacacca acgtgaccta tcccattacg gtcaatccgc
      721 cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
      781 gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttaactcg gcgtttcatc
      841 tgtggtgcaa cgggcgctgg gtcggttacg gccaggacag tcgtttgccg tctgaatttg
      901 acctgagcgc atttttacgc gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga
      961 gtgacggcag ttatctggaa gatcaggata tgtggcggat gagcggcatt ttccgtgacg
     1021 tctcgttgct gcataaaccg actacacaaa tcagcgattt ccatgttgcc actcgcttta
     1081 atgatgattt cagccgcgct gtactggagg ctgaagttca gatgtgcggc gagttgcgtg
     1141 actacctacg ggtaacagtt tctttatggc agggtgaaac gcaggtcgcc agcggcaccg
     1201 cgcctttcgg cggtgaaatt atcgatgagc gtggtggtta tgccgatcgc gtcacactac
     1261 gtctgaacgt cgaaaacccg aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg
     1321 tggttgaact gcacaccgcc gacggcacgc tgattgaagc agaagcctgc gatgtcggtt
     1381 tccgcgaggt gcggattgaa aatggtctgc tgctgctgaa cggcaagccg ttgctgattc
     1441 gaggcgttaa ccgtcacgag catcatcctc tgcatggtca ggtcatggat gagcagacga
     1501 tggtgcagga tatcctgctg atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt
     1561 atccgaacca tccgctgtgg tacacgctgt gcgaccgcta cggcctgtat gtggtggatg
     1621 aagccaatat tgaaacccac ggcatggtgc caatgaatcg tctgaccgat gatccgcgct
     1681 ggctaccggc gatgagcgaa cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga
     1741 gtgtgatcat ctggtcgctg gggaatgaat caggccacgg cgctaatcac gacgcgctgt
     1801 atcgctggat caaatctgtc gatccttccc gcccggtgca gtatgaaggc ggcggagccg
     1861 acaccacggc caccgatatt atttgcccga tgtacgcgcg cgtggatgaa gaccagccct
     1921 tcccggctgt gccgaaatgg tccatcaaaa aatggctttc gctacctgga gagacgcgcc
     1981 cgctgatcct ttgcgaatac gcccacgcga tgggtaacag tcttggcggt ttcgctaaat
     2041 actggcaggc gtttcgtcag tatccccgtt tacagggcgg cttcgtctgg gactgggtgg
     2101 atcagtcgct gattaaatat gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt
     2161 ttggcgatac gccgaacgat cgccagttct gtatgaacgg tctggtcttt gccgaccgca
     2221 cgccgcatcc agcgctgacg gaagcaaaac accagcagca gtttttccag ttccgtttat
     2281 ccgggcaaac catcgaagtg accagcgaat acctgttccg tcatagcgat aacgagctcc
     2341 tgcactggat ggtggcgctg gatggtaagc cgctggcaag cggtgaagtg cctctggatg
     2401 tcgctccaca aggtaaacag ttgattgaac tgcctgaact accgcagccg gagagcgccg
     2461 ggcaactctg gctcacagta cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg
     2521 ggcacatcag cgcctggcag cagtggcgtc tggcggaaaa cctcagtgtg acgctccccg
     2581 ccgcgtccca cgccatcccg catctgacca ccagcgaaat ggatttttgc atcgagctgg
     2641 gtaataagcg ttggcaattt aaccgccagt caggctttct ttcacagatg tggattggcg
     2701 ataaaaaaca actgctgacg ccgctgcgcg atcagttcac ccgtgcaccg ctggataacg
     2761 acattggcgt aagtgaagcg acccgcattg accctaacgc ctgggtcgaa cgctggaagg
     2821 cggcgggcca ttaccaggcc gaagcagcgt tgttgcagtg cacggcagat acacttgctg
     2881 atgcggtgct gattacgacc gctcacgcgt ggcagcatca ggggaaaacc ttatttatca
     2941 gccggaaaac ctaccggatt gatggtagtg gtcaaatggc gattaccgtt gatgttgaag
     3001 tggcgagcga tacaccgcat ccggcgcgga ttggcctgaa ctgccagctg gcgcaggtag
     3061 cagagcgggt aaactggctc ggattagggc cgcaagaaaa ctatcccgac cgccttactg
     3121 ccgcctgttt tgaccgctgg gatctgccat tgtcagacat gtataccccg tacgtcttcc
     3181 cgagcgaaaa cggtctgcgc tgcgggacgc gcgaattgaa ttatggccca caccagtggc
     3241 gcggcgactt ccagttcaac atcagccgct acagtcaaca gcaactgatg gaaaccagcc
     3301 atcgccatct gctgcacgcg gaagaaggca catggctgaa tatcgacggt ttccatatgg
     3361 ggattggtgg cgacgactcc tggagcccgt cagtatcggc ggaattacag ctgagcgccg
     3421 gtcgctacca ttaccagttg gtctggtgtc aaaaataata ataaccgggc aggccatgtc
     3481 tgcccgtatt tcgcgtaagg aaatccctcg actagaggat ctttgtgaag gaaccttact
     3541 tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata
     3601 taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga
     3661 ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc
     3721 tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt
     3781 ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc
     3841 taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca
     3901 ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat ttgatgtata
     3961 gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt acttgcttta
     4021 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt
     4081 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
     4141 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
     4201 tatcatgtct ggatccacta gtgtcgacga tgtaggtcac ggtctcgaag ccgcggtgcg
     4261 ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc cacctcaccc atctggtcca
     4321 tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc gaacgcgcgg cgcaccggga
     4381 agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt gagcacggga cgtgcgacgg
     4441 cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt ctcgacggtc acggcgggca
     4501 tgtcgacact agttctagcc agcttttgtt ccctttagtg agggttaatt tcgagcttgg
     4561 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca
     4621 acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca
     4681 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc
     4741 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt
     4801 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact
     4861 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag
     4921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     4981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     5041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     5101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     5161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     5221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     5281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     5341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     5401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     5461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     5521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     5581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     5641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     5701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     5761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     5821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     5881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     5941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     6001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     6061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     6121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     6181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     6241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     6301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     6361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     6421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     6481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     6541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     6601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     6661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     6721 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
     6781 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
     6841 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
     6901 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
     6961 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
     7021 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
     7081 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
     7141 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
     7201 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
     7261 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
     7321 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
     7381 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
     7441 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
     7501 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
     7561 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
     7621 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
     7681 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     7741 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     7801 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     7861 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     7921 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     7981 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     8041 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     8101 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     8161 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     8221 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     8281 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     8341 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     8401 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     8461 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     8521 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     8581 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     8641 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     8701 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     8761 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     8821 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     8881 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     8941 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     9001 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     9061 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     9121 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     9181 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     9241 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     9301 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     9361 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     9421 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     9481 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     9541 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     9601 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     9661 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     9721 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     9781 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     9841 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     9901 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     9961 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
    10021 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
    10081 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
    10141 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
    10201 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
    10261 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
    10321 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
    10381 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
    10441 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
    10501 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
    10561 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
    10621 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
    10681 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
    10741 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
    10801 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
    10861 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
    10921 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
    10981 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
    11041 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
    11101 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
    11161 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
    11221 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
    11281 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
    11341 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
    11401 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
    11461 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
    11521 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atcc
//
