LOCUS       40924_27548       11313 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector placZ-2.attB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11313)
  AUTHORS   Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
  TITLE     A versatile platform for creating a comprehensive UAS-ORFeome 
            library in Drosophila
  JOURNAL   Development 140 (11), 2434-2442 (2013)
  PUBMED    23637332
REFERENCE   2  (bases 1 to 11313)
  AUTHORS   Bischof J, Bjorklund M, Furger E, Schertel C, Taipale J, Basler K.
  TITLE     Direct Submission
  JOURNAL   Submitted (13-APR-2013) Institute of Molecular Life Sciences, 
            University of Zurich, Winterthurerstrasse 190, Zurich 8057, 
            Switzerland
REFERENCE   3  (bases 1 to 11313)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11313)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development"; date: "2013"; volume: "140"; issue: "11"; pages: 
            "2434-2442"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (13-APR-2013) Institute of Molecular Life Sciences, University of 
            Zurich, Winterthurerstrasse 190, Zurich 8057, Switzerland"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..11313
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..70
                     /label=multiple cloning site
                     /note="multiple cloning site"
     CDS             120..140
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     CDS             150..3194
                     /codon_start=1
                     /label=lacZ
                     /note="beta-galactosidase"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     intron          3333..3398
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3528..3548
                     /codon_start=1
                     /label=SV40 NLS
                     /note="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /translation="PKKKRKV"
     polyA_signal    3820..3954
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    3983..4052
                     /label=attB
                     /note="attB site for the phi-C31 integrase (Groth et al.,
                     2000)"
     promoter        complement(4271..4289)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(4310..4326)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4334..4350
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(4358..4388)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(4403..4424)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(4712..5300)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(5474..6331)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(6332..6436)
                     /label=AmpR promoter
     rep_origin      complement(6462..6917)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7059..7075
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7082..7100
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7126..11261
                     /label=white dominant eye marker
                     /note="white dominant eye marker"
     CDS             join(7604..7675,8077..8350,8425..9079,9141..9456,9677..9808,
                     9879..10493)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This ia a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    11268..11301
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 aagcttcgca ggcctcgcac cggtgaattc gttaacagat ctgcggccgc ggctcgaggg
       61 tacctctaga attcccgaga aagaagaaga actcacaccc aatgcctgct attggaatcc
      121 ccaagaagaa gcgcaaggtg aaagatcccg tcgttttaca acgtcgtgac tgggaaaacc
      181 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
      241 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
      301 gctttgcctg gtttccggca ccagaagcgg tgccggaaag ctggctggag tgcgatcttc
      361 ctgaggccga tactgtcgtc gtcccctcaa actggcagat gcacggttac gatgcgccca
      421 tctacaccaa cgtgacctat cccattacgg tcaatccgcc gtttgttccc acggagaatc
      481 cgacgggttg ttactcgctc acatttaatg ttgatgaaag ctggctacag gaaggccaga
      541 cgcgaattat ttttgatggc gttaactcgg cgtttcatct gtggtgcaac gggcgctggg
      601 tcggttacgg ccaggacagt cgtttgccgt ctgaatttga cctgagcgca tttttacgcg
      661 ccggagaaaa ccgcctcgcg gtgatggtgc tgcgctggag tgacggcagt tatctggaag
      721 atcaggatat gtggcggatg agcggcattt tccgtgacgt ctcgttgctg cataaaccga
      781 ctacacaaat cagcgatttc catgttgcca ctcgctttaa tgatgatttc agccgcgctg
      841 tactggaggc tgaagttcag atgtgcggcg agttgcgtga ctacctacgg gtaacagttt
      901 ctttatggca gggtgaaacg caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta
      961 tcgatgagcg tggtggttat gccgatcgcg tcacactacg tctgaacgtc gaaaacccga
     1021 aactgtggag cgccgaaatc ccgaatctct atcgtgcggt ggttgaactg cacaccgccg
     1081 acggcacgct gattgaagca gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa
     1141 atggtctgct gctgctgaac ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc
     1201 atcatcctct gcatggtcag gtcatggatg agcagacgat ggtgcaggat atcctgctga
     1261 tgaagcagaa caactttaac gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt
     1321 acacgctgtg cgaccgctac ggcctgtatg tggtggatga agccaatatt gaaacccacg
     1381 gcatggtgcc aatgaatcgt ctgaccgatg atccgcgctg gctaccggcg atgagcgaac
     1441 gcgtaacgcg aatggtgcag cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg
     1501 ggaatgaatc aggccacggc gctaatcacg acgcgctgta tcgctggatc aaatctgtcg
     1561 atccttcccg cccggtgcag tatgaaggcg gcggagccga caccacggcc accgatatta
     1621 tttgcccgat gtacgcgcgc gtggatgaag accagccctt cccggctgtg ccgaaatggt
     1681 ccatcaaaaa atggctttcg ctacctggag agacgcgccc gctgatcctt tgcgaatacg
     1741 cccacgcgat gggtaacagt cttggcggtt tcgctaaata ctggcaggcg tttcgtcagt
     1801 atccccgttt acagggcggc ttcgtctggg actgggtgga tcagtcgctg attaaatatg
     1861 atgaaaacgg caacccgtgg tcggcttacg gcggtgattt tggcgatacg ccgaacgatc
     1921 gccagttctg tatgaacggt ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg
     1981 aagcaaaaca ccagcagcag tttttccagt tccgtttatc cgggcaaacc atcgaagtga
     2041 ccagcgaata cctgttccgt catagcgata acgagctcct gcactggatg gtggcgctgg
     2101 atggtaagcc gctggcaagc ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt
     2161 tgattgaact gcctgaacta ccgcagccgg agagcgccgg gcaactctgg ctcacagtac
     2221 gcgtagtgca accgaacgcg accgcatggt cagaagccgg gcacatcagc gcctggcagc
     2281 agtggcgtct ggcggaaaac ctcagtgtga cgctccccgc cgcgtcccac gccatcccgc
     2341 atctgaccac cagcgaaatg gatttttgca tcgagctggg taataagcgt tggcaattta
     2401 accgccagtc aggctttctt tcacagatgt ggattggcga taaaaaacaa ctgctgacgc
     2461 cgctgcgcga tcagttcacc cgtgcaccgc tggataacga cattggcgta agtgaagcga
     2521 cccgcattga ccctaacgcc tgggtcgaac gctggaaggc ggcgggccat taccaggccg
     2581 aagcagcgtt gttgcagtgc acggcagata cacttgctga tgcggtgctg attacgaccg
     2641 ctcacgcgtg gcagcatcag gggaaaacct tatttatcag ccggaaaacc taccggattg
     2701 atggtagtgg tcaaatggcg attaccgttg atgttgaagt ggcgagcgat acaccgcatc
     2761 cggcgcggat tggcctgaac tgccagctgg cgcaggtagc agagcgggta aactggctcg
     2821 gattagggcc gcaagaaaac tatcccgacc gccttactgc cgcctgtttt gaccgctggg
     2881 atctgccatt gtcagacatg tataccccgt acgtcttccc gagcgaaaac ggtctgcgct
     2941 gcgggacgcg cgaattgaat tatggcccac accagtggcg cggcgacttc cagttcaaca
     3001 tcagccgcta cagtcaacag caactgatgg aaaccagcca tcgccatctg ctgcacgcgg
     3061 aagaaggcac atggctgaat atcgacggtt tccatatggg gattggtggc gacgactcct
     3121 ggagcccgtc agtatcggcg gaattacagc tgagcgccgg tcgctaccat taccagttgg
     3181 tctggtgtca aaaataataa taaccgggca ggccatgtct gcccgtattt cgcgtaagga
     3241 aatccctcga ctagaggatc tttgtgaagg aaccttactt ctgtggtgtg acataattgg
     3301 acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta agtgtataat
     3361 gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat ggaactgatg
     3421 aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca gaagaaatgc
     3481 catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca aaaaagaaga
     3541 gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg agtcatgctg
     3601 tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa aaagctgcac
     3661 tgctatacaa gaaaattatg gaaaaatatt tgatgtatag tgccttgact agagatcata
     3721 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     3781 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     3841 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     3901 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccactag
     3961 tgtcgacgat gtaggtcacg gtctcgaagc cgcggtgcgg gtgccagggc gtgcccttgg
     4021 gctccccggg cgcgtactcc acctcaccca tctggtccat catgatgaac gggtcgaggt
     4081 ggcggtagtt gatcccggcg aacgcgcggc gcaccgggaa gccctcgccc tcgaaaccgc
     4141 tgggcgcggt ggtcacggtg agcacgggac gtgcgacggc gtcggcgggt gcggatacgc
     4201 ggggcagcgt cagcgggttc tcgacggtca cggcgggcat gtcgacacta gttctagcca
     4261 gcttttgttc cctttagtga gggttaattt cgagcttggc gtaatcatgg tcatagctgt
     4321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
     4381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
     4441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
     4501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
     4561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
     4621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
     4681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
     4741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
     4801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
     4861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
     4921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
     4981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
     5041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
     5101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
     5161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
     5221 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
     5281 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
     5341 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
     5401 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
     5461 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     5521 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     5581 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     5641 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     5701 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     5761 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     5821 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     5881 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     5941 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     6001 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     6061 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     6121 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     6181 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     6241 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     6301 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
     6361 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
     6421 aaataggggt tccgcgcaca tttccccgaa aagtgccacc taaattgtaa gcgttaatat
     6481 tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     6541 aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     6601 agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     6661 cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     6721 gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     6781 gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     6841 ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     6901 gccgctacag ggcgcgtccc attcgccatt caggctgcgc aactgttggg aagggcgatc
     6961 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt
     7021 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt
     7081 gtaatacgac tcactatagg gcgaattggg tacgtaccgg gcccctagta tgtatgtaag
     7141 ttaataaaac ccatttttgc ggaaagtaga taaaaaaaac attttttttt tttactgcac
     7201 tggatatcat tgaacttatc tgatcagttt taaatttact tcgatccaag ggtatttgat
     7261 gtaccaggtt ctttcgatta cctctcactc aaaatgacat tccactcaaa gtcagcgctg
     7321 tttgcctcct tctctgtcca cagaaatatc gccgtctctt tcgccgctgc gtccgctatc
     7381 tctttcgcca ccgtttgtag cgttacgtag cgtcaatgtc cgccttcagt tgcattttgt
     7441 cagcggtttc gtgacgaagc tccaagcggt ttacgccatc aattaaacac aaagtgctgt
     7501 gccaaaactc ctctcgcttc ttatttttgt ttgttttttg agtgattggg gtggtgattg
     7561 gttttgggtg ggtaagcagg ggaaagtgtg aaaaatcccg gcaatgggcc aagaggatca
     7621 ggagctatta attcgcggag gcagcaaaca cccatctgcc gagcatctga acaatgtgag
     7681 tagtacatgt gcatacatct taagttcact tgatctatag gaactgcgat tgcaacatca
     7741 aattgtctgc ggcgtgagaa ctgcgaccca caaaaatccc aaaccgcaat tgcacaaaca
     7801 aatagtgaca cgaaacagat tattctggta gctgttctcg ctatataaga caatttttga
     7861 gatcatatca tgatcaagac atctaaaggc attcattttc gactatattc ttttttacaa
     7921 aaaatataac aaccagatat tttaagctga tcctagatgc acaaaaaata aataaaagta
     7981 taaacctact tcgtaggata cttcggggta ctttttgttc ggggttagat gagcataacg
     8041 cttgtagttg atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag
     8101 agctgcatta accagggctt cgggcaggcc aaaaactacg gcacgctccg gccacccagt
     8161 ccgccggagg actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg
     8221 cacaatatgg acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc
     8281 aaccggacac gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg
     8341 ctcaagaacg gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg
     8401 tccaattacc aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg
     8461 tgatgggcag ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc
     8521 cgcagggcat ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg
     8581 ccaaggagat gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc
     8641 taacggccag ggaacacctg attttccagg ccatggtgcg gatgccacga catctgacct
     8701 atcggcagcg agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc
     8761 agcacacgat catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc
     8821 gtctggcatt cgcctccgag gcactaaccg atccgccgct tctgatctgc gatgagccca
     8881 cctccggact ggactcattt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc
     8941 agaagggcaa gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct
     9001 ttgacaagat ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag
     9061 ccgtcgactt cttttcctag tgagttcgat gtgtttatta agggtatcta gcattacatt
     9121 acatctcaac tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac
     9181 ttttacgtac aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc
     9241 gccaagatat gcgacaattt tgctattagc aaagtagccc gggatatgga gcagttgttg
     9301 gccaccaaaa atttggagaa gccactggag cagccggaga atgggtacac ctacaaggcc
     9361 acctggttca tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa
     9421 ccactcctcg taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa
     9481 tgatataacg cttacaattc ttggaaacaa attcgctaga ttttagttag aattgcctga
     9541 ttccacaccc ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaaaata
     9601 aataaatttc atttaactcg cgaacatgtt gaagatatga atattaatga gatgcgagta
     9661 acattttaat ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact
     9721 cacgcaagtg ggcgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac
     9781 ctttcaaaac gtctttgcca cgataaatgt aagtcttgtt tagaatacat ttgcatatta
     9841 ataatttact aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag
     9901 tttttatgag ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa
     9961 cgattgccga attaccgctt tttctcacag tgccactggt cttcacggcg attgcctatc
    10021 cgatgatcgg actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc
    10081 tggtggccaa tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct
    10141 cgatggcgct gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct
    10201 tcttgaactc gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc
    10261 gttacgccaa cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta
    10321 gctgcacatc gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta
    10381 acttctccgc cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct
    10441 tccgggtgct cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat
    10501 atatccgaaa taactgcttg tttttttttt ttaccattat taccatcgtg tttactgttt
    10561 attgccccct caaaaagcta atgtaattat atttgtgcca ataaaaacaa gatatgacct
    10621 atagaataca agtatttccc cttcgaacat ccccacaagt agactttgga tttgtcttct
    10681 aaccaaaaga cttacacacc tgcatacctt acatcaaaaa ctcgtttatc gctacataaa
    10741 acaccgggat atatttttta tatacatact tttcaaatcg cgcgccctct tcataattca
    10801 cctccaccac accacgtttc gtagttgctc tttcgctgtc tcccacccgc tctccgcaac
    10861 acattcacct tttgttcgac gaccttggag cgactgtcgt tagttccgcg cgattcggtt
    10921 cgctcaaatg gttccgagtg gttcatttcg tctcaataga aattagtaat aaatatttgt
    10981 atgtacaatt tatttgctcc aatatatttg tatatatttc cctcacagct atatttattc
    11041 taatttaata ttatgacttt ttaaggtaat tttttgtgac ctgttcggag tgattagcgt
    11101 tacaatttga actgaaagtg acatccagtg tttgttcctt gtgtagatgc atctcaaaaa
    11161 aatggtgggc ataatagtgt tgtttatata tatcaaaaat aacaactata ataataagaa
    11221 tacatttaat ttagaaaatg cttggatttc actggaacta ggctagcata acttcgtata
    11281 atgtatgcta tacgaagtta tgctagcgga tcc
//
