LOCUS       62056_13630        5477 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5477)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..5477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..423
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     intron          493..625
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        670..688
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             698..1630
                     /codon_start=1
                     /label=Rluc
                     /note="luciferase from the anthozoan coelenterate Renilla 
                     reniformis (sea pansy)"
                     /translation="MTSKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1732..3381
                     /codon_start=1
                     /label=luciferase
                     /note="firefly luciferase"
                     /translation="LEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     polyA_signal    complement(3424..3545)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        3686..3790
                     /label=AmpR promoter
     CDS             3791..4648
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      4822..5410
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatcgatct gcgcagcacc atggcctgaa ataacctctg aaagaggaac ttggttaggt
       61 accttctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg tggaaagtcc
      121 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
      181 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
      241 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
      301 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
      361 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
      421 aaaaagcttg attcttctga cacaacagtc tcgaacttaa gctgcagaag ttggtcgtga
      481 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
      541 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
      601 atccactttg cctttctctc cacaggtgtc cactcccagt tcaattacag ctcttaaggc
      661 tagagtactt aatacgactc actataggct agccaccatg acttcgaaag tttatgatcc
      721 agaacaaagg aaacggatga taactggtcc gcagtggtgg gccagatgta aacaaatgaa
      781 tgttcttgat tcatttatta attattatga ttcagaaaaa catgcagaaa atgctgttat
      841 ttttttacat ggtaacgcgg cttcttctta tttatggcga catgttgtgc cacatattga
      901 gccagtagcg cggtgtatta taccagacct tattggtatg ggcaaatcag gcaaatctgg
      961 taatggttct tataggttac ttgatcatta caaatatctt actgcatggt ttgaacttct
     1021 taatttacca aagaagatca tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca
     1081 ttatagctat gagcatcaag ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga
     1141 tgtgattgaa tcatgggatg aatggcctga tattgaagaa gatattgcgt tgatcaaatc
     1201 tgaagaagga gaaaaaatgg ttttggagaa taacttcttc gtggaaacca tgttgccatc
     1261 aaaaatcatg agaaagttag aaccagaaga atttgcagca tatcttgaac cattcaaaga
     1321 gaaaggtgaa gttcgtcgtc caacattatc atggcctcgt gaaatcccgt tagtaaaagg
     1381 tggtaaacct gacgttgtac aaattgttag gaattataat gcttatctac gtgcaagtga
     1441 tgatttacca aaaatgttta ttgaatcgga cccaggattc ttttccaatg ctattgttga
     1501 aggtgccaag aagtttccta atactgaatt tgtcaaagta aaaggtcttc atttttcgca
     1561 agaagatgca cctgatgaaa tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa
     1621 aaatgaacaa atgtcgacgg atcctttaaa cgggtacggg gtagcagtga ggctcggctg
     1681 ataccccttg ctagtggatg tgatcctgat gttgtaaagc gagcctttga gctcgaagac
     1741 gccaaaaaca taaagaaagg cccggcgcca ttctatcctc tagaggatgg aaccgctgga
     1801 gagcaactgc ataaggctat gaagagatac gccctggttc ctggaacaat tgcttttaca
     1861 gatgcacata tcgaggtgaa catcacgtac gcggaatact tcgaaatgtc cgttcggttg
     1921 gcagaagcta tgaaacgata tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa
     1981 aactctcttc aattctttat gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg
     2041 cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct
     2101 accgtagtgt ttgtttccaa aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta
     2161 ccaataatcc agaaaattat tatcatggat tctaaaacgg attaccaggg atttcagtcg
     2221 atgtacacgt tcgtcacatc tcatctacct cccggtttta atgaatacga ttttgtacca
     2281 gagtcctttg atcgtgacaa aacaattgca ctgataatga attcctctgg atctactggg
     2341 ttacctaagg gtgtggccct tccgcataga actgcctgcg tcagattctc gcatgccaga
     2401 gatcctattt ttggcaatca aatcattccg gatactgcga ttttaagtgt tgttccattc
     2461 catcacggtt ttggaatgtt tactacactc ggatatttga tatgtggatt tcgagtcgtc
     2521 ttaatgtata gatttgaaga agagctgttt ttacgatccc ttcaggatta caaaattcaa
     2581 agtgcgttgc tagtaccaac cctattttca ttcttcgcca aaagcactct gattgacaaa
     2641 tacgatttat ctaatttaca cgaaattgct tctgggggcg cacctctttc gaaagaagtc
     2701 ggggaagcgg ttgcaaaacg cttccatctt ccagggatac gacaaggata tgggctcact
     2761 gagactacat cagctattct gattacaccc gagggggatg ataaaccggg cgcggtcggt
     2821 aaagttgttc cattttttga agcgaaggtt gtggatctgg ataccgggaa aacgctgggc
     2881 gttaatcaga gaggcgaatt atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac
     2941 aatccggaag cgaccaacgc cttgattgac aaggatggat ggctacattc tggagacata
     3001 gcttactggg acgaagacga acacttcttc atagttgacc gcttgaagtc tttaattaaa
     3061 tacaaaggat atcaggtggc ccccgctgaa ttggaatcga tattgttaca acaccccaac
     3121 atcttcgacg cgggcgtggc aggtcttccc gacgatgacg ccggtgaact tcccgccgcc
     3181 gttgttgttt tggagcacgg aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc
     3241 agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg
     3301 aaaggtctta ccggaaaact cgacgcaaga aaaatcagag agatcctcat aaaggccaag
     3361 aagggcggaa agtccaaatt gtaacacgtg taattctaga gcggccgctt cgagcagaca
     3421 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
     3481 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
     3541 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
     3601 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataagttt aaacagatcc
     3661 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     3721 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     3781 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     3841 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     3901 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     3961 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     4021 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     4081 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     4141 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     4201 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     4261 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     4321 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     4381 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     4441 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     4501 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     4561 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     4621 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     4681 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     4741 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     4801 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     4861 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     4921 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     4981 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     5041 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     5101 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     5161 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     5221 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     5281 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     5341 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     5401 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     5461 tgctcacatg gctcgac
//
