LOCUS       40924_26809        5094 bp DNA     circular SYN 13-JAN-2022
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5094)
  AUTHORS   caoheibi
  TITLE     Direct Submission
REFERENCE   2  (bases 1 to 5094)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..5094
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      12..98
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     CDS             269..925
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     terminator      1363..1449
                     /gene="Escherichia coli rrnB"
                     /label=Escherichia coli rrnB terminator
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      1541..1568
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     terminator      complement(1864..1907)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB
                     gene"
     terminator      2039..2066
                     /note="rrnB T2 terminator"
                     /note="transcription terminator T2 from the E. coli rrnB
                     gene"
     CDS             2648..2836
                     /label=rop
                     /note="Rop protein, which maintains plasmids at low copy
                     number"
     misc_feature    2941..3081
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(3267..3855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(4029..4886)
                     /label=AmpR
                     /note="beta-lactamase"
     promoter        complement(4887..4991)
                     /label=AmpR promoter
ORIGIN
        1 ttcccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
       61 gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgggagcg gatttgaacg
      121 ttgcgaagca acggcccgga gggtggcggg caggacgccc gccataaact gccagggaat
      181 tcccggggat ccgtcgacct gcagccaagc ttgagtagga caaatccgcc gagcttcgac
      241 gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat ataccaccgt
      301 tgatatatcc caatcgcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
      361 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
      421 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
      481 ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
      541 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
      601 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
      661 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
      721 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
      781 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
      841 tcatcatgcc gtctgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
      901 ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac
      961 gcctggtgct acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgtcgagg
     1021 cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga
     1081 actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
     1141 agccgcacgc ggcgcatctc ggctgttttg gcggatgaga gaagattttc agcctgatac
     1201 agattaaatc agaacgcaga agcggtctga taaaacagaa tttgcctggc ggcagtagcg
     1261 cggtggtccc acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta
     1321 gtgtggggtc tccccatgcg agagtaggga actgccaggc atcaaataaa acgaaaggct
     1381 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
     1441 aggacaaatc cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg
     1501 gcaggacgcc cgccataaac tgccaggcat caaattaagc agaaggccat cctgacggat
     1561 ggcctttttg cgtttctaca aactcttcct gtcgtcatat ctacaagcca tccccccaca
     1621 gatacggtaa actagcctcg tttttgcatc aggaaagcag ctgttttggc ggatgagaga
     1681 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     1741 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     1801 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     1861 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1921 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
     1981 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     2041 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttcctgt cgtcatatct
     2101 acaagccatc cccccacaga tacggtaaac tagcctcgtt tttgcatcag gaaagcagtc
     2161 gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg
     2221 gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac
     2281 gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg
     2341 tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg
     2401 gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa
     2461 gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg
     2521 tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag
     2581 catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga
     2641 ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca
     2701 gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg
     2761 tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga
     2821 tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc
     2881 ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg
     2941 cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca
     3001 tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     3061 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     3121 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     3181 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     3241 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     3301 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     3361 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     3421 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     3481 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     3541 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     3601 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     3661 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     3721 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     3781 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     3841 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     3901 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     3961 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4021 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     4081 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     4141 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     4201 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     4261 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     4321 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     4381 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     4441 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     4501 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     4561 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     4621 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa
     4681 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     4741 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     4801 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     4861 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     4921 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     4981 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     5041 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//
