LOCUS       40924_26889        6039 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Transposon vector pkM225, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6039)
  AUTHORS   Mertens K, Berg JP, Brennan RE, Weber MM, Chen C, Samuel JE.
  TITLE     Evolutionary Loss of katE as Balance Mechanism for Antioxidant Gene 
            Expression in Coxiella burnetii
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6039)
  AUTHORS   Mertens K, Berg JP, Brennan RE, Weber MM, Chen C, Samuel JE.
  TITLE     Direct Submission
  JOURNAL   Submitted (09-OCT-2010) Microbial and Molecular Pathogenesis, Texas 
            A
REFERENCE   3  (bases 1 to 6039)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 6039)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (09-OCT-2010) Microbial and Molecular Pathogenesis, Texas A"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..6039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             329..1375
                     /codon_start=1
                     /product="transposase TnA7"
                     /label=transposase TnA7
                     /protein_id="ADQ57456.1"
                     /translation="MEKKEFRVLIKYCFLKGKNTVEAKTWLDNEFPDSAPGKSTIIDWY
                     AKFKRGEMSTEDGERSGRPKEVVTDENIKKIHKMILNDRKMKLIEIAEALKISKERVGH
                     IIHQYLDMRKLCAKWVPRELTFDQKQQRVDDSERCLQLLTRNTPEFFRRYVTMDETWLH
                     HYTPESNRQSAEWTATGEPSPKRGKTQKSAGKVMASVFWDAHGIIFIDYLEKGKTINSD
                     YYMALLERLKVEIAAKRPHMKKKKVLFHQDNAPCHKSLRTMAKIRELGFELLPHPPYSP
                     DLAPSDFFLFSDLKRMLAGKKFGCNEEVIAETEAYFEAKPKEYYRNGIKKLEGRYNRCI
                     ALEGNYVE"
     CDS             1749..2555
                     /label=KanR
                     /note="aminoglycoside phosphotransferase"
     primer_bind     complement(2630..2646)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   2796..2822
                     /note="IR; inverted repeat"
     rep_origin      complement(2923..3511)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             4313..5020
                     /label=mCherry
                     /note="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
     CDS             5310..5966
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
     repeat_region   6011..6037
                     /note="IR; inverted repeat"
ORIGIN
        1 gcggccgcca gtgtgatgga tatctgcaga attcgccctt tataaagctt ggagacaaac
       61 gttacaaccc agccctgcaa ttggaacgaa gcaattgggc cgatgatcag gagcatcgat
      121 tgcacaacgc aatgcaatcg atggatgatc gaagtcgaga tattttatat cagcgttggt
      181 taagtgaaaa aaaatcaacg cttcatgaat tagcagcaca atacgctgtt tctgccgaac
      241 gtattcggca gcttgaaaag aaacgccatg cagaaaattc gaactgctat ggcagatgaa
      301 taatttttaa ctctaagggg aatagcatat ggaaaaaaag gaatttcgtg ttttgataaa
      361 atactgtttt ctgaagggaa aaaatacagt ggaagcaaaa acttggcttg ataatgagtt
      421 tccggactct gccccaggga aatcaacaat aattgattgg tatgcaaaat tcaagcgtgg
      481 tgaaatgagc acggaggacg gtgaacgcag cggacgcccg aaagaggtgg ttaccgacga
      541 aaacatcaaa aaaatccaca aaatgatttt gaatgaccgt aaaatgaagt tgatcgagat
      601 agcagaggcc ttaaagatat cgaaggaacg tgttggtcat atcattcatc aatatttgga
      661 tatgcggaag ctctgtgcaa aatgggtgcc gcgcgagctc acatttgacc aaaaacaaca
      721 acgtgttgat gattctgagc ggtgtttgca gctgttaact cgcaatacac ccgagttttt
      781 ccgtcgatat gtgacaatgg atgaaacatg gctccatcac tacactcctg agtccaatcg
      841 acagtcggct gagtggacgg cgaccggtga accgtctccg aagcgtggaa agactcaaaa
      901 gtccgctggc aaagtaatgg cctctgtttt ttgggatgcg catggaataa tttttatcga
      961 ttatcttgag aagggaaaaa ccatcaacag tgactattat atggcgttat tggagcgttt
     1021 gaaggtcgaa atcgcggcaa aacggcccca catgaagaag aaaaaagtgt tgttccacca
     1081 agacaacgca ccgtgccaca agtcattgag aacgatggca aaaattcgtg aattgggctt
     1141 cgaattgctt ccccacccgc cgtattctcc agatctggcc cccagcgact ttttcttgtt
     1201 ctcagacctc aaaaggatgc tcgcagggaa aaaatttggc tgcaatgaag aggtgatcgc
     1261 cgaaactgag gcctattttg aggcaaaacc gaaggagtac taccgaaatg gtatcaaaaa
     1321 attggaaggt cgttataatc gttgtatcgc tcttgaaggg aactatgttg aataaaagct
     1381 ttgtctgtcg cctcttgtgg cttccggagg agttatttat aataaaataa agaattacga
     1441 cttgaaaatt tttaatcaaa aaaataaagt tgaagattat aaaaagaagt acgaagatag
     1501 agagagaaaa gtggaaggtg aacttaatac cttggatttt accgttaagc gcatgaaaga
     1561 tcaagaaaat acattaaagg cttttgattt taatcaaaga aataaataat aataattatt
     1621 tttaatgcta ttgctatttg cgtttctttt tttttaattt ttgtgctatt ctttttaaca
     1681 ggcaaaagga tttgccaaag tcagaaattt aaattttatc atggaggaat gatatatgat
     1741 tatcaatcat gagccatatt caacgggaaa cgtcgaggcc gcgattaaat tccaacatgg
     1801 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     1861 tctatcgctt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     1921 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     1981 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     2041 cgatccccgg aaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     2101 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     2161 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     2221 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     2281 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     2341 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     2401 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     2461 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     2521 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt
     2581 aacactggca gagcattacg ctgacttgac gggacggcgc aagcttggca ctggccgtcg
     2641 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     2701 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     2761 agttgcgcag cctgaatggc gaatggcggc cgctaacagg ttggctgata agtccccggt
     2821 ctcctaagga tgaacttgcc ggggatctag tgcaggaaag aacatgtgag caaaaggcca
     2881 gcaaaagccc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     2941 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     3001 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     3061 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     3121 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     3181 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     3241 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     3301 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     3361 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     3421 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     3481 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     3541 tgacgctcag tggaacgaaa actcacgtta agggattttg gacgcgtaag ccgatctcgg
     3601 cttgaacgaa ttgttaggtg gcggtacttg ggtcgatatc aaagtgcatc acttcttccc
     3661 gtatgcccaa ctttgtatag agagccactg cgggatcgtc accgtaatct gcttgcacgt
     3721 atctagagtc gacgatgata agatcgacag ggcaatgagg gttgattttg gtcggatccc
     3781 ctttcatgcg cgccatcgca tcgcgcatcg cggctaaatc gaccacggcg ggaacgccgg
     3841 taaaatcttg cattaagact cgcgcgggtc gataagcgat ttcgcggtcg gaatgtttat
     3901 ctttcagcca gtgagcgaac gcttcaatat gcgtttgagt gactgtttct ccatcttcat
     3961 gccgtaattg gttttccaat aaaattttga gtgagtacgg aaggcgatga atgtttgaaa
     4021 gacccgcgtc ttccgccgct tttaagctat ggtagtggta ggttttgcca ccagccgtta
     4081 attcacgccg tgtttttaat gaatccgcca tcgttacctc gcaaccaatt ttgcgcttga
     4141 gcttaacaca ttcgaaagtg cgacagaagt cggtcaagcc gcaaccggcc atgttatgac
     4201 gaagaaatta aaaatcgccc ttgaattttc atcacctgcc cttatatgac tagtgattgt
     4261 cgctcatgtt attgagcttg gtaattttac aaataaatgg agagggttac atatggtgag
     4321 caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
     4381 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
     4441 cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
     4501 ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga
     4561 catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa
     4621 cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt
     4681 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
     4741 gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa
     4801 gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa
     4861 gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa
     4921 gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga
     4981 gggccgccac tccaccggcg gcatggacga gctgtacaag taatctagat gcagaagaca
     5041 aacgttacaa cccagccctg caattggaac gaagcaattg ggccgatgat caggagcatc
     5101 gattgcacaa cgcaatgcaa tcgatggatg atcgaagtcg agatatttat atcagcgttg
     5161 gttaagtgaa aaaaaatcaa cgcttcatga attagcagca caatacgctg tttctgccga
     5221 acgtattcgg cagcttgaaa aaaacgccat gcagaaaatt cgaactgcta tggcagatga
     5281 ataattttta actctaaggg gaatagcata tggagaaaaa aatcactgga tataccaccg
     5341 ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat
     5401 gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa
     5461 ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc
     5521 cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt
     5581 gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg
     5641 acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc
     5701 tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc aatccctggg
     5761 tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt
     5821 tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg
     5881 ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt
     5941 actgcgatga gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa
     6001 cgcctagggc agaccgggga cttatcagcc aacctgtta
//
