LOCUS       40924_25431       10731 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector piggyBac_WH, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10731)
  AUTHORS   Thibault ST, Singer MA, Miyazaki WY, Milash B, Dompe NA, Singh CM, 
            Buchholz R, Demsky M, Fawcett R, Francis-Lang HL, Ryner L, Cheung 
            LM, Chong A, Erickson C, Fisher WW, Greer K, Hartouni SR, Howie E, 
            Jakkula L, Joo D, Killpack K, Laufer A, Mazzotta J, Smith RD, 
            Stevens LM, Stuber C, Tan LR, Ventura R, Woo A, Zakrajsek I, Zhao L,
            Chen F, Swimmer C, Kopczynski C, Duyk G, Winberg ML, Margolis J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster 
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
  PUBMED    14981521
REFERENCE   2  (bases 1 to 10731)
  AUTHORS   Dompe NA, Buchholz R, Miyazaki WY, Kopczynski C, Thibault ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, 
            South San Francisco, CA 94083, USA
REFERENCE   3  (bases 1 to 10731)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10731)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Genet."; date: "2004"; volume: "36"; issue: "3"; pages: "283-287"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, South San 
            Francisco, CA 94083, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10731
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(3747..3794)
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     gene            complement(3988..8124)
                     /label=mini-white
                     /note="This modified version of the white gene lacks part
                     of the first intron."
     primer_bind     complement(10337..10353)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattcgag ctcggtaccc ggggatcctc tagagtcgac gctcgcgcga
     2281 cttggtttgc cattctttag cgcgcgtcgc gtcacacagc ttggccacaa tgtggttttt
     2341 gtcaaacgaa gattctatga cgtgtttaaa gtttaggtcg agtaaagcgc aaatcttttt
     2401 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
     2461 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     2521 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
     2581 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
     2641 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
     2701 gttatagata tcgtgactaa tatataataa aatgggtagt tctttagacg atgagcatat
     2761 cctctctgct cttctgcaaa gcgatgacga gcttgttggt gaggattctg acagtgaaat
     2821 atcagatcac gtaagtgaag atgacgtcca gagcgataca gaagaagcgt ttatagatga
     2881 ggtacatgaa gtgcagccaa cgtcaagcgg tagtgaaata ttagacgaac aaaatgttat
     2941 tgaacaacca ggttcttcat tggcttctaa cagaatcttg accttgccac agaggactat
     3001 tagaggtaag aataaacatt gttggtcaac ttcaaagtcc acgaggcgta gccgagtctc
     3061 tgcactgaac attgtcagat ctcgacgtgt tcactttgct tgtttgaatt gaattgtcgc
     3121 tccgtagacg aagcgcctct atttatactc cggcgctctc tcggtaccct cgacccttgc
     3181 cggaggacag tactccgcca gcccggagga cagtactccg ccttgcgtcg gaggacagta
     3241 ctccgacctc tagtgtcgga ggacagtact ccgaccttgc gtcggaggac agtactccga
     3301 cctctagtgt cggaggacag tactccgacc ttgcgtcgga ggacagtact ccgacctcta
     3361 gtgtcggagg acagtactcc gaccttgccg gaggacagta ctccgcagcc cggaggacag
     3421 tactccgcct tgcgtcggag gacagtactc cgacctctag tgtcggagga cagtactccg
     3481 accttgccgg aggacagtac tccgccagcc cggaggacag tactccgcct tggaggccta
     3541 agcttgatga cctcgagaaa gagagagaga agagaagaga gagaacattc gagaaagaga
     3601 gagagaagag aagagagaga acattcgagg gggggctaga gcggccgccc aattcgccct
     3661 tgaagatcta gctcgttaca gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga
     3721 gcgcttttgg ttttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
     3781 ggaataggaa cttcaaagcg tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg
     3841 cgcacataca gctcactgtt cacgtcgcaa gatcttcaag ggcgaattga ggtcctgttc
     3901 attgtttaat gaaaataaga gcttgaggga aaaaattcgt actttggagt acgaaatgcg
     3961 tcgtttagag cagcagccga attaattcta gttccagtga aatccaagca ttttctaaat
     4021 taaatgtatt cttattatta tagttgttat ttttgatata tataaacaac actattatgc
     4081 ccaccatttt tttgagatgc atctacacaa ggaacaaaca ctggatgtca ctttcagttc
     4141 aaattgtaac gctaatcact ccgaacaggt cacaaaaaat taccttaaaa agtcataata
     4201 ttaaattaga ataaatatag ctgtgaggga aatatataca aatatattgg agcaaataaa
     4261 ttgtacatac aaatatttat tactaatttc tattgagacg aaatgaacca ctcggaacca
     4321 tttgagcgaa ccgaatcgcg cggaactaac gacagtcgct ccaaggtcgt cgaacaaaag
     4381 gtgaatgtgt tgcggagagc gggtgggaga cagcgaaaga gcaactacga aacgtggtgt
     4441 ggtggaggtg aattatgaag agggcgcgcg atttgaaaag tatgtatata aaaaatatat
     4501 cccggtgttt tatgtagcga taaacgagtt tttgatgtaa ggtatgcagg tgtgtaagtc
     4561 ttttggttag aagacaaatc caaagtctac ttgtggggat gttcgaaggg gaaatacttg
     4621 tattctatag gtcatatctt gtttttattg gcacaaatat aattacatta gctttttgag
     4681 ggggcaataa acagtaaaca cgatggtaat aatggtaaaa aaaaaaacaa gcagttattt
     4741 cggatatatg tcggctactc cttgcgtcgg gcccgaagtc ttagagccag atatgcgagc
     4801 acccggaagc tcacgatgag aatggccaga cccacgtagt ccagcggcag atcggcggcg
     4861 gagaagttaa gcgtctccag gatgaccttg cccgaactgg ggcacgtggt gttcgacgat
     4921 gtgcagctaa tttcgcccgg ctccacgtcc gcccattggt taatcagcag accctcgttg
     4981 gcgtaacgga accatgagag gtacgacaac catttgaggt atactggcac cgagcccgag
     5041 ttcaagaaga agccgccaaa gagcaggaat ggtatgataa ccggcggacc cacagacagc
     5101 gccatcgagg tcgaggagct ggcgcaggat attagatatc cgaaggacgt tgacacattg
     5161 gccaccagag tgaccagcgc caggcagttg aagaagtgca gcactccggc ccgcagtccg
     5221 atcatcggat aggcaatcgc cgtgaagacc agtggcactg tgagaaaaag cggtaattcg
     5281 gcaatcgttt tgcccagaaa gtatgtgtca cagcgataaa gtcgacttcg ggcctccctc
     5341 ataaaaactg gcagctctga ggtgaacacc taaatcgaat cgattcatta gaaagttagt
     5401 aaattattaa tatgcaaatg tattctaaac aagacttaca tttatcgtgg caaagacgtt
     5461 ttgaaaggtc atgttggtca ggaagaggaa gatggctccg ttgatattca tcacgcccac
     5521 ttgcgtgagt tgttggccca aaaagatgag gccaatcaag atggcaacca tctgcaaatt
     5581 aaaatgttac tcgcatctca ttaatattca tatcttcaac atgttcgcga gttaaatgaa
     5641 atttatttat tttctgcaaa actataaact atacatctca ttgaaaaaaa ctaagaaggg
     5701 tgtggaatca ggcaattcta actaaaatct agcgaatttg tttccaagaa ttgtaagcgt
     5761 tatatcattt gtttccactg gaaccactca ccgttgtctg aataagtcgc acttttacga
     5821 ggagtggttc cttgagcacc gacagccagg atcgccacag gaccgcccgg aactgcatga
     5881 accaggtggc cttgtaggtg tacccattct ccggctgctc cagtggcttc tccaaatttt
     5941 tggtggccaa caactgctcc atatcccggg ctactttgct aatagcaaaa ttgtcgcata
     6001 tcttggcgat ccgatcacgg gactcgatct cccgtccggg cacaacggcc aacacctgta
     6061 cgtaaaagtc cgccggattg tagttggtag gacactgggc acccacgctg gataggagtt
     6121 gagatgtaat gtaatgctag atacccttaa taaacacatc gaactcacta ggaaaagaag
     6181 tcgacggctt cgctgggagt gcccaagaaa gctaccctgc cctcggccat cagaaggatc
     6241 ttgtcaaaga gctcaaacag ctcggaagac ggctgatgaa tggtcaggat gacggtcttg
     6301 cccttctgcg acagcttctt cagcacctgg acgacgctgt gggcggtaaa tgagtccagt
     6361 ccggaggtgg gctcatcgca gatcagaagc ggcggatcgg ttagtgcctc ggaggcgaat
     6421 gccagacgct tcctttctcc gccggacaga cctttcaccc tgccgggcac accgatgatc
     6481 gtgtgctgac atttgctgag cgaaagctcc tggatcacct gatccacgcg ggccactcgc
     6541 tgccgatagg tcagatgtcg tggcatccgc accatggcct ggaaaatcag gtgttccctg
     6601 gccgttaggg agccgataaa gaggtcatcc tgctggacat aggcgcacct ggcctgcatc
     6661 tccttggcgt ccacaggttg gccattgagc agtcgcatcc cggatggcga tacttggatg
     6721 ccctgcggcg atcgaaaggc aagggcattc agcagggtcg tctttccggc accggaactg
     6781 cccatcacgg ccaaaagttc gcccggatag gccacgccgc aaactgagtt tcaaattggt
     6841 aattggaccc tttattaaga tttcacacag atcagccgac tgcgaataga aactcaccgt
     6901 tcttgagcaa atgtttcctg ggcgccggta tgtgtcgctc gttgcagaat agtccgcgtg
     6961 tccggttgac cagctgccgc catccggagc ccggctgatt gaccgcccca aagatgtcca
     7021 tattgtgcca ggcataggtg aggttctcgg ctagttggcc gctccctgaa ccggagtcct
     7081 ccggcggact gggtggcagg agcgtgccgt agtttttggc ctgcccgaag ccctggttaa
     7141 tgcagctctg cgaagccgct ccgctgtcac cctgcaatga taggggatct caaatatcaa
     7201 ctacaagcgt tatgctcatc taaccccgaa caaaacgaag tatcctacga agtaggttta
     7261 tacttttatt tattttttgt gcatctagga tcagcttaaa atatctggtt gttatatttt
     7321 ttgtaaaaaa gaatgtagtc gaaaatgaat gcctttagat gtcttgatca tgatatgatc
     7381 ttaaaaattg tcttatatag cgagcacagc taccagaata atctgtttcg tgtcactatt
     7441 tgtttgtgcg attgcggttt gggatttttg tgggtcgcag ttctcacgcc gcagacaatt
     7501 tgatgttgca atcgcagttc ctatagatca agtgaactta agatgtatgc acatgtacta
     7561 ctcacattgt tcagatgctc ggcagatggg tgtttgctgc ctccgcgaat taatagctcc
     7621 tgatcctctt ggcccattgc cgggattttt cacactttcc cctgcttacc cacccaaaac
     7681 caatcaccac cccaatcact caaaaaacaa acaaaaataa gaagcgagag gagttttggc
     7741 acagcacttt gtgtttaatt gatggcgtaa accgcttgga gcttcgtcac gaaaccgctg
     7801 acaaagtgca actgaaggcg gacattgacg ctaggtaacg ctacaaacgg tggcgaaaga
     7861 gatagcggac gcagcggcga aagagacggc gatatttctg tggacagaga aggaggcaaa
     7921 cagcgctgac tttgagtgga atgtcatttt gagtgagagg taatcgaaag aacctggtac
     7981 ttcaaatacc cttggatcga agtaaattta aaactgatca gataagttca atgatgtcca
     8041 gtgcagtaaa aaataaaaaa aaaatatgtt tttttaaatc tacattctcc aaaaaagggt
     8101 tttattaact tacatacata ctagaattcg gcttgggtcg accgctaggt aacgctacaa
     8161 acggtggcga aagagatagc ggacgcagcg gcgaaagaga cggcgatatt tctgtggaca
     8221 gagaaggagg caaacagcgc tgactttgag tggaatgtca ttttgagtga gaggtaatcg
     8281 aaagaacctg gtacatcaaa tacccttgga tcgaagtaaa tttaaaactg atcagataag
     8341 ttcaatgata tccagtgcag taaaaaaaaa aaatgttttt tttatctact ttccgcaaaa
     8401 atgggtttta ttaacttaca tacatactag aattaattcc tcgactatgg caagaaaaag
     8461 tatgcaatat aataatcttt tattgggtat gcaacgaaaa tttgtttcgt caaagtatgc
     8521 aatatttttt attaaaagag ggtatgcaat gtattttatt aaaaacgggt atgcaataca
     8581 ataatctttt attgggtast gcaacgaaaa tttgtttcgt caaagtatgc aatatttttt
     8641 attaaaagag ggtatgcaat gtattttatt aaaaacgggt atgcaataaa aaattatttg
     8701 gtttctctaa aaagtatgca gcacttattt tttgataagg tatgcgacaa aattttactt
     8761 tgccgaaaat atgcaatgtt tttgcgaata aattgtcgag cccagtaagc cgaattcggt
     8821 acccgcccgg gatcagatag atctccgcgg ccgccttaag ctgcagtagg aagacgaata
     8881 ggtggcctat ggcattattg tacggaatga taaacattgc ctgcataaat tcttttatta
     8941 tatacagcca taatgtcagt agcaagggag aaaaggtcca aagtcgcaaa aaatttatga
     9001 gaaaccttta catgagcctg acgtcatcgt ttatgcgtaa gcgtttagaa gctcctactt
     9061 tgaagagata tttgcgcgat aatatctcta atattttgcc aaatgaagtg cctggtacat
     9121 cagatgacag tactgaagag ccagtaatga aaaaacgtac ttactgtact tactgcccct
     9181 ctaaaataag gcgaaaggca aatgcatcgt gcaaaaaatg caaaaaagtt atttgtcgag
     9241 agcataatat tgatatgtgc caaagttgtt tctgactgac taataagtat aatttgtttc
     9301 tattatgtat aagttaagct aattacttat tttataatac aacatgactg tttttaaagt
     9361 acaaaataag tttatttttg taaaagagag aatgtttaaa agttttgtta ctttatagaa
     9421 gaaattttga gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg
     9481 aatttattat tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata
     9541 aataaacctc gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt
     9601 taacgtacgt cacaatatga ttatctttct agggttaaat aatagtttct aattttttta
     9661 ttattcagcc tgctgtcgtg aataccgtat atctcaacgc tgtctgtgag attgtcgtat
     9721 tctagccttt ttagtttttc gctcatcgac ttgatattgt ccgacacatt ttcgtcgatt
     9781 tgcgttttga tcaaagactt gagcagagac acgttaatca actgttcaaa ttgatccata
     9841 ttaacgatat caacccgatg cgtatatggt gcgtaaaata tattttttaa ccctcttata
     9901 ctttgcactc tgcgttaata cgcgttcgtg tacagacgta atcatgtttt cttttttgga
     9961 taaaactcct actgagtttg acctcatatt agaccctcac aagttgcaaa acgtggcatt
    10021 ttttaccaat gaagaattta aagttatttt aaaaaatttc atcacagatt taaagaagaa
    10081 ccaaaaatta aattatttca acagtttaat cgaccagtta atcaacgtgt acacagacgc
    10141 gtcggcaaaa aacacgcagc ccgacgtgtt ggctaaaatt attaaatcaa cttgtgttat
    10201 agtcacggat ttgccgtcca acgtgttcct caaaaagttg aagaccaaca agtttacgga
    10261 cactattaat tatttgattt tgccccactt cattttgtgg gatcacaatt ttgttatatt
    10321 ttaaacaaag cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
    10381 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
    10441 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
    10501 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca
    10561 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
    10621 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
    10681 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
