LOCUS       40924_25421       10781 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector piggyBac_PB, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Thibault ST, Singer MA, Miyazaki WY, Milash B, Dompe NA, Singh CM, 
            Buchholz R, Demsky M, Fawcett R, Francis-Lang HL, Ryner L, Cheung 
            LM, Chong A, Erickson C, Fisher WW, Greer K, Hartouni SR, Howie E, 
            Jakkula L, Joo D, Killpack K, Laufer A, Mazzotta J, Smith RD, 
            Stevens LM, Stuber C, Tan LR, Ventura R, Woo A, Zakrajsek I, Zhao L,
            Chen F, Swimmer C, Kopczynski C, Duyk G, Winberg ML, Margolis J.
  TITLE     A complementary transposon tool kit for Drosophila melanogaster 
            using P and piggyBac
  JOURNAL   Nat. Genet. 36 (3), 283-287 (2004)
  PUBMED    14981521
REFERENCE   2  (bases 1 to 10781)
  AUTHORS   Thibault ST.
  TITLE     Direct Submission
  JOURNAL   Submitted (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, 
            South San Francisco, CA 94083, USA
REFERENCE   3  (bases 1 to 10781)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 10781)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat. 
            Genet."; date: "2004"; volume: "36"; issue: "3"; pages: "283-287"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (19-DEC-2003) Exelixis, Inc., 170 Harbor Way, PO Box 511, South San 
            Francisco, CA 94083, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..10781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        96..200
                     /label=AmpR promoter
     CDS             201..1058
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1232..1820
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2108..2129
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        2144..2174
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2182..2198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2206..2222
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    4498..4545
                     /label=FRT
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             complement(join(5211..5825,5896..6027,6231..6576,6609..7263,
                     7338..7638,7996..8067))
                     /codon_start=1
                     /gene="white"
                     /allele="+mC"
                     /product="white protein"
                     /label=white
                     /protein_id="AAS09817.1"
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNLIFEIPYHCRVTADASQSCIN
                     QGFGQAKNYGTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRT
                     RGLFCNERHIPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVS
                     PSGMRLLNGQPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVA
                     RVDQVIQELSLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLD
                     SFTAHSVVQVLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDF
                     FSYITLHLNSYPAWVPSVLPTTIRRTFTYRCWPLCPDGRSSPVIGSPRYGDNFAISKVA
                     RDMEQLLATKNLEKPLEQPENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTT
                     MVAILIGLIFLGQQLTQVGVMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARS
                     RLYRCDTYFLGKTIAELPLFLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVST
                     SFGYLISCASSSTSMALSVGPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEG
                     LLINQWADVEPGEISCTSSNTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLA
                     YLALRLRARRKE"
     gene            complement(5211..8067)
                     /gene="white"
                     /allele="+mC"
                     /label=white
     protein_bind    8566..8613
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     primer_bind     complement(10387..10403)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
       61 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      121 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
      181 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
      241 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
      301 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
      361 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
      421 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
      481 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
      541 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
      601 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
      661 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
      721 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
      781 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
      841 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
      901 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
      961 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     1021 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     1081 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     1141 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     1201 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     1261 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     1321 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     1381 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     1441 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     1501 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     1561 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     1621 agcattgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     1681 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     1741 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     1801 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     1861 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     1921 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     1981 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     2041 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     2101 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     2161 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     2221 accatgatta cgaattcgag ctcggtaccc ggggatcctc tagagtcgac gctcgcgcga
     2281 cttggtttgc cattctttag cgcgcgtcgc gtcacacagc ttggccacaa tgtggttttt
     2341 gtcaaacgaa gattctatga cgtgtttaaa gtttaggtcg agtaaagcgc aaatcttttt
     2401 taaccctaga aagatagtct gcgtaaaatt gacgcatgca ttcttgaaat attgctctct
     2461 ctttctaaat agcgcgaatc cgtcgctgtg catttaggac atctcagtcg ccgcttggag
     2521 ctcccgtgag gcgtgcttgt caatgcggta agtgtcactg attttgaact ataacgaccg
     2581 cgtgagtcaa aatgacgcat gattatcttt tacgtgactt ttaagattta actcatacga
     2641 taattatatt gttatttcat gttctactta cgtgataact tattatatat atattttctt
     2701 gttatagata tcgtgactaa tatataataa aatgggtagt tctttagacg atgagcatat
     2761 cctctctgct cttctgcaaa gcgatgacga gcttgttggt gaggattctg acagtgaaat
     2821 atcagatcac gtaagtgaag atgacgtcca gagcgataca gaagaagcgt ttatagatga
     2881 ggtacatgaa gtgcagccaa cgtcaagcgg tagtgaaata ttagacgaac aaaatgttat
     2941 tgaacaacca ggttcttcat tggcttctaa cagaatcttg accttgccac agaggactat
     3001 tagaggtaag aataaacatt gttggtcaac ttcaaagtcc acgaggcgta gccgagtctc
     3061 tgcactgaac attgtcagat ctcaaagagg tccgacgcgt atgtgccgca atatatatga
     3121 cccactttta tgcttcaaac tattttttac tgatgagata atttcggaaa ttgtaaaatg
     3181 gacaaatgct gagatatcat tgaaacgtcg ggaatctatg acaggtgcta catttcgtga
     3241 cacgaatgaa gatgaaatct atgctttctt tggtattctg gtaatgacag cagtgagaaa
     3301 agataaccac atgtccacag atgacctctt tgatcgatct ttgtcaatgg tgtacgtctc
     3361 tgtaatgagt cgtgatcgtt ttgatttttt gatacgatgt cttagaatgg atgacaaaag
     3421 tatacggccc acacttcgag aaaacgatgt atttactcct gttagaaaaa tatgggatct
     3481 ctttatccat cagtgcatac aaaattacac tccaggggct catttgacca tagatgaaca
     3541 gttacttggt tttagaggac ggtgtccgtt taggatgtat atcccaaaca agccaagtaa
     3601 gtatggaata aaaatcctca tgatgtgtga cagtggtacg aagtatatga taaatggaat
     3661 gccttatttg ggaagaggaa cacagaccaa cggagtacca ctcggtgaat actacgtgaa
     3721 ggagttatca aagcctgtgc acggtagttg tcgtaatatt acgtgtgaca attggttcac
     3781 ctcaatccct ttggcaaaaa acttactaca agaaccgtat aagttagctt accgaagtat
     3841 acacttaaat tcagtgcacg tttgcttgtt gagaggaaag gttgtgtgcg gacgaatttt
     3901 tttttgaaaa cattaaccct tacgtggaat aaaaaaaaat gaaatattgc aaattttgct
     3961 gcaaagctgt gactggagta aaattaattc acgtgccgaa gtgtgctatt aagagaaaat
     4021 tgtgggagca gagccttggg tgcagccttg gtgaaaactc ccaaatttgt gatacccact
     4081 ttaatgattc gcagtggaag gctgcacctg caaaaggtca gacatttaaa aggaggcgac
     4141 tcaacgcaga tgccgtacct agtaaagtga tagagcctga accagaaaag ataaaagaag
     4201 gctataccag tgggagtaca caaacagagt aagtttgaat agtaaaaaaa atcatttatg
     4261 taaacaataa cgtgactgtg cgttaggtcc tgttcattgt ttaatgaaaa taagagcttg
     4321 agggaaaaaa ttcgtacttt ggagtacgaa atgcgtcgtt tagagcagca gccgaattaa
     4381 ttctagttcc agtgaaatcc aagcattttc taaattaaat gtattcttat tattatagtt
     4441 gttatttttg atatatataa acaacactat tatgcccacc atttttttga gatgcatgaa
     4501 gttcctattc cgaagttcct attctctaga aagtatagga acttcgctag cgtgcatcta
     4561 cacaaggaac aaacactgga tgtcactttc agttcaaatt gtaacgctaa tcactccgaa
     4621 caggtcacaa aaaattacct taaaaagtca taatattaaa ttagaataaa tatagctgtg
     4681 agggaaatat atacaaatat attggagcaa ataaattgta catacaaata tttattacta
     4741 atttctattg agacgaaatg aaccactcgg aaccatttga gcgaaccgaa tcgcgcggaa
     4801 ctaacgacag tcgctccaag gtcgtcgaac aaaaggtgaa tgtgttgcgg agagcgggtg
     4861 ggagacagcg aaagagcaac tacgaaacgt ggtgtggtgg aggtgaatta tgaagagggc
     4921 gcgcgatttg aaaagtatgt atataaaaaa tatatcccgg tgttttatgt agcgataaac
     4981 gagtttttga tgtaaggtat gcaggtgtgt aagtcttttg gttagaagac aaatccaaag
     5041 tctacttgtg gggatgttcg aaggggaaat acttgtattc tataggtcat atcttgtttt
     5101 tattggcaca aatataatta cattagcttt ttgagggggc aataaacagt aaacacgatg
     5161 gtaataatgg taaaaaaaaa aaacaagcag ttatttcgga tatatgtcgg ctactccttg
     5221 cgtcgggccc gaagtcttag agccagatat gcgagcaccc ggaagctcac gatgagaatg
     5281 gccagaccca cgtagtccag cggcagatcg gcggcggaga agttaagcgt ctccaggatg
     5341 accttgcccg aactggggca cgtggtgttc gacgatgtgc agctaatttc gcccggctcc
     5401 acgtccgccc attggttaat cagcagaccc tcgttggcgt aacggaacca tgagaggtac
     5461 gacaaccatt tgaggtatac tggcaccgag cccgagttca agaagaagcc gccaaagagc
     5521 aggaatggta tgataaccgg cggacccaca gacagcgcca tcgaggtcga ggagctggcg
     5581 caggatatta gatatccgaa ggacgttgac acattggcca ccagagtgac cagcgccagg
     5641 cagttgaaga agtgcagcac tccggcccgc agtccgatca tcggataggc aatcgccgtg
     5701 aagaccagtg gcactgtgag aaaaagcggc aattcggcaa tcgttttgcc cagaaagtat
     5761 gtgtcacagc gataaagtcg acttcgggcc tccctcataa aaactggcag ctctgaggtg
     5821 aacacctaaa tcgaatcgat tcattagaaa gttagtaaat tattgaaatg caaatgtatt
     5881 ctaaacatga cttacattta tcgtggcaaa gacgttttga aaggtcatgt tggtcaggaa
     5941 gaggaagatg gctccgttga tattcatcac acccacttgc gtgagttgtt ggcccaaaaa
     6001 gatgaggcca atcaagatgg caaccatctg caaattaaaa tgttactcgc atctcattaa
     6061 tattcgcgag ttaaatgaaa tttatttatc ttctgcaaaa ctataaacta tacatctcat
     6121 tgaaaaaaac taagaagggt gtggaatcag gcaattctat ctaaaatcta gcgaatttgt
     6181 ttccaagaat tgtaagcgtt atatcatttg tttccactgg aaccactcac cgttgtctga
     6241 ataagtcgca cttttacgag gagtggttcc ttgagcaccg acagccagga tcgccacagg
     6301 accgcccgga actgcatgaa ccaggtggcc ttgtaggtgt acccattctc cggctgctcc
     6361 agtggcttct ccagattttt ggtggccaac aactgctcca tatcccgggc tactttgcta
     6421 atggcaaaat tgtcgccata tcttggcgat ccgatcacgg gactcgatct cccgtccggg
     6481 cacaacggcc aacacctgta cgtaaaagtc cgccggattg tagttggtag gacactgggc
     6541 acccacgctg gataggagtt gagatgtaat gtaatgctag atacccttaa taaacacatc
     6601 gaactcacta ggaaaagaag tcgacggctt cgctgggagt gcccaagaaa gctaccctgc
     6661 cctcggccat cagaaggatc ttgtcaaaga gctcaaacag ctcggaagac ggctgatgaa
     6721 tggtcaggat gacggtcttg cccttctgcg acagcttctt cagcacctgg acgacgctgt
     6781 gggcggtaaa tgagtccagt ccggaggtgg gctcatcgca gatcagaagc ggcggatcgg
     6841 ttagtgcctc ggaggcgaat gccagacgct tcctttctcc gccggacaga cctttcaccc
     6901 tgccgggcac accgatgatc gtgtgctgac atttgctgag cgaaagctcc tggatcacct
     6961 gatccacgcg ggccactcgc tgccgatagg tcagatgtcg tggcatccgc accatggctt
     7021 ggaaaatcag gtgttccctg gccgttaggg agccgataaa gaggtcatcc tgctggacat
     7081 aggcgcacct ggcctgcatc tccttggcgt ccacaggttg gccattgagc agtcgcatcc
     7141 cggatggcga tacttggatg ccctgcggcg atcgaaaggc aagggcattc agcagggtcg
     7201 tctttccggc accggaactg cccatcacgg ccaaaagttc gcccggatag gccacgccgc
     7261 aaactgagtt tcaaattggt aattggaccc tttattaaga tttcacacag atcagccgac
     7321 tgcgaataga aactcaccgt tcttgagcaa atgtttcctg ggcgccggta tgtgtcgctc
     7381 gttgcagaat agtccgcgtg tccggttgac cagctgccgc catccggagc ccggctgatt
     7441 gaccgcccca aagatgtcca tattgtgcca ggcataggtg aggttctcgg ctagttggcc
     7501 gctccctgaa ccggagtcct ccggcggact gggtggcagg agcgtgccgt agtttttggc
     7561 ctgcccgaag ccctggttaa tgcagctctg cgaagcgtcc gctgtcaccc tgcaatgata
     7621 ggggatctca aatatcaact acaagcgtta tgctcatcta accccgaaca aaacgaagta
     7681 tcctacgaag taggtttata cttttattta ttttttgtgc atagcttaaa atatctggtt
     7741 gttatatttt ttgtaaaaaa gaatgtagtc gaaaatgaat gcctttagat gtcttgatca
     7801 tgatatgatc ttaaaaattg tcttatatag cgagcacagc taccagaata atctgtttcg
     7861 tgtcactatt tgtttgtgcg attgcggttt gggatttttg tgggtcgcag ttctcacgcc
     7921 gcagacaatt tgatgttgca atcgcagttc ctatagatca agtgaactta agatgtatgc
     7981 acatgtacta ctcacattgt tcagatgctc ggcagatggg tgtttgctgc ctccgcgaat
     8041 taatagctcc tgatcctctt ggcccattgc cgggattttt cacactttcc cctgcttacc
     8101 cacccaaaac caatcaccac cccaatcact caaaaaacaa acaaaaataa gaagcgagag
     8161 gagttttggc acagcacttt gtgtttaatt gatggcgtaa accgcttgga gcttcgtcac
     8221 gaaaccgctg acaaagtgca actgaaggcg gacattgacg ctaggtaacg ctacaaacgg
     8281 tggcgaaaga gatagcggac gcagcggcga aagagacggc gatatttctg tggacagaga
     8341 aggaggcaaa cagcgctgac tttgagtgga atgtcatttt gagtgagagg taatcgaaag
     8401 aacctggtac atcaaatacc cttggatcga agtaaattta aaactgatca gataagttca
     8461 atgatatcca gtgcagtaaa aaaaaaaaat gtttttttta tctactttcc gcaaaaatgg
     8521 gttttattaa cttacataca tactagaatt aattcctcga ctagtgaagt tcctattccg
     8581 aagttcctat tctctagaaa gtataggaac ttcgctagtg cctgcagcca agctaaccat
     8641 tgtgggaacc gtgcgatcaa acaaacgcga gataccggaa gtactgaaaa acagtcgctc
     8701 caggccagtg ggaacatcga tgttttgttt tgacggaccc cttactctcg tctcatataa
     8761 accgaagcca gctaagatgg tatacttatt atcatcttgt gatgaggatg cttctatcaa
     8821 cgaaagtacc ggtaaaccgc aaatggttat gtattataat caaactaaag gcggagtgga
     8881 cacgctagac caaatgtgtt ctgtgatgac ctgcagtagg aagacgaata ggtggcctat
     8941 ggcattattg tacggaatga taaacattgc ctgcataaat tcttttatta tatacagcca
     9001 taatgtcagt agcaagggag aaaaggtcca aagtcgcaaa aaatttatga gaaaccttta
     9061 catgagcctg acgtcatcgt ttatgcgtaa gcgtttagaa gctcctactt tgaagagata
     9121 tttgcgcgat aatatctcta atattttgcc aaatgaagtg cctggtacat cagatgacag
     9181 tactgaagag ccagtaatga aaaaacgtac ttactgtact tactgcccct ctaaaataag
     9241 gcgaaaggca aatgcatcgt gcaaaaaatg caaaaaagtt atttgtcgag agcataatat
     9301 tgatatgtgc caaagttgtt tctgactgac taataagtat aatttgtttc tattatgtat
     9361 aagttaagct aattacttat tttataatac aacatgactg tttttaaagt acaaaataag
     9421 tttatttttg taaaagagag aatgtttaaa agttttgtta ctttatagaa gaaattttga
     9481 gtttttgttt ttttttaata aataaataaa cataaataaa ttgtttgttg aatttattat
     9541 tagtatgtaa gtgtaaatat aataaaactt aatatctatt caaattaata aataaacctc
     9601 gatatacaga ccgataaaac acatgcgtca attttacgca tgattatctt taacgtacgt
     9661 cacaatatga ttatctttct agggttaaat aatagtttct aattttttta ttattcagcc
     9721 tgctgtcgtg aataccgtat atctcaacgc tgtctgtgag attgtcgtat tctagccttt
     9781 ttagtttttc gctcatcgac ttgatattgt ccgacacatt ttcgtcgatt tgcgttttga
     9841 tcaaagactt gagcagagac acgttaatca actgttcaaa ttgatccata ttaacgatat
     9901 caacccgatg cgtatatggt gcgtaaaata tattttttaa ccctcttata ctttgcactc
     9961 tgcgttaata cgcgttcgtg tacagacgta atcatgtttt cttttttgga taaaactcct
    10021 actgagtttg acctcatatt agaccctcac aagttgcaaa acgtggcatt ttttaccaat
    10081 gaagaattta aagttatttt aaaaaatttc atcacagatt taaagaagaa ccaaaaatta
    10141 aattatttca acagtttaat cgaccagtta atcaacgtgt acacagacgc gtcggcaaaa
    10201 aacacgcagc ccgacgtgtt ggctaaaatt attaaatcaa cttgtgttat agtcacggat
    10261 ttgccgtcca acgtgttcct caaaaagttg aagaccaaca agtttacgga cactattaat
    10321 tatttgattt tgccccactt cattttgtgg gatcacaatt ttgttatatt ttaaacaaag
    10381 cttggcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
    10441 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
    10501 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt
    10561 tctccttacg catctgtgcg gtatttcaca ccgcatatgg tgcactctca gtacaatctg
    10621 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
    10681 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
    10741 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg a
//
