LOCUS       V000457                 8502 bp    DNA     circular SYN 13-MAY-2021
DEFINITION  Exported.
ACCESSION   V000457
VERSION     V000457
KEYWORDS    phage-cmv-cfp-24xms2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 8502)
  AUTHORS   Wu B, Chao JA, Singer RH
  TITLE     Fluorescence fluctuation spectroscopy enables quantitative imaging
            of single mRNAs in living cells.
  JOURNAL   Biophys J. 2012 Jun 20;102(12):2936-44. Epub 2012 Jun 19.
   PUBMED   22735544
REFERENCE   2  (bases 1 to 8502)
  TITLE     Direct Submission
REFERENCE   3  (bases 1 to 8502)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biophys
            J."; date: "2012-06-20"; volume: "102(12)"; pages: "2936-44. Epub
            2012 Jun 19"
            SGRef: number: 2; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..8502
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label="3' LTR"
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label="HIV-1 Psi"
                     /note="packaging signal of human immunodeficiency virus
                     type 1"
     misc_feature    1303..1536
                     /label="RRE"
                     /note="The Rev response element (RRE) of HIV-1 allows for
                     Rev-dependent mRNA export from the nucleus to the
                     cytoplasm."
     CDS             1721..1765
                     /label="gp41 peptide"
                     /note="antigenic peptide corresponding to amino acids 655
                     to 669 of the HIV envelope protein gp41 (Lutje Hulsik et
                     al., 2013)"
     CDS             1914..1955
                     /note="Protein Tat from Human immunodeficiency virus type 1
                     group M subtype B (isolate WMJ22). Accession#: P12509"
                     /label="Protein Tat"
     misc_feature    2058..2175
                     /label="cPPT/CTS"
                     /note="central polypurine tract and central termination
                     sequence of HIV-1"
     enhancer        2227..2530
                     /label="CMV enhancer"
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2531..2734
                     /label="CMV promoter"
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     primer_bind     2731..2755
                     /label="LNCX"
                     /note="Human CMV promoter, forward primer"
     CDS             2827..3543
                     /label="ECFP"
                     /note="enhanced CFP"
     misc_RNA        3583..3601
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        3622..3640
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        3692..3710
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        3731..3749
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        3801..3819
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        3840..3858
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        3910..3928
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        3949..3967
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4019..4037
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4058..4076
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4128..4146
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4167..4185
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4237..4255
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4276..4294
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4346..4364
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4457..4475
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4496..4514
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4566..4584
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4605..4623
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4675..4693
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4714..4732
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4784..4802
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_RNA        4824..4842
                     /label="MS2 stem loop"
                     /note="stem loop that binds the bacteriophage MS2 coat
                     protein"
     misc_feature    4892..5480
                     /label="WPRE"
                     /note="woodchuck hepatitis virus posttranscriptional
                     regulatory element"
     LTR             5555..5788
                     /label="3' LTR (Delta-U3)"
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5907..5925)
                     /label="pBRforEco"
                     /note="pBR322 vectors, upsteam of EcoRI site, forward
                     primer"
     promoter        5993..6097
                     /label="AmpR promoter"
     CDS             6098..6955
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      7129..7717
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     primer_bind     7871..7888
                     /label="L4440"
                     /note="L4440 vector, forward primer"
     primer_bind     7980..7999
                     /label="SV40pro-F"
                     /note="SV40 promoter/origin, forward primer"
     protein_bind    8128..8149
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        8164..8194
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    8202..8218
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8226..8242
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     primer_bind     8292..8311
                     /label="EBV-rev"
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
     2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
     2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
     2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
     2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc accaccggtc
     2821 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2881 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     2941 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3001 cccaccctcg tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac
     3061 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3121 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3181 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3241 gggcacaagc tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag
     3301 aagaacggca tcaaggccaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3361 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3421 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3481 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3541 aagtaaagcg gccggatcct aaggtaccta attgcctaga aaacatgagg atcacccatg
     3601 tctgcaggtc gactctagaa aacatgagga tcacccatgt ctgcagtatt cccgggttca
     3661 ttagatccta aggtacctaa ttgcctagaa aacatgagga tcacccatgt ctgcaggtcg
     3721 actctagaaa acatgaggat cacccatgtc tgcagtattc ccgggttcat tagatcctaa
     3781 ggtacctaat tgcctagaaa acatgaggat cacccatgtc tgcaggtcga ctccagaaaa
     3841 catgaggatc acccatgtct gcagtattcc cgggttcatt agatcctaag gtacctaatt
     3901 gcctagaaaa catgaggatc acccatgtct gcaggtcgac tctagaaaac atgaggatca
     3961 cccatgtctg cagtattccc gggttcatta gatcctaagg tacctaattg cctagaaaac
     4021 atgaggatca cccatgtctg caggtcgact ctagaaaaca tgaggatcac ccatgtctgc
     4081 agtattcccg ggttcattag atcctaaggt acctaattgc ctagaaaaca tgaggatcac
     4141 ccatgtctgc aggtcgactc cagaaaacat gaggatcacc catgtctgca gtattcccgg
     4201 gttcattaga tcctaaggta cctaattgcc tagaaaacat gaggatcacc catgtctgca
     4261 ggtcgactct agaaaacatg aggatcaccc atgtctgcag tattcccggg ttcattagat
     4321 cctaaggtac ctaattgcct agaaaacatg aggatcaccc atgtctgcag gtcgactcta
     4381 gaaaacatga gaggatcacc catgtctgca gtattcccgg gttcattaga tcctaaggta
     4441 cctaattgcc tagaaaacat gaggatcacc catgtctgca ggtcgactct agaaaacatg
     4501 aggatcaccc atgtctgcag tattcccggg ttcattagat cctaaggtac ctaattgcct
     4561 agaaaacatg aggatcaccc atgtctgcag gtcgactcta gaaaacatga ggatcaccca
     4621 tgtctgcagt attcccgggt tcattagatc ctaaggtacc taattgccta gaaaacatga
     4681 ggatcaccca tgtctgcagg tcgactctag aaaacatgag gatcacccat gtctgcagta
     4741 ttcccgggtt cattagatcc taaggtacct aattgcctag aaaacatgag gatcacccat
     4801 gtctgcaggt cggactctag aaaacatgag gatcacccat gtctgcagta ttcccgggtt
     4861 cattagatct gccgcgcgat cgatagatcc taatcaacct ctggattaca aaatttgtga
     4921 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4981 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     5041 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     5101 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     5161 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     5221 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     5281 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     5341 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     5401 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     5461 cctttgggcc gcctccccgc ctgagatcct ttaagaccaa tgacttacaa ggcagctgta
     5521 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     5581 agacaagatc tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg
     5641 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     5701 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     5761 ttttagtcag tgtggaaaat ctctagcagt agtagttcat gtcatcttat tattcagtat
     5821 ttataacttg caaagaaatg aatatcagag agtgagaggc ccgggttaat taaggaaagg
     5881 gctagatcat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt
     5941 catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac
     6001 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     6061 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     6121 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     6181 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     6241 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     6301 cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca
     6361 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     6421 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     6481 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     6541 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     6601 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     6661 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     6721 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     6781 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     6841 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     6901 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     6961 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     7021 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     7081 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     7141 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     7201 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     7261 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     7321 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     7381 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     7441 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     7501 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     7561 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     7621 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     7681 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     7741 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     7801 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
     7861 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
     7921 tctccccgcg cgttggccga ttcattaatg cagcaagctc atggctgact aatttttttt
     7981 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     8041 ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg cacgacaggt ttcccgactg
     8101 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8161 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8221 tcacacagga aacagctatg acatgattac gaatttcaca aataaagcat ttttttcact
     8281 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     8341 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     8401 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     8461 aagaaattgt atttgttaaa tatgtactac aaacttagta gt
//
