LOCUS       62056_12465        4981 bp DNA     circular SYN 01-JAN-1980
DEFINITION  synthetic circular DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   .
  TITLE     Direct Submission
FEATURES             Location/Qualifiers
     source          1..4981
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        61..364
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        365..568
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early
                     promoter"
     misc_feature    600..665
                     /label=MCS
                     /note="multiple cloning site"
     CDS             711..1643
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    1769..1890
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      complement(1897..2352)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        2379..2483
                     /label=AmpR promoter
     promoter        2485..2842
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     CDS             2877..3668
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     polyA_signal    3903..3950
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      4279..4867
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
       61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
      121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
      601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
      661 gatccggcgg cggagggagt ggaggagggg gaagcggagg cggagggagt atggcttcca
      721 aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg tgggctcgct
      781 gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag aagcacgccg
      841 agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg aggcacgtcg
      901 tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga atgggtaagt
      961 ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac ctcaccgctt
     1021 ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac tggggggctt
     1081 gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc gtccatgctg
     1141 agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag gaggatatcg
     1201 ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc ttcgtcgaga
     1261 ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct gcctacctgg
     1321 agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct cgcgagatcc
     1381 ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac aacgcctacc
     1441 ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg ttcttttcca
     1501 acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag gtgaagggcc
     1561 tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag agcttcgtgg
     1621 agcgcgtgct gaagaacgag cagtaaagcg gccgcgactc tagatcataa tcagccatac
     1681 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     1741 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     1801 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     1861 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
     1921 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
     1981 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
     2041 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
     2101 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
     2161 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
     2221 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
     2281 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
     2341 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     2401 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     2461 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
     2521 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     2581 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
     2641 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
     2701 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     2761 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     2821 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
     2881 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     2941 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3001 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
     3061 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     3121 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     3181 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     3241 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     3301 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     3361 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
     3421 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     3481 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     3541 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     3601 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     3661 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     3721 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     3781 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     3841 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
     3901 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
     3961 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
     4021 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
     4081 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
     4141 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4201 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4261 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     4321 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     4381 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc
     4441 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     4501 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     4561 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     4621 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     4681 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     4741 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     4801 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     4861 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     4921 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
     4981 t
//
