LOCUS       40924_24972        3320 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector phRL-null, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3320)
  AUTHORS   Zhuang Y, Kopps P, Chauvin F, Kenefick KB.
  TITLE     phRL-null Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3320)
  AUTHORS   Zhuang Y, Kopps P, Chauvin F, Kenefick KB.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 3320)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 3320)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods 
            Hollow Rd., Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..3320
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..78
                     /label=multiple cloning region
                     /note="multiple cloning region"
     intron          104..236
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     promoter        281..299
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             309..1241
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(1275..1396)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        1529..1633
                     /label=AmpR promoter
     CDS             1634..2491
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      2665..3253
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 agatctcgag ctctaagctt cacatatgca tgcactagtg gcgcctgtcg acgcgtagaa
       61 ttcacccggg tactgcagaa gttggtcgtg aggcactggg caggtaagta tcaaggttac
      121 aagacaggtt taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg
      181 tttctgatag gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt
      241 ccactcccag ttcaattaca gctcttaagg ctagagtact taatacgact cactataggc
      301 tagccaccat ggcttccaag gtgtacgacc ccgagcaacg caaacgcatg atcactgggc
      361 ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga ctccttcatc aactactatg
      421 attccgagaa gcacgccgag aacgccgtga tttttctgca tggtaacgct gcctccagct
      481 acctgtggag gcacgtcgtg cctcacatcg agcccgtggc tagatgcatc atccctgatc
      541 tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc atatcgcctc ctggatcact
      601 acaagtacct caccgcttgg ttcgagctgc tgaaccttcc aaagaaaatc atctttgtgg
      661 gccacgactg gggggcttgt ctggcctttc actactccta cgagcaccaa gacaagatca
      721 aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga gtcctgggac gagtggcctg
      781 acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg cgagaaaatg gtgcttgaga
      841 ataacttctt cgtcgagacc atgctcccaa gcaagatcat gcggaaactg gagcctgagg
      901 agttcgctgc ctacctggag ccattcaagg agaagggcga ggttagacgg cctaccctct
      961 cctggcctcg cgagatccct ctcgttaagg gaggcaagcc cgacgtcgtc cagattgtcc
     1021 gcaactacaa cgcctacctt cgggccagcg acgatctgcc taagatgttc atcgagtccg
     1081 accctgggtt cttttccaac gctattgtcg agggagctaa gaagttccct aacaccgagt
     1141 tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc tccagatgaa atgggtaagt
     1201 acatcaagag cttcgtggag cgcgtgctga agaacgagca gtaattctag agcggccgct
     1261 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
     1321 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
     1381 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
     1441 ggtgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
     1501 tccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     1561 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     1621 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     1681 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     1741 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     1801 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     1861 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     1921 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     1981 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     2041 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     2101 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     2161 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     2221 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     2281 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     2341 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     2401 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     2461 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     2521 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     2581 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     2641 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     2701 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     2761 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     2821 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     2881 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     2941 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     3001 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     3061 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     3121 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     3181 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     3241 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     3301 ttttgctcac atggctcgac
//
