LOCUS       40924_24957        4843 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Co-reporter vector phRG-TK, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Zhuang Y, Kopps P, Kenefick KB.
  TITLE     phRG-TK Vector
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4843)
  AUTHORS   Zhuang Y, Kopps P, Kenefick KB.
  TITLE     Direct Submission
  JOURNAL   Submitted (20-MAR-2001) Technical Writing, Promega Corporation, 2800
            Woods Hollow Rd., Madison, WI 53711-5399, USA
REFERENCE   3  (bases 1 to 4843)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4843)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (20-MAR-2001) Technical Writing, Promega Corporation, 2800 Woods 
            Hollow Rd., Madison, WI 53711-5399, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        43..794
                     /label=HSV TK promoter
                     /note="herpes simplex virus thymidine kinase promoter"
     CDS             830..1762
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     polyA_signal    complement(1806..1927)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2086..2105)
                     /label=RV primer 4
                     /note="RV primer 4"
     misc_feature    2343
                     /label=ColE1-derived plasmid replication origin
                     /note="ColE1-derived plasmid replication origin"
     rep_origin      complement(2346..2934)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(3108..3965)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3966..4070)
                     /label=AmpR promoter
     rep_origin      4097..4552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4683..4731
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4745..4836
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc taaatgagtc ttcggacctc
       61 gcgggggccg cttaagcggt ggttagggtt tgtctgacgc ggggggaggg ggaaggaacg
      121 aaacactctc attcggaggc ggctcggggt ttggtcttgg tggccacggg cacgcagaag
      181 agcgccgcga tcctcttaag cacccccccg ccctccgtgg aggcgggggt ttggtcggcg
      241 ggtggtaact ggcgggccgc tgactcgggc gggtcgcgcg ccccagagtg tgaccttttc
      301 ggtctgctcg cagacccccg ggcggcgccg ccgcggcggc gacgggctcg ctgggtccta
      361 ggctccatgg ggaccgtata cgtggacagg ctctggagca tccgcacgac tgcggtgata
      421 ttaccggaga ccttctgcgg gacgagccgg gtcacgcggc tgacgcggag cgtccgttgg
      481 gcgacaaaca ccaggacggg gcacaggtac actatcttgt cacccggagg cgcgagggac
      541 tgcaggagct tcagggagtg gcgcagctgc ttcatccccg tggcccgttg ctcgcgtttg
      601 ctggcggtgt ccccggaaga aatatatttg catgtcttta gttctatgat gacacaaacc
      661 ccgcccagcg tcttgtcatt ggcgaattcg aacacgcaga tgcagtcggg gcggcgcggt
      721 cccaggtcca cttcgcatat taaggtgacg cgtgtggcct cgaacaccga gcgaccctgc
      781 agcgacccgc ttaaaagctt ggcattccgg tactgttggt aaagccacca tggcttccaa
      841 ggtgtacgac cccgagcaac gcaaacgcat gatcactggg cctcagtggt gggctcgctg
      901 caagcaaatg aacgtgctgg actccttcat caactactat gattccgaga agcacgccga
      961 gaacgccgtg atttttctgc atggtaacgc tgcctccagc tacctgtgga ggcacgtcgt
     1021 gcctcacatc gagcccgtgg ctagatgcat catccctgat ctgatcggaa tgggtaagtc
     1081 cggcaagagc gggaatggct catatcgcct cctggatcac tacaagtacc tcaccgcttg
     1141 gttcgagctg ctgaaccttc caaagaaaat catctttgtg ggccacgact ggggggcttg
     1201 tctggccttt cactactcct acgagcacca agacaagatc aaggccatcg tccatgctga
     1261 gagtgtcgtg gacgtgatcg agtcctggga cgagtggcct gacatcgagg aggatatcgc
     1321 cctgatcaag agcgaagagg gcgagaaaat ggtgcttgag aataacttct tcgtcgagac
     1381 catgctccca agcaagatca tgcggaaact ggagcctgag gagttcgctg cctacctgga
     1441 gccattcaag gagaagggcg aggttagacg gcctaccctc tcctggcctc gcgagatccc
     1501 tctcgttaag ggaggcaagc ccgacgtcgt ccagattgtc cgcaactaca acgcctacct
     1561 tcgggccagc gacgatctgc ctaagatgtt catcgagtcc gaccctgggt tcttttccaa
     1621 cgctattgtc gagggagcta agaagttccc taacaccgag ttcgtgaagg tgaagggcct
     1681 ccacttcagc caggaggacg ctccagatga aatgggtaag tacatcaaga gcttcgtgga
     1741 gcgcgtgctg aagaacgagc agtaattcta gagtcggggc ggccggccgc ttcgagcaga
     1801 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
     1861 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
     1921 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
     1981 ggttttttaa agcaagtaaa acctctacaa atgtggtaaa atcgataagg atccgtcgac
     2041 cgatgccctt gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta
     2101 tcgtcgccgc acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag
     2161 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     2221 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     2281 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     2341 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     2401 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     2461 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     2521 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     2581 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     2641 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     2701 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     2761 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     2821 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     2881 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     2941 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     3001 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     3061 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     3121 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     3181 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     3241 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     3301 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     3361 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     3421 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     3481 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     3541 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     3601 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     3661 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     3721 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     3781 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     3841 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     3901 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     3961 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     4021 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     4081 cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     4141 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     4201 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     4261 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     4321 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     4381 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     4441 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     4501 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttgccattcg
     4561 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     4621 cagcccaagc taccatgata agtaagtaat attaaggtac gggaggtact tggagcggcc
     4681 gcaataaaat atctttattt tcattacatc tgtgtgttgg ttttttgtgt gaatcgatag
     4741 tactaacata cgctctccat caaaacaaaa cgaaacaaaa caaactagca aaataggctg
     4801 tccccagtgc aagtgcaggt gccagaacat ttctctatcg ata
//
