LOCUS       40924_24942        4232 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Reporter vector phRG(R2.1), complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4232)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Rapid Response (TM) Reporter Vectors Technical Manual, TM242
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4232)
  AUTHORS   Almond BD, Chauvin F, Kenefick KB.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-NOV-2003) Scientific Communications, Promega 
            Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
REFERENCE   3  (bases 1 to 4232)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4232)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (26-NOV-2003) Scientific Communications, Promega Corporation, 2800 
            Woods Hollow Rd., Madison, WI 53711, USA"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4232
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..58
                     /label=multiple cloning region
                     /note="multiple cloning region"
     CDS             88..1020
                     /codon_start=1
                     /label=hRluc
                     /note="Renilla luciferase"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             1024..1143
                     /codon_start=1
                     /label=hPEST
                     /note="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    complement(1195..1316)
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     1475..1494
                     /label=reporter vector primer 4 (RVPrimer4)
                     /note="reporter vector primer 4 (RVPrimer4)"
     rep_origin      1732
                     /label=ColE1-derived
                     /note="ColE1-derived"
     rep_origin      complement(1735..2323)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2497..3354)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3355..3459)
                     /label=AmpR promoter
     rep_origin      3486..3941
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     polyA_signal    4072..4120
                     /label=poly(A) signal
                     /note="synthetic polyadenylation signal"
     misc_feature    4134..4225
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from
                     the human alpha-2 globin gene"
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg gctcgagatc tgcgatctaa gtaagcttgg
       61 cattccggta ctgttggtaa agccaccatg gcttccaagg tgtacgaccc cgagcaacgc
      121 aaacgcatga tcactgggcc tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac
      181 tccttcatca actactatga ttccgagaag cacgccgaga acgccgtgat ttttctgcat
      241 ggtaacgctg cctccagcta cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct
      301 agatgcatca tccctgatct gatcggaatg ggtaagtccg gcaagagcgg gaatggctca
      361 tatcgcctcc tggatcacta caagtacctc accgcttggt tcgagctgct gaaccttcca
      421 aagaaaatca tctttgtggg ccacgactgg ggggcttgtc tggcctttca ctactcctac
      481 gagcaccaag acaagatcaa ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag
      541 tcctgggacg agtggcctga catcgaggag gatatcgccc tgatcaagag cgaagagggc
      601 gagaaaatgg tgcttgagaa taacttcttc gtcgagacca tgctcccaag caagatcatg
      661 cggaaactgg agcctgagga gttcgctgcc tacctggagc cattcaagga gaagggcgag
      721 gttagacggc ctaccctctc ctggcctcgc gagatccctc tcgttaaggg aggcaagccc
      781 gacgtcgtcc agattgtccg caactacaac gcctaccttc gggccagcga cgatctgcct
      841 aagatgttca tcgagtccga ccctgggttc ttttccaacg ctattgtcga gggagctaag
      901 aagttcccta acaccgagtt cgtgaaggtg aagggcctcc acttcagcca ggaggacgct
      961 ccagatgaaa tgggtaagta catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag
     1021 aattctcacg gcttccctcc cgaggtggag gagcaggccg ccggcaccct gcccatgagc
     1081 tgcgcccagg agagcggcat ggatagacac cctgctgctt gcgccagcgc caggatcaac
     1141 gtctaaggcc gcgactctag agtcggggcg gccggccgct tcgagcagac atgataagat
     1201 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     1261 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     1321 acaacaattg cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaaa
     1381 gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga tccgtcgacc gatgcccttg
     1441 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     1501 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     1561 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     1621 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     1681 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     1741 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     1801 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     1861 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     1921 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     1981 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2041 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2101 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2161 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2221 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2281 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     2341 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     2401 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     2461 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     2521 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     2581 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     2641 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     2701 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     2761 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     2821 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     2881 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     2941 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3001 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3061 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3121 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3181 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3241 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3301 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     3361 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     3421 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     3481 acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     3541 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     3601 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     3661 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     3721 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     3781 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     3841 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     3901 atttaacgcg aattttaaca aaatattaac gcttacaatt tgccattcgc cattcaggct
     3961 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct
     4021 accatgataa gtaagtaata ttaaggtacg ggaggtactt ggagcggccg caataaaata
     4081 tctttatttt cattacatct gtgtgttggt tttttgtgtg aatcgatagt actaacatac
     4141 gctctccatc aaaacaaaac gaaacaaaac aaactagcaa aataggctgt ccccagtgca
     4201 agtgcaggtg ccagaacatt tctctatcga ta
//
