LOCUS       40924_24433        9464 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector phEF1a-rtTA-2A-Hyg-FF5, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9464)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9464)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 9464)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9464)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9464
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    169..224
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(585..601)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    609..625
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(633..663)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    678..699
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    872..1708
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(1738..1756)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1777..1793)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1801..1817)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(1825..1855)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(1870..1891)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2179..2767)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(2941..3798)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(3799..3903)
                     /label=AmpR promoter
     rep_origin      complement(3929..4384)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     4526..4542
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        4552..4570
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    4585..5388
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    5467..5492
                     /label=SA
                     /note="splice acceptor site"
     protein_bind    6164..6184
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        6209..7381
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     protein_bind    7403..7427
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             7441..8184
                     /codon_start=1
                     /label=rtTA-Advanced
                     /note="improved tetracycline-controlled transactivator"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             8194..8250
                     /codon_start=1
                     /label=P2A
                     /note="2A peptide from porcine teschovirus-1 polyprotein"
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8263..9285
                     /codon_start=1
                     /label=HygR
                     /note="aminoglycoside phosphotransferase from E. coli"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     misc_feature    9300..9321
                     /label=FF5
                     /note="FF5"
     misc_feature    9322..9343
                     /label=FF5
                     /note="FF5"
     misc_feature    9344..9365
                     /label=FF5
                     /note="FF5"
     misc_feature    9366..9387
                     /label=FF5
                     /note="FF5"
ORIGIN
        1 ttgtacaaag tggtacgcgt gaattcactc ctcaggtgca ggctgcctat cagaaggtgg
       61 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
      121 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
      181 ttattttcat tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg
      241 ggagggcaaa tcatttaaaa catcagaatg agtatttggt ttagagtttg gcaacatatg
      301 cccatatgct ggctgccatg aacaaaggtt ggctataaag aggtcatcag tatatgaaac
      361 agccccctgc tgtccattcc ttattccata gaaaagcctt gacttgaggt tagatttttt
      421 ttatattttg ttttgtgtta tttttttctt taacatccct aaaattttcc ttacatgttt
      481 tactagccag atttttcctc ctctcctgac tactcccagt catagctgtc cctcttctct
      541 tatggagatc cctcgacctg cagcccaagc ttggcgtaat catggtcata gctgtttcct
      601 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
      661 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
      721 gctttccagt cgggaaacct gtcgtgccag cggatcgaca gtactaagct tggtgcgttt
      781 ttatgcttgt agtattgtat aatgttttta agatccttaa ttaataggga taacagggta
      841 atctcgaggt ttaaacgtcg acgcggccgc tactagggac aggattggtg acagaaaagc
      901 cccatcctta ggcctcctcc ttcctagtct cctgatattg ggtctaaccc ccacctcctg
      961 ttaggcagat tccttatctg gtgacacacc cccatttcct ggagccatct ctctccttgc
     1021 cagaacctct aaggtttgct tacgatggag ccagagagga tcctgggagg gagagcttgg
     1081 cagggggtgg gagggaaggg ggggatgcgt gacctgcccg gttctcagtg gccaccctgc
     1141 gctaccctct cccagaacct gagctgctct gacgcggctg tctggtgcgt ttcactgatc
     1201 ctggtgctgc agcttcctta cacttcccaa gaggagaagc agtttggaaa aacaaaatca
     1261 gaataagttg gtcctgagtt ctaactttgg ctcttcacct ttctagtccc caatttatat
     1321 tgttcctccg tgcgtcagtt ttacctgtga gataaggcca gtagccagcc ccgtcctggc
     1381 agggctgtgg tgaggagggg ggtgtccgtg tggaaaactc cctttgtgag aatggtgcgt
     1441 cctaggtgtt caccaggtcg tggccgcctc tactcccttt ctctttctcc atccttcttt
     1501 ccttaaagag tccccagtgc tatctgggac atattcctcc gcccagagca gggtcccgct
     1561 tccctaaggc cctgctctgg gcttctgggt ttgagtcctt ggcaagccca ggagaggcgc
     1621 tcaggcttcc ctgtccccct tcctcgtcca ccatctcatg cccctggctc tcctgcccct
     1681 tccctacagg ggttcctggc tctgctctcc accgcggtgg agctccagct tttgttccct
     1741 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     1801 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     1861 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     1921 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     1981 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     2041 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     2101 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     2161 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     2221 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     2281 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     2341 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     2401 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     2461 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     2521 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     2581 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     2641 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2701 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2761 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2821 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     2881 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     2941 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     3001 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     3061 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     3121 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     3181 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     3241 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     3301 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     3361 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     3421 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     3481 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     3541 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     3601 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     3661 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     3721 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     3781 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     3841 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     3901 gcgcacattt ccccgaaaag tgccacctaa attgtaagcg ttaatatttt gttaaaattc
     3961 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc
     4021 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag
     4081 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc
     4141 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa
     4201 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg
     4261 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt
     4321 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc
     4381 gcgtcccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     4441 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     4501 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgagcgcgc gtaatacgac
     4561 tcactatagg gcgaattggg tacctgcttt ctctgaccag cattctctcc cctgggcctg
     4621 tgccgctttc tgtctgcagc ttgtggcctg ggtcacctct acggctggcc cagatccttc
     4681 cctgccgcct ccttcaggtt ccgtcttcct ccactccctc ttccccttgc tctctgctgt
     4741 gttgctgccc aaggatgctc tttccggagc acttccttct cggcgctgca ccacgtgatg
     4801 tcctctgagc ggatcctccc cgtgtctggg tcctctccgg gcatctctcc tccctcaccc
     4861 aaccccatgc cgtcttcact cgctgggttc ccttttcctt ctccttctgg ggcctgtgcc
     4921 atctctcgtt tcttaggatg gccttctccg acggatgtct cccttgcgtc ccgcctcccc
     4981 ttcttgtagg cctgcatcat caccgttttt ctggacaacc ccaaagtacc ccgtctccct
     5041 ggctttagcc acctctccat cctcttgctt tctttgcctg gacaccccgt tctcctgtgg
     5101 attcgggtca cctctcactc ctttcatttg ggcagctccc ctacccccct tacctctcta
     5161 gtctgtgcta gctcttccag ccccctgtca tggcatcttc caggggtccg agagctcagc
     5221 tagtcttctt cctccaaccc gggcccctat gtccacttca ggacagcatg tttgctgcct
     5281 ccagggatcc tgtgtccccg agctgggacc accttatatt cccagggccg gttaatgtgg
     5341 ctctggttct gggtactttt atctgtcccc tccaccccac agtggggcaa gcttattacc
     5401 ctgttatccc taaccggttt accgagctct tattggtttt caaacttcat tgactgtgcc
     5461 aagcttctga cctcttctct tcctcccaca gggcctcgag acaattgcat cgatggtacc
     5521 agtgaaagtc tgcaatgaat tcgagttggc gcgcctgtca ttctaaatct ctctttcagc
     5581 ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
     5641 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
     5701 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
     5761 cccctagcgg gggagggacg taattacatc cctgggggct ttgggagggg gctgtccccg
     5821 tgagctccca ggcgcgcctg tcattctaaa tctctctttc agcctaaagc tttttccccg
     5881 tatcccccca ggtgtctgca ggctcaaaga gcagcgagaa gcgttcagag gaaagcgatc
     5941 ccgtgccacc ttccccgtgc ccgggctgtc cccgcacgct gccggctcgg ggatgcgggg
     6001 ggagcgccgg accggagcgg agccccgggc ggctcgctgc tgccccctag cgggggaggg
     6061 acgtaattac atccctgggg gctttggggg ggggctgtcc ccgtgagctc ccaggcgcgc
     6121 caactcggta ccgtatcgat gtcgacgtta acgctagtga tatcaacttt gtatagaaaa
     6181 gttggctccg aattcgccct tattaattgg ctccggtgcc cgtcagtggg cagagcgcac
     6241 atcgcccaca gtccccgaga agttgtgggg aggggtcggc aattgaaccg gtgcctagag
     6301 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     6361 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     6421 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     6481 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     6541 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     6601 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     6661 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     6721 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     6781 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     6841 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     6901 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     6961 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     7021 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     7081 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     7141 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     7201 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     7261 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     7321 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     7381 ggatcccaag ggcgaattcg acccaagttt gtacaaaaaa gcaggctgat taccggtacc
     7441 atgtctagac tggacaagag caaagtcata aacggcgctc tggaattact caatggagtc
     7501 ggtatcgaag gcctgacgac aaggaaactc gctcaaaagc tgggagttga gcagcctacc
     7561 ctgtactggc acgtgaagaa caagcgggcc ctgctcgatg ccctgccaat cgagatgctg
     7621 gacaggcatc atacccactt ctgccccctg gaaggcgagt catggcaaga ctttctgcgg
     7681 aacaacgcca agtcattccg ctgtgctctc ctctcacatc gcgacggggc taaagtgcat
     7741 ctcggcaccc gcccaacaga gaaacagtac gaaaccctgg aaaatcagct cgcgttcctg
     7801 tgtcagcaag gcttctccct ggagaacgca ctgtacgctc tgtccgccgt gggccacttt
     7861 acactgggct gcgtattgga ggaacaggag catcaagtag caaaagagga aagagagaca
     7921 cctaccaccg attctatgcc cccacttctg agacaagcaa ttgagctgtt cgaccggcag
     7981 ggagccgaac ctgccttcct tttcggcctg gaactaatca tatgtggcct ggagaaacag
     8041 ctaaagtgcg aaagcggcgg gccggccgac gcccttgacg attttgactt agacatgctc
     8101 ccagccgatg cccttgacga ctttgacctt gatatgctgc ctgctgacgc tcttgacgat
     8161 tttgaccttg acatgctccc cgggggatct ggcgccacca acttctctct gctgaagcag
     8221 gccggcgacg tggaggagaa cccaggccca actagtgcta gaatgaaaaa gcctgaactc
     8281 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     8341 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     8401 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     8461 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt cagcgagagc
     8521 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     8581 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     8641 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     8701 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     8761 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     8821 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     8881 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     8941 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     9001 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     9061 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     9121 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     9181 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     9241 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaataggc ggccgcaaaa
     9301 agcactctga tttgacaatt aaagcactct gatttgacaa ttaaagcact ctgatttgac
     9361 aattaaagca ctctgatttg acaattaaag cttcgaattc tgcagtcgac ggtaccgcgg
     9421 gcccgggatc caccggatct agataactga tctacccagc tttc
//
