LOCUS       40924_24428        9487 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector phEF1a-EYFP-2A-Hyg, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9487)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W, Li Z, Weiss R, Xie Z.
  TITLE     Modular construction of mammalian gene circuits using TALE 
            transcriptional repressors
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9487)
  AUTHORS   Li Y, Jiang Y, Chen H, Liao W.
  TITLE     Direct Submission
  JOURNAL   Submitted (03-SEP-2014) Bioinformatics Division/Center for Synthetic
REFERENCE   3  (bases 1 to 9487)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 9487)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (03-SEP-2014) Bioinformatics Division/Center for Synthetic "
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     ##Assembly-Data-START##
            Sequencing Technology :: Sanger dideoxy sequencing 
            ##Assembly-Data-END##
FEATURES             Location/Qualifiers
     source          1..9487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..21
                     /label=attB4
                     /note="core recombination site for the Gateway(R) BP
                     reaction"
     promoter        46..1218
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation
                     factor EF-1-alpha"
     protein_bind    1241..1265
                     /label=attB1
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1286..2002
                     /codon_start=1
                     /label=mEYFP
                     /note="enhanced YFP with monomerizing A206K mutation
                     (Zacharias et al., 2002)"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     misc_feature    2009..2062
                     /label=2A
                     /note="2A"
     CDS             2009..2062
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             2069..3103
                     /codon_start=1
                     /label=HygR
                     /note="hygromycin B phosphotransferase"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPDRE
                     MGEAN"
     protein_bind    complement(3189..3213)
                     /label=attB2
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3214..3338)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     polyA_signal    3493..3548
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3909..3925)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3933..3949
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3957..3987)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4002..4023
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     misc_feature    4196..5032
                     /label=HA-R
                     /note="right homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     promoter        complement(5062..5080)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5101..5117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(5125..5141)
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5149..5179)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(5194..5215)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(5503..6091)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6265..7122)
                     /codon_start=1
                     /label=AmpR
                     /note="beta-lactamase"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7123..7227)
                     /label=AmpR promoter
     rep_origin      complement(7253..7708)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     primer_bind     7850..7866
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        7876..7894
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    7909..8712
                     /label=HA-L
                     /note="left homology arm from the adeno-associated virus 
                     integration site (AAVS1) within intron 1 of the human 
                     PPP1R12C gene"
     misc_feature    8791..8816
                     /label=SA
                     /note="splice acceptor site"
ORIGIN
        1 caactttgta tagaaaagtt ggctccgaat tcgcccttat taattggctc cggtgcccgt
       61 cagtgggcag agcgcacatc gcccacagtc cccgagaagt tgtggggagg ggtcggcaat
      121 tgaaccggtg cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg
      181 ctccgccttt ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac
      241 gttctttttc gcaacgggtt tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc
      301 gggcctggcc tctttacggg ttatggccct tgcgtgcctt gaattacttc cacctggctg
      361 cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct
      421 tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcctgg gcgctggggc
      481 cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga taagtctcta
      541 gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga tagtcttgta
      601 aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg cggcgacggg
      661 gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga
      721 atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct cgcgccgccg
      781 tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa
      841 agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga
      901 gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc agccgtcgct
      961 tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt
     1021 tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt tccccacact
     1081 gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc cttggaattt
     1141 gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt tcaaagtttt
     1201 tttcttccat ttcaggtgga tcccaagggc gaattcgacc ccaagtttgt acaaaaaagc
     1261 aggctgagcc accatgtcgg aattcatggt gagcaagggc gaggagctgt tcaccggggt
     1321 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     1381 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     1441 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt
     1501 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1561 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1621 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1681 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1741 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat
     1801 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1861 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     1921 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1981 cggcatggac gagctgtaca agcaattgga gggccgcggc agcctgctga cctgcggcga
     2041 cgtggaggaa aaccccggcc ccaccggtat gaaaaagcct gaactcaccg cgacgtctgt
     2101 cgagaagttt ctgatcgaaa agttcgacag cgtctccgac ctgatgcagc tctcggaggg
     2161 cgaagaatct cgtgctttca gcttcgatgt aggagggcgt ggatatgtcc tgcgggtaaa
     2221 tagctgcgcc gatggtttct acaaagatcg ttatgtttat cggcactttg catcggccgc
     2281 gctcccgatt ccggaagtgc ttgacattgg ggaattcagc gagagcctga cctattgcat
     2341 ctcccgccgt gcacagggtg tcacgttgca agacctgcct gaaaccgaac tgcccgctgt
     2401 tctgcagccg gtcgcggagg ccatggatgc gatcgctgcg gccgatctta gccagacgag
     2461 cgggttcggc ccattcggac cgcaaggaat cggtcaatac actacatggc gtgatttcat
     2521 atgcgcgatt gctgatcccc atgtgtatca ctggcaaact gtgatggacg acaccgtcag
     2581 tgcgtccgtc gcgcaggctc tcgatgagct gatgctttgg gccgaggact gccccgaagt
     2641 ccggcacctc gtgcacgcgg atttcggctc caacaatgtc ctgacggaca atggccgcat
     2701 aacagcggtc attgactgga gcgaggcgat gttcggggat tcccaatacg aggtcgccaa
     2761 catcttcttc tggaggccgt ggttggcttg tatggagcag cagacgcgct acttcgagcg
     2821 gaggcatccg gagcttgcag gatcgccgcg gctccgggcg tatatgctcc gcattggtct
     2881 tgaccaactc tatcagagct tggttgacgg caatttcgat gatgcagctt gggcgcaggg
     2941 tcgatgcgac gcaatcgtcc gatccggagc cgggactgtc gggcgtacac aaatcgcccg
     3001 cagaagcgcg gccgtctgga ccgatggctg tgtagaagta ctcgccgata gtggaaaccg
     3061 acgccccagc actcgtccgg atcgggagat gggggaggct aacccatggt ctggtggcgg
     3121 tggctcgggc ggaggtgggt cgggtggcgg cggatcaaag cttggtggcg gctcaactag
     3181 ttaataccac ccagctttct tgtacaaagt ggtcatagtg actggatatg ttgtgtttta
     3241 cagtattatg tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca
     3301 ttttacgttt ctcgttcagc tttcttgtac aaagtggtac gcgtgaattc actcctcagg
     3361 tgcaggctgc ctatcagaag gtggtggctg gtgtggccaa tgccctggct cacaaatacc
     3421 actgagatct ttttccctct gccaaaaatt atggggacat catgaagccc cttgagcatc
     3481 tgacttctgg ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt
     3541 gtctctcact cggaaggaca tatgggaggg caaatcattt aaaacatcag aatgagtatt
     3601 tggtttagag tttggcaaca tatgcccata tgctggctgc catgaacaaa ggttggctat
     3661 aaagaggtca tcagtatatg aaacagcccc ctgctgtcca ttccttattc catagaaaag
     3721 ccttgacttg aggttagatt ttttttatat tttgttttgt gttatttttt tctttaacat
     3781 ccctaaaatt ttccttacat gttttactag ccagattttt cctcctctcc tgactactcc
     3841 cagtcatagc tgtccctctt ctcttatgga gatccctcga cctgcagccc aagcttggcg
     3901 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     3961 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     4021 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc
     4081 gacagtacta agcttggtgc gtttttatgc ttgtagtatt gtataatgtt tttaagatcc
     4141 ttaattaata gggataacag ggtaatctcg aggtttaaac gtcgacgcgg ccgctactag
     4201 ggacaggatt ggtgacagaa aagccccatc cttaggcctc ctccttccta gtctcctgat
     4261 attgggtcta acccccacct cctgttaggc agattcctta tctggtgaca cacccccatt
     4321 tcctggagcc atctctctcc ttgccagaac ctctaaggtt tgcttacgat ggagccagag
     4381 aggatcctgg gagggagagc ttggcagggg gtgggaggga agggggggat gcgtgacctg
     4441 cccggttctc agtggccacc ctgcgctacc ctctcccaga acctgagctg ctctgacgcg
     4501 gctgtctggt gcgtttcact gatcctggtg ctgcagcttc cttacacttc ccaagaggag
     4561 aagcagtttg gaaaaacaaa atcagaataa gttggtcctg agttctaact ttggctcttc
     4621 acctttctag tccccaattt atattgttcc tccgtgcgtc agttttacct gtgagataag
     4681 gccagtagcc agccccgtcc tggcagggct gtggtgagga ggggggtgtc cgtgtggaaa
     4741 actccctttg tgagaatggt gcgtcctagg tgttcaccag gtcgtggccg cctctactcc
     4801 ctttctcttt ctccatcctt ctttccttaa agagtcccca gtgctatctg ggacatattc
     4861 ctccgcccag agcagggtcc cgcttcccta aggccctgct ctgggcttct gggtttgagt
     4921 ccttggcaag cccaggagag gcgctcaggc ttccctgtcc cccttcctcg tccaccatct
     4981 catgcccctg gctctcctgc cccttcccta caggggttcc tggctctgct ctccaccgcg
     5041 gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg
     5101 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
     5161 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
     5221 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
     5281 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
     5341 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     5401 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     5461 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     5521 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     5581 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     5641 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     5701 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     5761 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     5821 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     5881 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     5941 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     6001 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     6061 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     6121 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     6181 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     6241 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     6301 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     6361 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     6421 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     6481 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     6541 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     6601 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     6661 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     6721 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     6781 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     6841 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
     6901 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     6961 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     7021 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     7081 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     7141 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     7201 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta
     7261 agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     7321 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     7381 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     7441 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     7501 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     7561 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     7621 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     7681 gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg
     7741 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
     7801 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
     7861 gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacctg ctttctctga
     7921 ccagcattct ctcccctggg cctgtgccgc tttctgtctg cagcttgtgg cctgggtcac
     7981 ctctacggct ggcccagatc cttccctgcc gcctccttca ggttccgtct tcctccactc
     8041 cctcttcccc ttgctctctg ctgtgttgct gcccaaggat gctctttccg gagcacttcc
     8101 ttctcggcgc tgcaccacgt gatgtcctct gagcggatcc tccccgtgtc tgggtcctct
     8161 ccgggcatct ctcctccctc acccaacccc atgccgtctt cactcgctgg gttccctttt
     8221 ccttctcctt ctggggcctg tgccatctct cgtttcttag gatggccttc tccgacggat
     8281 gtctcccttg cgtcccgcct ccccttcttg taggcctgca tcatcaccgt ttttctggac
     8341 aaccccaaag taccccgtct ccctggcttt agccacctct ccatcctctt gctttctttg
     8401 cctggacacc ccgttctcct gtggattcgg gtcacctctc actcctttca tttgggcagc
     8461 tcccctaccc cccttacctc tctagtctgt gctagctctt ccagccccct gtcatggcat
     8521 cttccagggg tccgagagct cagctagtct tcttcctcca acccgggccc ctatgtccac
     8581 ttcaggacag catgtttgct gcctccaggg atcctgtgtc cccgagctgg gaccacctta
     8641 tattcccagg gccggttaat gtggctctgg ttctgggtac ttttatctgt cccctccacc
     8701 ccacagtggg gcaagcttat taccctgtta tccctaaccg gtttaccgag ctcttattgg
     8761 ttttcaaact tcattgactg tgccaagctt ctgacctctt ctcttcctcc cacagggcct
     8821 cgagacaatt gcatcgatgg taccagtgaa agtctgcaat gaattcgagt tggcgcgcct
     8881 gtcattctaa atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc
     8941 aggctcaaag agcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg
     9001 cccgggctgt ccccgcacgc tgccggctcg gggatgcggg gggagcgccg gaccggagcg
     9061 gagccccggg cggctcgctg ctgcccccta gcgggggagg gacgtaatta catccctggg
     9121 ggctttggga gggggctgtc cccgtgagct cccaggcgcg cctgtcattc taaatctctc
     9181 tttcagccta aagctttttc cccgtatccc cccaggtgtc tgcaggctca aagagcagcg
     9241 agaagcgttc agaggaaagc gatcccgtgc caccttcccc gtgcccgggc tgtccccgca
     9301 cgctgccggc tcggggatgc ggggggagcg ccggaccgga gcggagcccc gggcggctcg
     9361 ctgctgcccc ctagcggggg agggacgtaa ttacatccct gggggctttg ggggggggct
     9421 gtccccgtga gctcccaggc gcgccaactc ggtaccgtat cgatgtcgac gttaacgcta
     9481 gtgatat
//
